Endo 180 participates in collagen remodeling of the periodontal ligament during orthodontic tooth movement
Abstract Background Orthodontic tooth movement (OTM) relies on the remodeling of periodontal tissues, including the periodontal ligament (PDL) and alveolar bone. Collagen remodeling plays a crucial role during this process, allowing for the necessary changes in the PDL’s structure and function. Endo...
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BMC
2024-12-01
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| Series: | BMC Oral Health |
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| Online Access: | https://doi.org/10.1186/s12903-024-05362-8 |
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| author | Liyuan Chen Danqing He Zixin Li Shengjie Cui Min Yu Zimo Zhao Yuetong Chen Jiayi Song Nan Jiang Huajie Yu Yan Liu |
| author_facet | Liyuan Chen Danqing He Zixin Li Shengjie Cui Min Yu Zimo Zhao Yuetong Chen Jiayi Song Nan Jiang Huajie Yu Yan Liu |
| author_sort | Liyuan Chen |
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| description | Abstract Background Orthodontic tooth movement (OTM) relies on the remodeling of periodontal tissues, including the periodontal ligament (PDL) and alveolar bone. Collagen remodeling plays a crucial role during this process, allowing for the necessary changes in the PDL’s structure and function. Endo180, an urokinase plasminogen activator receptor-associated protein, is a transmembrane receptor regulated collagen remodeling. This study aims to investigate whether and how Endo180 participates in collagen remodeling within the PDL during OTM. Materials and methods A mechanical force-induced OTM rat model was established using a closed coiled spring to mesially move the right maxillary first molar. The distance of OTM was examined by micro-computed tomography (micro-CT). The collagen remodeling within the PDL was assessed using atomic force microscope (AFM), Hematoxylin-Eosin (HE) staining and Masson staining. Protein expressions of Endo180, collagen I (COL I) and collagen III (COL III) were analyzed via immunofluorescence staining. Additionally, the mRNA expressions of Endo180, COL I, and COL III in force-induced PDL cells were examined by RT-qPCR in vitro. To further illustrate the role of Endo180 in regulating COL I and COL III expressions, Endo180 siRNA (siEndo) was applied to force-stimulated PDL cells. Results Force application increased OTM distance and disrupted collagen fiber organization, with a greater decrease in collagen elastic modulus on the mesial side than on the distal side of the PDL. After 7 days of force application, Endo180 and COL III expressions significantly increased in PDL tissues, while COL I expression decreased in PDL tissues. Compressive force loading in vitro upregulated the mRNA expressions of Endo180 and COL III, but downregulated COL I mRNA expression. Notably, Endo180 knockdown using siRNA suppressed force-induced COL III expression while restoring the downregulated COL I expression under compressive force stimuli. Conclusion Force-induced Endo180 expression modulates collagen remodeling in PDL during OTM by upregulating COL III and downregulating COL I. This collagen reorganization facilitates efficient tooth movement, highlighting Endo180 as a potential therapeutic target to optimize orthodontic treatment outcomes. |
| format | Article |
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| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
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| series | BMC Oral Health |
| spelling | doaj-art-a35c84e265a14f9fbe45e7acaebafb2a2025-01-05T12:48:30ZengBMCBMC Oral Health1472-68312024-12-0124111010.1186/s12903-024-05362-8Endo 180 participates in collagen remodeling of the periodontal ligament during orthodontic tooth movementLiyuan Chen0Danqing He1Zixin Li2Shengjie Cui3Min Yu4Zimo Zhao5Yuetong Chen6Jiayi Song7Nan Jiang8Huajie Yu9Yan Liu10Department of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University SchoolDepartment of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University SchoolDepartment of Stomatology, Peking University People’s HospitalDepartment of General Dentistry, Peking University School and Hospital of StomatologyDepartment of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University SchoolDepartment of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University SchoolDepartment of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University SchoolDepartment of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University SchoolCentral Laboratory, Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital StomatologyPeking University Hospital of Stomatology Fourth DivisionDepartment of Orthodontics, Central Laboratory, Hospital for Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & Research Center of Engineering and Technology for Computerized Dentistry Ministry of Health & NMPA Key Laboratory for Dental Materials & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices, Peking University SchoolAbstract Background Orthodontic tooth movement (OTM) relies on the remodeling of periodontal tissues, including the periodontal ligament (PDL) and alveolar bone. Collagen remodeling plays a crucial role during this process, allowing for the necessary changes in the PDL’s structure and function. Endo180, an urokinase plasminogen activator receptor-associated protein, is a transmembrane receptor regulated collagen remodeling. This study aims to investigate whether and how Endo180 participates in collagen remodeling within the PDL during OTM. Materials and methods A mechanical force-induced OTM rat model was established using a closed coiled spring to mesially move the right maxillary first molar. The distance of OTM was examined by micro-computed tomography (micro-CT). The collagen remodeling within the PDL was assessed using atomic force microscope (AFM), Hematoxylin-Eosin (HE) staining and Masson staining. Protein expressions of Endo180, collagen I (COL I) and collagen III (COL III) were analyzed via immunofluorescence staining. Additionally, the mRNA expressions of Endo180, COL I, and COL III in force-induced PDL cells were examined by RT-qPCR in vitro. To further illustrate the role of Endo180 in regulating COL I and COL III expressions, Endo180 siRNA (siEndo) was applied to force-stimulated PDL cells. Results Force application increased OTM distance and disrupted collagen fiber organization, with a greater decrease in collagen elastic modulus on the mesial side than on the distal side of the PDL. After 7 days of force application, Endo180 and COL III expressions significantly increased in PDL tissues, while COL I expression decreased in PDL tissues. Compressive force loading in vitro upregulated the mRNA expressions of Endo180 and COL III, but downregulated COL I mRNA expression. Notably, Endo180 knockdown using siRNA suppressed force-induced COL III expression while restoring the downregulated COL I expression under compressive force stimuli. Conclusion Force-induced Endo180 expression modulates collagen remodeling in PDL during OTM by upregulating COL III and downregulating COL I. This collagen reorganization facilitates efficient tooth movement, highlighting Endo180 as a potential therapeutic target to optimize orthodontic treatment outcomes.https://doi.org/10.1186/s12903-024-05362-8Endo180Collagen remodelingOrthodontic tooth movementMechanical forcePeriodontal ligament cell |
| spellingShingle | Liyuan Chen Danqing He Zixin Li Shengjie Cui Min Yu Zimo Zhao Yuetong Chen Jiayi Song Nan Jiang Huajie Yu Yan Liu Endo 180 participates in collagen remodeling of the periodontal ligament during orthodontic tooth movement BMC Oral Health Endo180 Collagen remodeling Orthodontic tooth movement Mechanical force Periodontal ligament cell |
| title | Endo 180 participates in collagen remodeling of the periodontal ligament during orthodontic tooth movement |
| title_full | Endo 180 participates in collagen remodeling of the periodontal ligament during orthodontic tooth movement |
| title_fullStr | Endo 180 participates in collagen remodeling of the periodontal ligament during orthodontic tooth movement |
| title_full_unstemmed | Endo 180 participates in collagen remodeling of the periodontal ligament during orthodontic tooth movement |
| title_short | Endo 180 participates in collagen remodeling of the periodontal ligament during orthodontic tooth movement |
| title_sort | endo 180 participates in collagen remodeling of the periodontal ligament during orthodontic tooth movement |
| topic | Endo180 Collagen remodeling Orthodontic tooth movement Mechanical force Periodontal ligament cell |
| url | https://doi.org/10.1186/s12903-024-05362-8 |
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