Construction of Overexpression Vector with <i>TYR7523</i> Gene and Its Effect on Browning in <i>Macrocybe gigantea</i>
<i>Macrocybe gigantea</i> is a rare high-temperature edible fungus known for its resistance to browning. Previous studies suggested that the anti-browning property of the SCAU4 strain might be associated with low expression levels of the <i>TYR7523</i> gene. In this study, an...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-03-01
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| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/11/3/216 |
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| Summary: | <i>Macrocybe gigantea</i> is a rare high-temperature edible fungus known for its resistance to browning. Previous studies suggested that the anti-browning property of the SCAU4 strain might be associated with low expression levels of the <i>TYR7523</i> gene. In this study, an overexpression vector for the <i>TYR7523</i> gene was constructed and introduced into SCAU4 mycelium using an Agrobacterium-mediated transformation method. After three rounds of hygromycin resistance screening, successful transformants were identified through PCR amplification and validated by qRT-PCR analysis, confirming a 3.47-fold upregulation of <i>TYR7523</i> expression. The overexpression strain <i>OE7523</i> was compared with the wild-type SCAU4 strain in terms of growth rate, browning degree, and tyrosinase activity. Although there was no significant difference in growth rate on the mother culture medium, <i>OE7523</i> showed faster growth on the stock culture and mycelium culture medium. In the late storage period, <i>OE7523</i> exhibited a higher browning degree and tyrosinase activity than SCAU4, suggesting a potential role of <i>TYR7523</i> in fruiting body browning. Physiological analyses indicated that low <i>TYR7523</i> expression may contribute to storage tolerance, while high expression influenced postharvest browning and preservation duration. The results provide data support for further study on the function of <i>TYR7523</i> gene of <i>Macrocybe gigantea</i>. |
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| ISSN: | 2309-608X |