Protective effects of avocado peel and seed extracts against UVB-damaged fibroblasts for the development of an anti-photoaging nanoemulgel
Abstract This study investigates the anti-photoaging potential of ethanolic extracts from avocado peel and seed and their application in a nanoemulgel formulation. Both extracts demonstrated high phenolic content and strong antioxidant activity, with DPPH IC50 values of 57.05 ± 1.65 µg/ml (peel) and...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-13679-9 |
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| author | Suradwadee Thungmungmee Nakuntwalai Wisidsri |
| author_facet | Suradwadee Thungmungmee Nakuntwalai Wisidsri |
| author_sort | Suradwadee Thungmungmee |
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| description | Abstract This study investigates the anti-photoaging potential of ethanolic extracts from avocado peel and seed and their application in a nanoemulgel formulation. Both extracts demonstrated high phenolic content and strong antioxidant activity, with DPPH IC50 values of 57.05 ± 1.65 µg/ml (peel) and 52.44 ± 0.32 µg/ml (seed). In UVB-irradiated human dermal fibroblasts (BJ cells), the extracts (12.5–50 µg/ml) significantly reduced intracellular ROS, decreased MMP-1 and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and restored collagen levels. A nanoemulgel was developed using the phase inversion composition (PIC) method, with avocado oil, isononyl isononanoate, Tween 60, and polyglyceryl-3 diisostearate, which resulted in nanoemulsions (NEs) with an average droplet size of 84.45 ± 5.45 nm and a zeta potential of −69.94 ± 5.66 mV. Incorporation of 0.5% Aqupec HV-505E and avocado extracts produced stable, light green gel. The resulting avocado extract-loaded nanoemulgel (ANE) demonstrated antioxidant activity, cytocompatibility with BJ cells, and stability in terms of color, pH, and viscosity. These findings reveal the anti-aging potential of avocado peel and seed extracts in preserving effectiveness within nanoemulgel for skin applications. |
| format | Article |
| id | doaj-art-a3cfbb7762c44bbfaeea90cf91f007a3 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-a3cfbb7762c44bbfaeea90cf91f007a32025-08-20T03:43:21ZengNature PortfolioScientific Reports2045-23222025-07-0115111410.1038/s41598-025-13679-9Protective effects of avocado peel and seed extracts against UVB-damaged fibroblasts for the development of an anti-photoaging nanoemulgelSuradwadee Thungmungmee0Nakuntwalai Wisidsri1Faculty of Integrative Medicine, Rajamangala University of Technology ThanyaburiFaculty of Integrative Medicine, Rajamangala University of Technology ThanyaburiAbstract This study investigates the anti-photoaging potential of ethanolic extracts from avocado peel and seed and their application in a nanoemulgel formulation. Both extracts demonstrated high phenolic content and strong antioxidant activity, with DPPH IC50 values of 57.05 ± 1.65 µg/ml (peel) and 52.44 ± 0.32 µg/ml (seed). In UVB-irradiated human dermal fibroblasts (BJ cells), the extracts (12.5–50 µg/ml) significantly reduced intracellular ROS, decreased MMP-1 and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6), and restored collagen levels. A nanoemulgel was developed using the phase inversion composition (PIC) method, with avocado oil, isononyl isononanoate, Tween 60, and polyglyceryl-3 diisostearate, which resulted in nanoemulsions (NEs) with an average droplet size of 84.45 ± 5.45 nm and a zeta potential of −69.94 ± 5.66 mV. Incorporation of 0.5% Aqupec HV-505E and avocado extracts produced stable, light green gel. The resulting avocado extract-loaded nanoemulgel (ANE) demonstrated antioxidant activity, cytocompatibility with BJ cells, and stability in terms of color, pH, and viscosity. These findings reveal the anti-aging potential of avocado peel and seed extracts in preserving effectiveness within nanoemulgel for skin applications.https://doi.org/10.1038/s41598-025-13679-9AvocadoPhotoagingAntioxidantUVBNanoemulgel |
| spellingShingle | Suradwadee Thungmungmee Nakuntwalai Wisidsri Protective effects of avocado peel and seed extracts against UVB-damaged fibroblasts for the development of an anti-photoaging nanoemulgel Scientific Reports Avocado Photoaging Antioxidant UVB Nanoemulgel |
| title | Protective effects of avocado peel and seed extracts against UVB-damaged fibroblasts for the development of an anti-photoaging nanoemulgel |
| title_full | Protective effects of avocado peel and seed extracts against UVB-damaged fibroblasts for the development of an anti-photoaging nanoemulgel |
| title_fullStr | Protective effects of avocado peel and seed extracts against UVB-damaged fibroblasts for the development of an anti-photoaging nanoemulgel |
| title_full_unstemmed | Protective effects of avocado peel and seed extracts against UVB-damaged fibroblasts for the development of an anti-photoaging nanoemulgel |
| title_short | Protective effects of avocado peel and seed extracts against UVB-damaged fibroblasts for the development of an anti-photoaging nanoemulgel |
| title_sort | protective effects of avocado peel and seed extracts against uvb damaged fibroblasts for the development of an anti photoaging nanoemulgel |
| topic | Avocado Photoaging Antioxidant UVB Nanoemulgel |
| url | https://doi.org/10.1038/s41598-025-13679-9 |
| work_keys_str_mv | AT suradwadeethungmungmee protectiveeffectsofavocadopeelandseedextractsagainstuvbdamagedfibroblastsforthedevelopmentofanantiphotoagingnanoemulgel AT nakuntwalaiwisidsri protectiveeffectsofavocadopeelandseedextractsagainstuvbdamagedfibroblastsforthedevelopmentofanantiphotoagingnanoemulgel |