Spectrum of Isolates and Antimicrobial Susceptibility Pattern from Blood Cultures in Tertiary Care Hospital, Riyadh

Clinical presentation of bloodstream infection ranges from transitory bacteremia to fulminant septic shock with substantial mortality. Regular monitoring of bacteremia etiology helps rationalize therapy, revealing the spectrum of bacterial infections and their sensitivity pattern in a particular are...

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Main Authors: Fizza Khalid, Christy Poulose, Azza Elsheikh, Abid Mahmood, Osamah T. Khojah
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2025-06-01
Series:Journal of Pure and Applied Microbiology
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Online Access:https://microbiologyjournal.org/spectrum-of-isolates-and-antimicrobial-susceptibility-pattern-from-blood-cultures-in-tertiary-care-hospital-riyadh/
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author Fizza Khalid
Christy Poulose
Azza Elsheikh
Abid Mahmood
Osamah T. Khojah
author_facet Fizza Khalid
Christy Poulose
Azza Elsheikh
Abid Mahmood
Osamah T. Khojah
author_sort Fizza Khalid
collection DOAJ
description Clinical presentation of bloodstream infection ranges from transitory bacteremia to fulminant septic shock with substantial mortality. Regular monitoring of bacteremia etiology helps rationalize therapy, revealing the spectrum of bacterial infections and their sensitivity pattern in a particular area. In this study, we investigated the prevalence and antimicrobial susceptibility profile of all bacterial pathogens isolated from blood cultures in a tertiary care hospital in Riyadh, Saudi Arabia. It was a retrospective study and data was collected over a period of one year from January 2022 to December 2022, with a total of 3,429 blood cultures requested. Culture positivity was found in 5% of suspected bacteremia cases, with a slight male predominance. Gram-positive bacteremia was 57%, mainly isolated from male patients. Methicillin-resistant Staphylococcus aureus (MRSA) was found in 38% of total Staphylococcus aureus (S. aureus) bacteremia cases, with all Gram-positive isolates susceptible to vancomycin and linezolid. Escherichia coli (E. coli) was the most common Gram-negative bacteria, while Pseudomonas and Stenotrophomonas species were the main non-fermenting pathogens, accounting for 66.7% of cases. Extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae accounts for 17.5% in total with the highest production in Proteus species, whereas 75% of Pseudomonas species were carbapenamase producers. Consequently, the prevalence of multidrug-resistance microorganisms in critically ill individuals may account as a significant threat in hospital settings. Continuous monitoring is essential for comprehending the prevalence of bacteremia and their susceptibility pattern to create successful empirical therapy options and antimicrobial stewardship.
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spelling doaj-art-a45fe9a85d3c4b4d958e6e954772ec4f2025-08-20T03:45:38ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2025-06-011929981009https://doi.org/10.22207/JPAM.19.2.03Spectrum of Isolates and Antimicrobial Susceptibility Pattern from Blood Cultures in Tertiary Care Hospital, RiyadhFizza Khalidhttps://orcid.org/0009-0002-8975-0205Christy PouloseAzza ElsheikhAbid MahmoodOsamah T. Khojahhttps://orcid.org/0009-0002-8975-0205Clinical presentation of bloodstream infection ranges from transitory bacteremia to fulminant septic shock with substantial mortality. Regular monitoring of bacteremia etiology helps rationalize therapy, revealing the spectrum of bacterial infections and their sensitivity pattern in a particular area. In this study, we investigated the prevalence and antimicrobial susceptibility profile of all bacterial pathogens isolated from blood cultures in a tertiary care hospital in Riyadh, Saudi Arabia. It was a retrospective study and data was collected over a period of one year from January 2022 to December 2022, with a total of 3,429 blood cultures requested. Culture positivity was found in 5% of suspected bacteremia cases, with a slight male predominance. Gram-positive bacteremia was 57%, mainly isolated from male patients. Methicillin-resistant Staphylococcus aureus (MRSA) was found in 38% of total Staphylococcus aureus (S. aureus) bacteremia cases, with all Gram-positive isolates susceptible to vancomycin and linezolid. Escherichia coli (E. coli) was the most common Gram-negative bacteria, while Pseudomonas and Stenotrophomonas species were the main non-fermenting pathogens, accounting for 66.7% of cases. Extended-spectrum beta-lactamase (ESBL) producing Enterobacteriaceae accounts for 17.5% in total with the highest production in Proteus species, whereas 75% of Pseudomonas species were carbapenamase producers. Consequently, the prevalence of multidrug-resistance microorganisms in critically ill individuals may account as a significant threat in hospital settings. Continuous monitoring is essential for comprehending the prevalence of bacteremia and their susceptibility pattern to create successful empirical therapy options and antimicrobial stewardship.https://microbiologyjournal.org/spectrum-of-isolates-and-antimicrobial-susceptibility-pattern-from-blood-cultures-in-tertiary-care-hospital-riyadh/antimicrobial susceptibilitybacterial pathogensbacteremiablood culturemethicillin-resistant staphylococcus aureus
spellingShingle Fizza Khalid
Christy Poulose
Azza Elsheikh
Abid Mahmood
Osamah T. Khojah
Spectrum of Isolates and Antimicrobial Susceptibility Pattern from Blood Cultures in Tertiary Care Hospital, Riyadh
Journal of Pure and Applied Microbiology
antimicrobial susceptibility
bacterial pathogens
bacteremia
blood culture
methicillin-resistant staphylococcus aureus
title Spectrum of Isolates and Antimicrobial Susceptibility Pattern from Blood Cultures in Tertiary Care Hospital, Riyadh
title_full Spectrum of Isolates and Antimicrobial Susceptibility Pattern from Blood Cultures in Tertiary Care Hospital, Riyadh
title_fullStr Spectrum of Isolates and Antimicrobial Susceptibility Pattern from Blood Cultures in Tertiary Care Hospital, Riyadh
title_full_unstemmed Spectrum of Isolates and Antimicrobial Susceptibility Pattern from Blood Cultures in Tertiary Care Hospital, Riyadh
title_short Spectrum of Isolates and Antimicrobial Susceptibility Pattern from Blood Cultures in Tertiary Care Hospital, Riyadh
title_sort spectrum of isolates and antimicrobial susceptibility pattern from blood cultures in tertiary care hospital riyadh
topic antimicrobial susceptibility
bacterial pathogens
bacteremia
blood culture
methicillin-resistant staphylococcus aureus
url https://microbiologyjournal.org/spectrum-of-isolates-and-antimicrobial-susceptibility-pattern-from-blood-cultures-in-tertiary-care-hospital-riyadh/
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