Circular RNA WRNIP1 activates the PI3K-AKT and ERK1/2 signaling pathways by binding to miR-129-5p/IGF2 axis and facilitates ovarian follicle development in chickens
The healthy and orderly development of ovarian follicles is the basic premise to maintain normal and continuous egg production of chickens. Emerging researches continue to reveal the crucial functions of circular RNAs (circRNAs) involved in follicle development. In the present study, a novel circula...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S003257912401335X |
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| author | Xiyu Zhao Yuanhang Wei Xinyan Li Xinghong Yu Zhenyu Lei Yao Zhang Shunshun Han Huadong Yin Can Cui |
| author_facet | Xiyu Zhao Yuanhang Wei Xinyan Li Xinghong Yu Zhenyu Lei Yao Zhang Shunshun Han Huadong Yin Can Cui |
| author_sort | Xiyu Zhao |
| collection | DOAJ |
| description | The healthy and orderly development of ovarian follicles is the basic premise to maintain normal and continuous egg production of chickens. Emerging researches continue to reveal the crucial functions of circular RNAs (circRNAs) involved in follicle development. In the present study, a novel circular RNA WRNIP1 (circWRNIP1) with higher abundance in healthy follicles than in atretic follicles was identified, suggesting its important regulatory role in follicle selection and maturation. In this study, we demonstrate that circWRNIP1 promotes chicken ovarian follicle development by enhancing granulosa cell proliferation and inhibiting apoptosis. Furthermore, we validated the targeting relationships between circWRNIP1 and miR-129-5p, as well as between miR-129-5p and IGF2. Mechanistically, through co-transfection experiments, we show that circWRNIP1 activates the PI3K-AKT and ERK1/2 signaling pathways via the miR-129-5p/IGF2 axis. The present study provides new insights into the downstream molecular mechanism of circRNAs regulating follicle development, and is expected to provide reliable targets for the subsequent establishment of new molecular breeding strategies. |
| format | Article |
| id | doaj-art-a48db200965f4fe1a0f6eef3e9d31fe9 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-a48db200965f4fe1a0f6eef3e9d31fe92025-01-05T04:27:45ZengElsevierPoultry Science0032-57912025-02-011042104757Circular RNA WRNIP1 activates the PI3K-AKT and ERK1/2 signaling pathways by binding to miR-129-5p/IGF2 axis and facilitates ovarian follicle development in chickensXiyu Zhao0Yuanhang Wei1Xinyan Li2Xinghong Yu3Zhenyu Lei4Yao Zhang5Shunshun Han6Huadong Yin7Can Cui8Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR ChinaKey Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR ChinaKey Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR ChinaKey Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR ChinaKey Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR ChinaKey Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR ChinaKey Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR ChinaKey Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China; Corresponding author.Key Laboratory of Livestock and Poultry Multi-omics, Ministry of Agriculture and Rural Affairs, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR China; Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, PR ChinaThe healthy and orderly development of ovarian follicles is the basic premise to maintain normal and continuous egg production of chickens. Emerging researches continue to reveal the crucial functions of circular RNAs (circRNAs) involved in follicle development. In the present study, a novel circular RNA WRNIP1 (circWRNIP1) with higher abundance in healthy follicles than in atretic follicles was identified, suggesting its important regulatory role in follicle selection and maturation. In this study, we demonstrate that circWRNIP1 promotes chicken ovarian follicle development by enhancing granulosa cell proliferation and inhibiting apoptosis. Furthermore, we validated the targeting relationships between circWRNIP1 and miR-129-5p, as well as between miR-129-5p and IGF2. Mechanistically, through co-transfection experiments, we show that circWRNIP1 activates the PI3K-AKT and ERK1/2 signaling pathways via the miR-129-5p/IGF2 axis. The present study provides new insights into the downstream molecular mechanism of circRNAs regulating follicle development, and is expected to provide reliable targets for the subsequent establishment of new molecular breeding strategies.http://www.sciencedirect.com/science/article/pii/S003257912401335XFollicle developmentcircWRNIP1Granulosa cellsProliferationApoptosis |
| spellingShingle | Xiyu Zhao Yuanhang Wei Xinyan Li Xinghong Yu Zhenyu Lei Yao Zhang Shunshun Han Huadong Yin Can Cui Circular RNA WRNIP1 activates the PI3K-AKT and ERK1/2 signaling pathways by binding to miR-129-5p/IGF2 axis and facilitates ovarian follicle development in chickens Poultry Science Follicle development circWRNIP1 Granulosa cells Proliferation Apoptosis |
| title | Circular RNA WRNIP1 activates the PI3K-AKT and ERK1/2 signaling pathways by binding to miR-129-5p/IGF2 axis and facilitates ovarian follicle development in chickens |
| title_full | Circular RNA WRNIP1 activates the PI3K-AKT and ERK1/2 signaling pathways by binding to miR-129-5p/IGF2 axis and facilitates ovarian follicle development in chickens |
| title_fullStr | Circular RNA WRNIP1 activates the PI3K-AKT and ERK1/2 signaling pathways by binding to miR-129-5p/IGF2 axis and facilitates ovarian follicle development in chickens |
| title_full_unstemmed | Circular RNA WRNIP1 activates the PI3K-AKT and ERK1/2 signaling pathways by binding to miR-129-5p/IGF2 axis and facilitates ovarian follicle development in chickens |
| title_short | Circular RNA WRNIP1 activates the PI3K-AKT and ERK1/2 signaling pathways by binding to miR-129-5p/IGF2 axis and facilitates ovarian follicle development in chickens |
| title_sort | circular rna wrnip1 activates the pi3k akt and erk1 2 signaling pathways by binding to mir 129 5p igf2 axis and facilitates ovarian follicle development in chickens |
| topic | Follicle development circWRNIP1 Granulosa cells Proliferation Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S003257912401335X |
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