Exosomes derived from platelet-rich plasma present a novel potential in repairing knee articular cartilage defect combined with cyclic peptide-modified β-TCP scaffold
Abstract Background The aim of this study was to investigate the therapeutic effects and mechanisms of PRP-exos combined with cyclic peptide-modified β-TCP scaffold in the treatment of rabbit knee cartilage defect. Methods PRP-exos were extracted and characterized by TEM, NTA and WB. The therapeutic...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-11-01
|
| Series: | Journal of Orthopaedic Surgery and Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13018-024-05202-z |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846171758797783040 |
|---|---|
| author | Xuchang Liu Rudong Chen Guanzheng Cui Rongjie Feng Kechun Liu |
| author_facet | Xuchang Liu Rudong Chen Guanzheng Cui Rongjie Feng Kechun Liu |
| author_sort | Xuchang Liu |
| collection | DOAJ |
| description | Abstract Background The aim of this study was to investigate the therapeutic effects and mechanisms of PRP-exos combined with cyclic peptide-modified β-TCP scaffold in the treatment of rabbit knee cartilage defect. Methods PRP-exos were extracted and characterized by TEM, NTA and WB. The therapeutic effects were evaluated by ICRS score, HE staining, Immunohistochemistry, qRT-PCR and ELISA. The repair mechanism of PRP-exos was estimated and predicted by miRNA sequencing analysis and protein–protein interaction network analysis. Results The results showed that PRP-exos had a reasonable size distribution and exhibited typical exosome morphology. The combination of PRP-exos and cyclic peptide-modified β-TCP scaffold improved ICRS score and the expression level of COL-2, RUNX2, and SOX9. Moreover, this combination therapy reduced the level of MMP-3, TNF-α, IL-1β, and IL-6, while increasing the level of TIMP-1. In PRP-exos miRNA sequencing analysis, the total number of known miRNAs aligned across all samples was 252, and a total of 91 differentially expressed miRNAs were detected. The results of KEGG enrichment analysis and the protein–protein interaction network analysis indicated that the PI3K/AKT signaling pathway could impact the function of chondrocytes by regulating key transcription factors to repair cartilage defect. Conclusion PRP-exos combined with cyclic peptide-modified β-TCP scaffold effectively promoted cartilage repair and improved chondrocyte function in rabbit knee cartilage defect. Based on the analysis and prediction of PRP-exos miRNAs sequencing, PI3K/AKT signaling pathway may contribute to the therapeutic effect. These findings provide experimental evidence for the application of PRP-exos in the treatment of cartilage defect. |
| format | Article |
| id | doaj-art-a47e08d04da346c5a07306952e1f7a05 |
| institution | Kabale University |
| issn | 1749-799X |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Orthopaedic Surgery and Research |
| spelling | doaj-art-a47e08d04da346c5a07306952e1f7a052024-11-10T12:34:49ZengBMCJournal of Orthopaedic Surgery and Research1749-799X2024-11-0119111710.1186/s13018-024-05202-zExosomes derived from platelet-rich plasma present a novel potential in repairing knee articular cartilage defect combined with cyclic peptide-modified β-TCP scaffoldXuchang Liu0Rudong Chen1Guanzheng Cui2Rongjie Feng3Kechun Liu4Department of Orthopedic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityDepartment of Orthopedic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityDepartment of Orthopedic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityDepartment of Orthopedic Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityBiology Institute, Qilu University of Technology (Shandong Academy of Sciences)Abstract Background The aim of this study was to investigate the therapeutic effects and mechanisms of PRP-exos combined with cyclic peptide-modified β-TCP scaffold in the treatment of rabbit knee cartilage defect. Methods PRP-exos were extracted and characterized by TEM, NTA and WB. The therapeutic effects were evaluated by ICRS score, HE staining, Immunohistochemistry, qRT-PCR and ELISA. The repair mechanism of PRP-exos was estimated and predicted by miRNA sequencing analysis and protein–protein interaction network analysis. Results The results showed that PRP-exos had a reasonable size distribution and exhibited typical exosome morphology. The combination of PRP-exos and cyclic peptide-modified β-TCP scaffold improved ICRS score and the expression level of COL-2, RUNX2, and SOX9. Moreover, this combination therapy reduced the level of MMP-3, TNF-α, IL-1β, and IL-6, while increasing the level of TIMP-1. In PRP-exos miRNA sequencing analysis, the total number of known miRNAs aligned across all samples was 252, and a total of 91 differentially expressed miRNAs were detected. The results of KEGG enrichment analysis and the protein–protein interaction network analysis indicated that the PI3K/AKT signaling pathway could impact the function of chondrocytes by regulating key transcription factors to repair cartilage defect. Conclusion PRP-exos combined with cyclic peptide-modified β-TCP scaffold effectively promoted cartilage repair and improved chondrocyte function in rabbit knee cartilage defect. Based on the analysis and prediction of PRP-exos miRNAs sequencing, PI3K/AKT signaling pathway may contribute to the therapeutic effect. These findings provide experimental evidence for the application of PRP-exos in the treatment of cartilage defect.https://doi.org/10.1186/s13018-024-05202-zPRP-exosCyclic peptideβ-TCP scaffoldCartilage defectPI3K/AKT signaling pathway |
| spellingShingle | Xuchang Liu Rudong Chen Guanzheng Cui Rongjie Feng Kechun Liu Exosomes derived from platelet-rich plasma present a novel potential in repairing knee articular cartilage defect combined with cyclic peptide-modified β-TCP scaffold Journal of Orthopaedic Surgery and Research PRP-exos Cyclic peptide β-TCP scaffold Cartilage defect PI3K/AKT signaling pathway |
| title | Exosomes derived from platelet-rich plasma present a novel potential in repairing knee articular cartilage defect combined with cyclic peptide-modified β-TCP scaffold |
| title_full | Exosomes derived from platelet-rich plasma present a novel potential in repairing knee articular cartilage defect combined with cyclic peptide-modified β-TCP scaffold |
| title_fullStr | Exosomes derived from platelet-rich plasma present a novel potential in repairing knee articular cartilage defect combined with cyclic peptide-modified β-TCP scaffold |
| title_full_unstemmed | Exosomes derived from platelet-rich plasma present a novel potential in repairing knee articular cartilage defect combined with cyclic peptide-modified β-TCP scaffold |
| title_short | Exosomes derived from platelet-rich plasma present a novel potential in repairing knee articular cartilage defect combined with cyclic peptide-modified β-TCP scaffold |
| title_sort | exosomes derived from platelet rich plasma present a novel potential in repairing knee articular cartilage defect combined with cyclic peptide modified β tcp scaffold |
| topic | PRP-exos Cyclic peptide β-TCP scaffold Cartilage defect PI3K/AKT signaling pathway |
| url | https://doi.org/10.1186/s13018-024-05202-z |
| work_keys_str_mv | AT xuchangliu exosomesderivedfromplateletrichplasmapresentanovelpotentialinrepairingkneearticularcartilagedefectcombinedwithcyclicpeptidemodifiedbtcpscaffold AT rudongchen exosomesderivedfromplateletrichplasmapresentanovelpotentialinrepairingkneearticularcartilagedefectcombinedwithcyclicpeptidemodifiedbtcpscaffold AT guanzhengcui exosomesderivedfromplateletrichplasmapresentanovelpotentialinrepairingkneearticularcartilagedefectcombinedwithcyclicpeptidemodifiedbtcpscaffold AT rongjiefeng exosomesderivedfromplateletrichplasmapresentanovelpotentialinrepairingkneearticularcartilagedefectcombinedwithcyclicpeptidemodifiedbtcpscaffold AT kechunliu exosomesderivedfromplateletrichplasmapresentanovelpotentialinrepairingkneearticularcartilagedefectcombinedwithcyclicpeptidemodifiedbtcpscaffold |