Acute Effects of Photobiomodulation Therapy Combined with Static Magnetic Field in C2C12 Muscle Cells Exposed and Not Exposed to H<sub>2</sub>O<sub>2</sub>
Photobiomodulation (PBM) has been applied in biomedical technology to improve cellular responses. Specifically in sports medicine, it is used to accelerate metabolic and structural repair and adaptation in skeletal muscle under stress overload. Currently, PBM has been associated with static Magnetic...
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2024-11-01
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| author | Marcos Vinicius Ferlito João Vitor Ferlito Fernando Joel Scariot Mirian Salvador Ernesto Cesar Pinto Leal-Junior Thiago De Marchi Catia Santos Branco |
| author_facet | Marcos Vinicius Ferlito João Vitor Ferlito Fernando Joel Scariot Mirian Salvador Ernesto Cesar Pinto Leal-Junior Thiago De Marchi Catia Santos Branco |
| author_sort | Marcos Vinicius Ferlito |
| collection | DOAJ |
| description | Photobiomodulation (PBM) has been applied in biomedical technology to improve cellular responses. Specifically in sports medicine, it is used to accelerate metabolic and structural repair and adaptation in skeletal muscle under stress overload. Currently, PBM has been associated with static Magnetic Field (sMF) in clinical applications, enhancing the effects displayed by PBM when used in isolation. However, the biochemical and molecular effects of PBM-sMF in myoblasts remain unknown. This study aimed to investigate the effects of PBM combined with static magnetic field (PBM-sMF) at different doses in C2C12 muscle cells in the presence or absence of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), a standard oxidant. Different spectrophotometric and fluorometric assays were conducted after cellular treatments. PBM-sMF was shown to be effective compared to H<sub>2</sub>O<sub>2</sub> regarding cell viability and release of nitric oxide (NO), dsDNA, and reactive oxygen species (ROS) levels. It positively modulated mitochondrial membrane potential (ΔΨm) and cytochrome c oxidase (COX) activity under normal conditions and restored both to normal levels when impacted by H<sub>2</sub>O<sub>2</sub>. Regarding apoptosis, the recovery in viable cells observed on PBM-sMF treated cells was dose-dependent. In conclusion, PBM-sMF has a biphasic effect in normal and oxidative environments and may differently modulate myoblast cells depending on their redox status. |
| format | Article |
| id | doaj-art-a3f6d3b52d7d45699b64bb28e3ba0131 |
| institution | Kabale University |
| issn | 2076-3417 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| spelling | doaj-art-a3f6d3b52d7d45699b64bb28e3ba01312024-11-26T17:47:57ZengMDPI AGApplied Sciences2076-34172024-11-0114221022110.3390/app142210221Acute Effects of Photobiomodulation Therapy Combined with Static Magnetic Field in C2C12 Muscle Cells Exposed and Not Exposed to H<sub>2</sub>O<sub>2</sub>Marcos Vinicius Ferlito0João Vitor Ferlito1Fernando Joel Scariot2Mirian Salvador3Ernesto Cesar Pinto Leal-Junior4Thiago De Marchi5Catia Santos Branco6Laboratory of Oxidative Stress and Antioxidants, Institute of Biotechnology, University of Caxias Do Sul (UCS), Caxias do Sul 95070-560, RS, BrazilLaboratory of Oxidative Stress and Antioxidants, Institute of Biotechnology, University of Caxias Do Sul (UCS), Caxias do Sul 95070-560, RS, BrazilLaboratory of Applied Microbiology, Institute of Biotechnology, University of Caxias do Sul (UCS), Caxias do Sul 95070-560, RS, BrazilLaboratory of Oxidative Stress and Antioxidants, Institute of Biotechnology, University of Caxias Do Sul (UCS), Caxias do Sul 95070-560, RS, BrazilLaboratory of Phototherapy and Innovative Technologies in Health (LaPIT), Nove de Julho University (UNINOVE), São Paulo 01525-000, SP, BrazilPhysiotherapy Course, UNIFTEC University Center, Bento Gonçalves 95700-000, RS, BrazilLaboratory of Oxidative Stress and Antioxidants, Institute of Biotechnology, University of Caxias Do Sul (UCS), Caxias do Sul 95070-560, RS, BrazilPhotobiomodulation (PBM) has been applied in biomedical technology to improve cellular responses. Specifically in sports medicine, it is used to accelerate metabolic and structural repair and adaptation in skeletal muscle under stress overload. Currently, PBM has been associated with static Magnetic Field (sMF) in clinical applications, enhancing the effects displayed by PBM when used in isolation. However, the biochemical and molecular effects of PBM-sMF in myoblasts remain unknown. This study aimed to investigate the effects of PBM combined with static magnetic field (PBM-sMF) at different doses in C2C12 muscle cells in the presence or absence of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), a standard oxidant. Different spectrophotometric and fluorometric assays were conducted after cellular treatments. PBM-sMF was shown to be effective compared to H<sub>2</sub>O<sub>2</sub> regarding cell viability and release of nitric oxide (NO), dsDNA, and reactive oxygen species (ROS) levels. It positively modulated mitochondrial membrane potential (ΔΨm) and cytochrome c oxidase (COX) activity under normal conditions and restored both to normal levels when impacted by H<sub>2</sub>O<sub>2</sub>. Regarding apoptosis, the recovery in viable cells observed on PBM-sMF treated cells was dose-dependent. In conclusion, PBM-sMF has a biphasic effect in normal and oxidative environments and may differently modulate myoblast cells depending on their redox status.https://www.mdpi.com/2076-3417/14/22/10221biomedical engineeringmyoblastmitochondriaredox metabolismreactive oxygen speciesoxidative stress |
| spellingShingle | Marcos Vinicius Ferlito João Vitor Ferlito Fernando Joel Scariot Mirian Salvador Ernesto Cesar Pinto Leal-Junior Thiago De Marchi Catia Santos Branco Acute Effects of Photobiomodulation Therapy Combined with Static Magnetic Field in C2C12 Muscle Cells Exposed and Not Exposed to H<sub>2</sub>O<sub>2</sub> Applied Sciences biomedical engineering myoblast mitochondria redox metabolism reactive oxygen species oxidative stress |
| title | Acute Effects of Photobiomodulation Therapy Combined with Static Magnetic Field in C2C12 Muscle Cells Exposed and Not Exposed to H<sub>2</sub>O<sub>2</sub> |
| title_full | Acute Effects of Photobiomodulation Therapy Combined with Static Magnetic Field in C2C12 Muscle Cells Exposed and Not Exposed to H<sub>2</sub>O<sub>2</sub> |
| title_fullStr | Acute Effects of Photobiomodulation Therapy Combined with Static Magnetic Field in C2C12 Muscle Cells Exposed and Not Exposed to H<sub>2</sub>O<sub>2</sub> |
| title_full_unstemmed | Acute Effects of Photobiomodulation Therapy Combined with Static Magnetic Field in C2C12 Muscle Cells Exposed and Not Exposed to H<sub>2</sub>O<sub>2</sub> |
| title_short | Acute Effects of Photobiomodulation Therapy Combined with Static Magnetic Field in C2C12 Muscle Cells Exposed and Not Exposed to H<sub>2</sub>O<sub>2</sub> |
| title_sort | acute effects of photobiomodulation therapy combined with static magnetic field in c2c12 muscle cells exposed and not exposed to h sub 2 sub o sub 2 sub |
| topic | biomedical engineering myoblast mitochondria redox metabolism reactive oxygen species oxidative stress |
| url | https://www.mdpi.com/2076-3417/14/22/10221 |
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