First Attempts at DNA Barcoding Lepidoptera in North Cyprus Reveal Unexpected Complexities in Taxonomic and Faunistic Issues
The fauna of Lepidoptera in the Mediterranean is still inadequately documented. As a result, even remotely complete DNA barcode libraries (mt. COI (cytochrome c oxidase 1) gene) are lacking in most areas. This proposed gap is being analyzed for the first time for the fauna of North Cyprus. In the in...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Diversity |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-2818/16/11/671 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846153744169828352 |
|---|---|
| author | Peter Huemer Özge Özden |
| author_facet | Peter Huemer Özge Özden |
| author_sort | Peter Huemer |
| collection | DOAJ |
| description | The fauna of Lepidoptera in the Mediterranean is still inadequately documented. As a result, even remotely complete DNA barcode libraries (mt. COI (cytochrome c oxidase 1) gene) are lacking in most areas. This proposed gap is being analyzed for the first time for the fauna of North Cyprus. In the initial phase, 248 morphospecies from 29 families (exclusive Heterocera) were sampled, sequenced and compared with existing DNA reference sequences in the global BOLD database (Barcode of Life Data Systems) via BINs (Barcode Index Numbers). A total of 194 species could be unequivocally assigned to a Linnaean taxon. Additionally, six species previously unidentified in BOLD, as well as fourteen species without reference barcodes, were identified at the species level. Twenty-four of these species were new records for Cyprus. In addition, 25 taxa with new BINs could not be assigned to a valid species due to potential cryptic diversity or the lack of relevant revisions. Furthermore, a few species could not be identified due to barcode sharing and/or potential misidentifications in BOLD. Overall, approximately 20% of the samples could not be identified using the existing DNA barcode libraries, a significant deficit for European standards, which should be addressed as a priority issue in future studies. |
| format | Article |
| id | doaj-art-a49a072a40644123b23e34e8f0fbb5ef |
| institution | Kabale University |
| issn | 1424-2818 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj-art-a49a072a40644123b23e34e8f0fbb5ef2024-11-26T18:00:22ZengMDPI AGDiversity1424-28182024-10-01161167110.3390/d16110671First Attempts at DNA Barcoding Lepidoptera in North Cyprus Reveal Unexpected Complexities in Taxonomic and Faunistic IssuesPeter Huemer0Özge Özden1Naturwissenschaftliche Sammlungen, Sammlungs- und Forschungszentrum, Tiroler Landesmuseen Betriebsges.m.b.H., 6060 Hall in Tirol, AustriaFaculty of Agriculture, Near East University, 99138 Nicosia, CyprusThe fauna of Lepidoptera in the Mediterranean is still inadequately documented. As a result, even remotely complete DNA barcode libraries (mt. COI (cytochrome c oxidase 1) gene) are lacking in most areas. This proposed gap is being analyzed for the first time for the fauna of North Cyprus. In the initial phase, 248 morphospecies from 29 families (exclusive Heterocera) were sampled, sequenced and compared with existing DNA reference sequences in the global BOLD database (Barcode of Life Data Systems) via BINs (Barcode Index Numbers). A total of 194 species could be unequivocally assigned to a Linnaean taxon. Additionally, six species previously unidentified in BOLD, as well as fourteen species without reference barcodes, were identified at the species level. Twenty-four of these species were new records for Cyprus. In addition, 25 taxa with new BINs could not be assigned to a valid species due to potential cryptic diversity or the lack of relevant revisions. Furthermore, a few species could not be identified due to barcode sharing and/or potential misidentifications in BOLD. Overall, approximately 20% of the samples could not be identified using the existing DNA barcode libraries, a significant deficit for European standards, which should be addressed as a priority issue in future studies.https://www.mdpi.com/1424-2818/16/11/671barcode index number (BIN)DNA barcodingendemismfaunisticsislandMediterranean |
| spellingShingle | Peter Huemer Özge Özden First Attempts at DNA Barcoding Lepidoptera in North Cyprus Reveal Unexpected Complexities in Taxonomic and Faunistic Issues Diversity barcode index number (BIN) DNA barcoding endemism faunistics island Mediterranean |
| title | First Attempts at DNA Barcoding Lepidoptera in North Cyprus Reveal Unexpected Complexities in Taxonomic and Faunistic Issues |
| title_full | First Attempts at DNA Barcoding Lepidoptera in North Cyprus Reveal Unexpected Complexities in Taxonomic and Faunistic Issues |
| title_fullStr | First Attempts at DNA Barcoding Lepidoptera in North Cyprus Reveal Unexpected Complexities in Taxonomic and Faunistic Issues |
| title_full_unstemmed | First Attempts at DNA Barcoding Lepidoptera in North Cyprus Reveal Unexpected Complexities in Taxonomic and Faunistic Issues |
| title_short | First Attempts at DNA Barcoding Lepidoptera in North Cyprus Reveal Unexpected Complexities in Taxonomic and Faunistic Issues |
| title_sort | first attempts at dna barcoding lepidoptera in north cyprus reveal unexpected complexities in taxonomic and faunistic issues |
| topic | barcode index number (BIN) DNA barcoding endemism faunistics island Mediterranean |
| url | https://www.mdpi.com/1424-2818/16/11/671 |
| work_keys_str_mv | AT peterhuemer firstattemptsatdnabarcodinglepidopterainnorthcyprusrevealunexpectedcomplexitiesintaxonomicandfaunisticissues AT ozgeozden firstattemptsatdnabarcodinglepidopterainnorthcyprusrevealunexpectedcomplexitiesintaxonomicandfaunisticissues |