Temporal Changes in Faecal Microbiota Composition and Diversity in Dairy Cows Supplemented with a <i>Lactobacillus</i>-Based Direct-Fed Microbial
The rumen microbiota of dairy cows plays a crucial role in fermenting fibrous material, essential for nutrient extraction and overall productivity, detoxification of anti-nutritional toxic compounds, synthesis of vital nutrients, and is essential for optimal animal health. This study investigated th...
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2024-11-01
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| author | Bronwyn E. Campbell Mohammad Mahmudul Hassan Robert J. Moore Timothy Olchowy Shahab Ranjbar Martin Soust Orlando Ramirez-Garzon Rafat Al Jassim John I. Alawneh |
| author_facet | Bronwyn E. Campbell Mohammad Mahmudul Hassan Robert J. Moore Timothy Olchowy Shahab Ranjbar Martin Soust Orlando Ramirez-Garzon Rafat Al Jassim John I. Alawneh |
| author_sort | Bronwyn E. Campbell |
| collection | DOAJ |
| description | The rumen microbiota of dairy cows plays a crucial role in fermenting fibrous material, essential for nutrient extraction and overall productivity, detoxification of anti-nutritional toxic compounds, synthesis of vital nutrients, and is essential for optimal animal health. This study investigated the impact of <i>Lentilactobacillus</i>-, <i>Lactocaseibacillus</i>-, and <i>Lacticaseibacillus</i>-based direct-fed microbial (DFM) supplementation on dairy cows’ faecal microbial composition and diversity. The study was carried out on a commercial dairy farm using 50 Holstein-Friesian cows randomly assigned into control (CON) and treatment (TRT) groups. Faecal samples were collected directly from the rectum every two months from September 2021 to January 2023. The bacterial 16S rRNA gene and fungal ITS-1 regions were amplified, sequenced, and analysed. Microbial diversity was assessed through alpha- and beta-diversity metrics. Linear discriminant analysis effect size (LEfSe) was performed to identify which taxa were driving the changes seen in the microbiota over time and treatment. <i>Bacteroidaceae</i> were the most prevalent bacterial family, followed by <i>Lachnospiraceae</i> and <i>Muribaculaceae</i> in both CON and TRT cows. <i>Ascomycota</i>, <i>Basidiomycota</i>, and <i>Mucoromycota</i> were the dominant three fungal phyla in the faeces of both CON and TRT cows. Bacterial genera <i>Fructilactobacillus</i> was abundant in the CON and <i>Absicoccus</i> in the TRT groups. Fungal taxa <i>Chaetothryriales</i>_incertae_sedis and <i>Pseudomentella</i> were absent in the faeces of TRT cows. Significant temporal and specific taxonomic differences were observed between the CON and TRT groups. The study’s findings underscore the dynamic nature of microbial communities and the importance of targeted dietary interventions. Further research is necessary to elucidate these microbial shifts, long-term impacts, and functional implications, aiming to optimise ruminant nutrition and enhance dairy cow performance. |
| format | Article |
| id | doaj-art-a4027f9d3fbe4d56abdf908513f2334e |
| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-11-01 |
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| spelling | doaj-art-a4027f9d3fbe4d56abdf908513f2334e2024-12-13T16:21:12ZengMDPI AGAnimals2076-26152024-11-011423343710.3390/ani14233437Temporal Changes in Faecal Microbiota Composition and Diversity in Dairy Cows Supplemented with a <i>Lactobacillus</i>-Based Direct-Fed MicrobialBronwyn E. Campbell0Mohammad Mahmudul Hassan1Robert J. Moore2Timothy Olchowy3Shahab Ranjbar4Martin Soust5Orlando Ramirez-Garzon6Rafat Al Jassim7John I. Alawneh8School of Science, RMIT University, Bundoora, Melbourne, VIC 3068, AustraliaSchool of Veterinary Science, The University of Queensland, Gatton, QLD 4343, AustraliaSchool of Science, RMIT University, Bundoora, Melbourne, VIC 3068, AustraliaFaculty of Veterinary Medicine, University of Calgary, Calgary, AB T3R 1J3, CanadaSchool of Veterinary Science, The University of Queensland, Gatton, QLD 4343, AustraliaTerragen Biotech Pty Ltd., Coolum Beach, QLD 4573, AustraliaTerragen Biotech Pty Ltd., Coolum Beach, QLD 4573, AustraliaQueensland Alliance for Agriculture and Food Innovation, Brisbane, QLD 4072, AustraliaSchool of Veterinary Science, The University of Queensland, Gatton, QLD 4343, AustraliaThe rumen microbiota of dairy cows plays a crucial role in fermenting fibrous material, essential for nutrient extraction and overall productivity, detoxification of anti-nutritional toxic compounds, synthesis of vital nutrients, and is essential for optimal animal health. This study investigated the impact of <i>Lentilactobacillus</i>-, <i>Lactocaseibacillus</i>-, and <i>Lacticaseibacillus</i>-based direct-fed microbial (DFM) supplementation on dairy cows’ faecal microbial composition and diversity. The study was carried out on a commercial dairy farm using 50 Holstein-Friesian cows randomly assigned into control (CON) and treatment (TRT) groups. Faecal samples were collected directly from the rectum every two months from September 2021 to January 2023. The bacterial 16S rRNA gene and fungal ITS-1 regions were amplified, sequenced, and analysed. Microbial diversity was assessed through alpha- and beta-diversity metrics. Linear discriminant analysis effect size (LEfSe) was performed to identify which taxa were driving the changes seen in the microbiota over time and treatment. <i>Bacteroidaceae</i> were the most prevalent bacterial family, followed by <i>Lachnospiraceae</i> and <i>Muribaculaceae</i> in both CON and TRT cows. <i>Ascomycota</i>, <i>Basidiomycota</i>, and <i>Mucoromycota</i> were the dominant three fungal phyla in the faeces of both CON and TRT cows. Bacterial genera <i>Fructilactobacillus</i> was abundant in the CON and <i>Absicoccus</i> in the TRT groups. Fungal taxa <i>Chaetothryriales</i>_incertae_sedis and <i>Pseudomentella</i> were absent in the faeces of TRT cows. Significant temporal and specific taxonomic differences were observed between the CON and TRT groups. The study’s findings underscore the dynamic nature of microbial communities and the importance of targeted dietary interventions. Further research is necessary to elucidate these microbial shifts, long-term impacts, and functional implications, aiming to optimise ruminant nutrition and enhance dairy cow performance.https://www.mdpi.com/2076-2615/14/23/3437faecal microbiotadairy cowsDFMmicrobial diversitytemporal changes |
| spellingShingle | Bronwyn E. Campbell Mohammad Mahmudul Hassan Robert J. Moore Timothy Olchowy Shahab Ranjbar Martin Soust Orlando Ramirez-Garzon Rafat Al Jassim John I. Alawneh Temporal Changes in Faecal Microbiota Composition and Diversity in Dairy Cows Supplemented with a <i>Lactobacillus</i>-Based Direct-Fed Microbial Animals faecal microbiota dairy cows DFM microbial diversity temporal changes |
| title | Temporal Changes in Faecal Microbiota Composition and Diversity in Dairy Cows Supplemented with a <i>Lactobacillus</i>-Based Direct-Fed Microbial |
| title_full | Temporal Changes in Faecal Microbiota Composition and Diversity in Dairy Cows Supplemented with a <i>Lactobacillus</i>-Based Direct-Fed Microbial |
| title_fullStr | Temporal Changes in Faecal Microbiota Composition and Diversity in Dairy Cows Supplemented with a <i>Lactobacillus</i>-Based Direct-Fed Microbial |
| title_full_unstemmed | Temporal Changes in Faecal Microbiota Composition and Diversity in Dairy Cows Supplemented with a <i>Lactobacillus</i>-Based Direct-Fed Microbial |
| title_short | Temporal Changes in Faecal Microbiota Composition and Diversity in Dairy Cows Supplemented with a <i>Lactobacillus</i>-Based Direct-Fed Microbial |
| title_sort | temporal changes in faecal microbiota composition and diversity in dairy cows supplemented with a i lactobacillus i based direct fed microbial |
| topic | faecal microbiota dairy cows DFM microbial diversity temporal changes |
| url | https://www.mdpi.com/2076-2615/14/23/3437 |
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