Pharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus Coniothyrium chaingmaiense: KUMBMDBT-25
Abstract In this study, the endophytic fungus Coniothyrium chaingmaiense-KUMBMDBT-25 was isolated from the healthy stem of Euphorbia tirucalli, mass cultivated by submerged fermentation, and extracted using ethyl acetate as a solvent. The extract was subjected to GC-MS analysis. The synthesized Con-...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-76475-x |
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| author | Manjunatha Dadayya Megha Gowri Thippeswamy Nagaraju Shivaiah Thoyajakshi Ramasamudra Siddaraju Prakash Jayaramaiah Sowmya Hirakannavar Veeranna Thippeswamy Basaiah Shridhar N Mathad Ravikumar Hemagiri Gowda Sachin Naik Abdulaziz Abdullah AI Kheraif Sajith Vellappally |
| author_facet | Manjunatha Dadayya Megha Gowri Thippeswamy Nagaraju Shivaiah Thoyajakshi Ramasamudra Siddaraju Prakash Jayaramaiah Sowmya Hirakannavar Veeranna Thippeswamy Basaiah Shridhar N Mathad Ravikumar Hemagiri Gowda Sachin Naik Abdulaziz Abdullah AI Kheraif Sajith Vellappally |
| author_sort | Manjunatha Dadayya |
| collection | DOAJ |
| description | Abstract In this study, the endophytic fungus Coniothyrium chaingmaiense-KUMBMDBT-25 was isolated from the healthy stem of Euphorbia tirucalli, mass cultivated by submerged fermentation, and extracted using ethyl acetate as a solvent. The extract was subjected to GC-MS analysis. The synthesized Con-AgNPs were characterized through various bioanalytical methods. The synthesis was confirmed by Bio- spectrophotometry, which showed an absorption peak at 404 nm. FTIR analysis verified the reduction and capping of Con-AgNPs, displaying peaks corresponding to various functional groups. SEM-EDAX and HR-TEM examinations revealed that the Con-AgNPs were spherical, and EDAX analysis confirmed the presence of silver atoms at 3 keV. XRD studies revealed the crystalline structure of Con-AgNPs. DLS and Zeta potential tests determined the size and stability of the synthesized Con-AgNPs, which were 65.81 nm. The Con-AgNPs demonstrated strong antibacterial activity against P. aeruginosa (14.06 ± 0.11 mm, 10 mg/mL) and effective antifungal activity against A. flavus (13.03 ± 0.05 mm, 10 mg/mL). Con-AgNPs exhibited notable biological attributes, including a cytotoxic effect of up to 38.82% and 19.15% at 200 µg/mL in an MTT assay measuring cell viability. Additionally, the nanoparticles demonstrated significant anti-inflammatory effects in both in vitro and in vivo studies, validating the biological and pharmacological potential of Con-AgNPs. |
| format | Article |
| id | doaj-art-a3caae6a9aca4fbd85c7ec2d340ca3c2 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-a3caae6a9aca4fbd85c7ec2d340ca3c22025-01-05T12:20:47ZengNature PortfolioScientific Reports2045-23222025-01-0115112910.1038/s41598-024-76475-xPharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus Coniothyrium chaingmaiense: KUMBMDBT-25Manjunatha Dadayya0Megha Gowri Thippeswamy1Nagaraju Shivaiah2Thoyajakshi Ramasamudra Siddaraju3Prakash Jayaramaiah4Sowmya Hirakannavar Veeranna5Thippeswamy Basaiah6Shridhar N Mathad7Ravikumar Hemagiri Gowda8Sachin Naik9Abdulaziz Abdullah AI Kheraif10Sajith Vellappally11Department of P.G. Studies and Research in Microbiology, Bioscience Complex, Kuvempu UniversityDepartment of P.G. Studies and Research in Biochemistry, Kuvempu UniversityDepartment of Studies and Research in Biochemistry, Tumkur UniversityDepartment of Studies and Research in Biotechnology, Tumkur UniversityDepartment of P.G. Studies and Research in Microbiology, Bioscience Complex, Kuvempu UniversityDepartment of P.G. Studies and Research in Microbiology, Bioscience Complex, Kuvempu UniversityDepartment of P.G. Studies and Research in Microbiology, Bioscience Complex, Kuvempu UniversityDepartment of Physics, KLE Institute of TechnologyDepartment of Life Sciences, Bangalore UniversityDental Health Department, College of Applied Medical Sciences, King Saud UniversityDental Health Department, College of Applied Medical Sciences, King Saud UniversityDental Health Department, College of Applied Medical Sciences, King Saud UniversityAbstract In this study, the endophytic fungus Coniothyrium chaingmaiense-KUMBMDBT-25 was isolated from the healthy stem of Euphorbia tirucalli, mass cultivated by submerged fermentation, and extracted using ethyl acetate as a solvent. The extract was subjected to GC-MS analysis. The synthesized Con-AgNPs were characterized through various bioanalytical methods. The synthesis was confirmed by Bio- spectrophotometry, which showed an absorption peak at 404 nm. FTIR analysis verified the reduction and capping of Con-AgNPs, displaying peaks corresponding to various functional groups. SEM-EDAX and HR-TEM examinations revealed that the Con-AgNPs were spherical, and EDAX analysis confirmed the presence of silver atoms at 3 keV. XRD studies revealed the crystalline structure of Con-AgNPs. DLS and Zeta potential tests determined the size and stability of the synthesized Con-AgNPs, which were 65.81 nm. The Con-AgNPs demonstrated strong antibacterial activity against P. aeruginosa (14.06 ± 0.11 mm, 10 mg/mL) and effective antifungal activity against A. flavus (13.03 ± 0.05 mm, 10 mg/mL). Con-AgNPs exhibited notable biological attributes, including a cytotoxic effect of up to 38.82% and 19.15% at 200 µg/mL in an MTT assay measuring cell viability. Additionally, the nanoparticles demonstrated significant anti-inflammatory effects in both in vitro and in vivo studies, validating the biological and pharmacological potential of Con-AgNPs.https://doi.org/10.1038/s41598-024-76475-xEndophytic fungusConiothyrium chaingmaienseGC-MSCon-AgNPsPharmacological properties |
| spellingShingle | Manjunatha Dadayya Megha Gowri Thippeswamy Nagaraju Shivaiah Thoyajakshi Ramasamudra Siddaraju Prakash Jayaramaiah Sowmya Hirakannavar Veeranna Thippeswamy Basaiah Shridhar N Mathad Ravikumar Hemagiri Gowda Sachin Naik Abdulaziz Abdullah AI Kheraif Sajith Vellappally Pharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus Coniothyrium chaingmaiense: KUMBMDBT-25 Scientific Reports Endophytic fungus Coniothyrium chaingmaiense GC-MS Con-AgNPs Pharmacological properties |
| title | Pharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus Coniothyrium chaingmaiense: KUMBMDBT-25 |
| title_full | Pharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus Coniothyrium chaingmaiense: KUMBMDBT-25 |
| title_fullStr | Pharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus Coniothyrium chaingmaiense: KUMBMDBT-25 |
| title_full_unstemmed | Pharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus Coniothyrium chaingmaiense: KUMBMDBT-25 |
| title_short | Pharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus Coniothyrium chaingmaiense: KUMBMDBT-25 |
| title_sort | pharmacological properties of biomimetic synthesized silver nanoparticles from endophytic fungus coniothyrium chaingmaiense kumbmdbt 25 |
| topic | Endophytic fungus Coniothyrium chaingmaiense GC-MS Con-AgNPs Pharmacological properties |
| url | https://doi.org/10.1038/s41598-024-76475-x |
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