PGP Attributes of Non-rhizobial Bacterial Endophytes from Root Nodules of Wild Leguminous Plant Tephrosia Purpurea (L) Pers.

Global agriculture practices rely heavily on the application of fungicides, pesticides and synthetic fertilizers. Several bacterial strains were isolated from root nodules and screened for plant growth-promoting (PGP) activities. The selected isolates were characterized biochemically and identified...

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Main Authors: Harshita Vashistha, Prashant Kumar, Sandeep Kumar
Format: Article
Language:English
Published: Action for Sustainable Efficacious Development and Awareness 2024-09-01
Series:Environment Conservation Journal
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Online Access:https://journal.environcj.in/index.php/ecj/article/view/2759
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author Harshita Vashistha
Prashant Kumar
Sandeep Kumar
author_facet Harshita Vashistha
Prashant Kumar
Sandeep Kumar
author_sort Harshita Vashistha
collection DOAJ
description Global agriculture practices rely heavily on the application of fungicides, pesticides and synthetic fertilizers. Several bacterial strains were isolated from root nodules and screened for plant growth-promoting (PGP) activities. The selected isolates were characterized biochemically and identified by 16S rRNA sequencing. The ability of bacterial isolates to produce siderophores, phosphate solubilization, production of indole acetic acid (IAA) and other characteristics of plant growth promoting (PGP)activities were evaluated from the root nodules of a wild leguminous plant, Tephrosia purpurea (L.) Pers. Based on the results, the isolates were determined as Bacillus altitudinis and Pseudomonas azotoformans. The bacterial consortium consisting of B. altitudinis + P. azotoformans exhibited a substantial enhancement in both root length (35.55%), and shoot length (43.2%) when compared to the control. This study concludes that utilization of the beneficial traits of these non-rhizobial nodule endophytes bacteria improve nodulation biomass accumulation and plant vegetative growth parameters in T. purpurea. The present research has the potential to advance the adoption of eco-friendly agricultural practices and provide an alternative substitute for traditional chemical fertilizers.
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spelling doaj-art-a4ff1fcf1bf841b5bdc64a0c7205b87f2024-12-21T07:38:50ZengAction for Sustainable Efficacious Development and AwarenessEnvironment Conservation Journal0972-30992278-51242024-09-0125410.36953/ECJ.27592833PGP Attributes of Non-rhizobial Bacterial Endophytes from Root Nodules of Wild Leguminous Plant Tephrosia Purpurea (L) Pers.Harshita Vashistha0Prashant Kumar1Sandeep Kumar2Department of Botany and Microbiology, Gurukula Kangri (Deemed to be University), Haridwar, Uttarakhand, IndiaDepartment of Botany and Microbiology, Gurukula Kangri (Deemed to be University), Haridwar, Uttarakhand, IndiaDepartment of Botany and Microbiology Global agriculture practices rely heavily on the application of fungicides, pesticides and synthetic fertilizers. Several bacterial strains were isolated from root nodules and screened for plant growth-promoting (PGP) activities. The selected isolates were characterized biochemically and identified by 16S rRNA sequencing. The ability of bacterial isolates to produce siderophores, phosphate solubilization, production of indole acetic acid (IAA) and other characteristics of plant growth promoting (PGP)activities were evaluated from the root nodules of a wild leguminous plant, Tephrosia purpurea (L.) Pers. Based on the results, the isolates were determined as Bacillus altitudinis and Pseudomonas azotoformans. The bacterial consortium consisting of B. altitudinis + P. azotoformans exhibited a substantial enhancement in both root length (35.55%), and shoot length (43.2%) when compared to the control. This study concludes that utilization of the beneficial traits of these non-rhizobial nodule endophytes bacteria improve nodulation biomass accumulation and plant vegetative growth parameters in T. purpurea. The present research has the potential to advance the adoption of eco-friendly agricultural practices and provide an alternative substitute for traditional chemical fertilizers. https://journal.environcj.in/index.php/ecj/article/view/2759Wild LegumesPGPRBiochemical TestVegetative GrowthPot Trial
spellingShingle Harshita Vashistha
Prashant Kumar
Sandeep Kumar
PGP Attributes of Non-rhizobial Bacterial Endophytes from Root Nodules of Wild Leguminous Plant Tephrosia Purpurea (L) Pers.
Environment Conservation Journal
Wild Legumes
PGPR
Biochemical Test
Vegetative Growth
Pot Trial
title PGP Attributes of Non-rhizobial Bacterial Endophytes from Root Nodules of Wild Leguminous Plant Tephrosia Purpurea (L) Pers.
title_full PGP Attributes of Non-rhizobial Bacterial Endophytes from Root Nodules of Wild Leguminous Plant Tephrosia Purpurea (L) Pers.
title_fullStr PGP Attributes of Non-rhizobial Bacterial Endophytes from Root Nodules of Wild Leguminous Plant Tephrosia Purpurea (L) Pers.
title_full_unstemmed PGP Attributes of Non-rhizobial Bacterial Endophytes from Root Nodules of Wild Leguminous Plant Tephrosia Purpurea (L) Pers.
title_short PGP Attributes of Non-rhizobial Bacterial Endophytes from Root Nodules of Wild Leguminous Plant Tephrosia Purpurea (L) Pers.
title_sort pgp attributes of non rhizobial bacterial endophytes from root nodules of wild leguminous plant tephrosia purpurea l pers
topic Wild Legumes
PGPR
Biochemical Test
Vegetative Growth
Pot Trial
url https://journal.environcj.in/index.php/ecj/article/view/2759
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