The Synergistic Mechanism of <i>Chelidonium majus</i> Alkaloids on Melanoma Treatment via a Multi-Strategy Insight
Melanoma represents a formidable challenge in dermatological oncology due to its resistance to conventional treatments. The Celandine Alkali Injection Formula (CAIF) offers benefits on clinical internal medicine treatments, within which chelidonine and tetrandrine are recognized as potential quality...
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MDPI AG
2024-11-01
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| author | Peng Chen Xin-Ye Ji Jian-Ting Feng Xiao-Qin Wang Bo Zhang |
| author_facet | Peng Chen Xin-Ye Ji Jian-Ting Feng Xiao-Qin Wang Bo Zhang |
| author_sort | Peng Chen |
| collection | DOAJ |
| description | Melanoma represents a formidable challenge in dermatological oncology due to its resistance to conventional treatments. The Celandine Alkali Injection Formula (CAIF) offers benefits on clinical internal medicine treatments, within which chelidonine and tetrandrine are recognized as potential quality markers. However, their synergistic mechanisms facilitating their anti-melanoma action remain unveiled. This study embarked on an exploration of CAIF’s therapeutic potential through a multifaceted research design, integrating system pharmacological predictions with empirical molecular biological evaluations. The dual application of chelidonine and tetrandrine within CAIF exhibited a pronounced inhibitory effect on the proliferation of B16F10 cells, surpassing the effectiveness of individual compound administration. Computational predictions identified the top 50 targets, involved in key signaling pathways including cell cycle regulation, and melanogenesis. RNA sequencing further elucidated that the combinatory treatment modulated a broader spectrum of differentially expressed genes, implicating crucial biological processes including cell differentiation, and tyrosinase metabolism. The combination markedly enhanced melanogenesis and apoptotic indices, arrested cell cycle progression, and fostered cellular differentiation. Notably, chelidonine additionally curtailed the migratory capacity of B16F10 cells. Our findings underscore the therapeutic potential of chelidonine and tetrandrine, key components of CAIF, in effectively combating melanoma by targeting cell proliferation, migration, differentiation, and melanogenesis. |
| format | Article |
| id | doaj-art-a384fb56a0ab441dbaced99327d1f536 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-a384fb56a0ab441dbaced99327d1f5362024-11-26T18:15:55ZengMDPI AGMolecules1420-30492024-11-012922541210.3390/molecules29225412The Synergistic Mechanism of <i>Chelidonium majus</i> Alkaloids on Melanoma Treatment via a Multi-Strategy InsightPeng Chen0Xin-Ye Ji1Jian-Ting Feng2Xiao-Qin Wang3Bo Zhang4Key Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, Chengdu 610106, ChinaKey Laboratory of Xinjiang Phytomedicine Resources and Utilization, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832003, ChinaKey Laboratory of Xinjiang Phytomedicine Resources and Utilization, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832003, ChinaKey Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, Chengdu 610106, ChinaKey Laboratory of Medicinal and Edible Plants Resources Development of Sichuan Education Department, Sichuan Industrial Institute of Antibiotics, School of Pharmacy, Chengdu University, Chengdu 610106, ChinaMelanoma represents a formidable challenge in dermatological oncology due to its resistance to conventional treatments. The Celandine Alkali Injection Formula (CAIF) offers benefits on clinical internal medicine treatments, within which chelidonine and tetrandrine are recognized as potential quality markers. However, their synergistic mechanisms facilitating their anti-melanoma action remain unveiled. This study embarked on an exploration of CAIF’s therapeutic potential through a multifaceted research design, integrating system pharmacological predictions with empirical molecular biological evaluations. The dual application of chelidonine and tetrandrine within CAIF exhibited a pronounced inhibitory effect on the proliferation of B16F10 cells, surpassing the effectiveness of individual compound administration. Computational predictions identified the top 50 targets, involved in key signaling pathways including cell cycle regulation, and melanogenesis. RNA sequencing further elucidated that the combinatory treatment modulated a broader spectrum of differentially expressed genes, implicating crucial biological processes including cell differentiation, and tyrosinase metabolism. The combination markedly enhanced melanogenesis and apoptotic indices, arrested cell cycle progression, and fostered cellular differentiation. Notably, chelidonine additionally curtailed the migratory capacity of B16F10 cells. Our findings underscore the therapeutic potential of chelidonine and tetrandrine, key components of CAIF, in effectively combating melanoma by targeting cell proliferation, migration, differentiation, and melanogenesis.https://www.mdpi.com/1420-3049/29/22/5412melanomacombination therapymuti-targetnetwork pharmacology |
| spellingShingle | Peng Chen Xin-Ye Ji Jian-Ting Feng Xiao-Qin Wang Bo Zhang The Synergistic Mechanism of <i>Chelidonium majus</i> Alkaloids on Melanoma Treatment via a Multi-Strategy Insight Molecules melanoma combination therapy muti-target network pharmacology |
| title | The Synergistic Mechanism of <i>Chelidonium majus</i> Alkaloids on Melanoma Treatment via a Multi-Strategy Insight |
| title_full | The Synergistic Mechanism of <i>Chelidonium majus</i> Alkaloids on Melanoma Treatment via a Multi-Strategy Insight |
| title_fullStr | The Synergistic Mechanism of <i>Chelidonium majus</i> Alkaloids on Melanoma Treatment via a Multi-Strategy Insight |
| title_full_unstemmed | The Synergistic Mechanism of <i>Chelidonium majus</i> Alkaloids on Melanoma Treatment via a Multi-Strategy Insight |
| title_short | The Synergistic Mechanism of <i>Chelidonium majus</i> Alkaloids on Melanoma Treatment via a Multi-Strategy Insight |
| title_sort | synergistic mechanism of i chelidonium majus i alkaloids on melanoma treatment via a multi strategy insight |
| topic | melanoma combination therapy muti-target network pharmacology |
| url | https://www.mdpi.com/1420-3049/29/22/5412 |
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