基于16S rDNA基因测序探讨咳嗽变异性哮喘与肠道菌群的关系
目的基于“肺与大肠相表里”中医理论和16S核糖体DNA(16S rDNA)基因测序技术,探讨卵清蛋白(OVA)诱导的咳嗽变异性哮喘(CVA)对小鼠肠道菌群的影响。方法20只BALB/c雌性小鼠随机分为模型组和对照组,以OVA致敏并激发制备CVA小鼠模型。记录小鼠体质量、咳嗽次数及咳嗽潜伏期,肺组织进行病理学检查,16S rDNA高通量测序分析粪便菌群。结果与对照组相比,模型组小鼠一般状态较差,躁动不安,抓耳挠腮,体质量下降(<italic>P</italic>< 0.01),咳嗽次数增多(<italic>P</italic>&...
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Main Authors: | 沈灵, 曾琳琳, 骆巧媚, 范依霖, 贺倩, 段小云 |
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Format: | Article |
Language: | zho |
Published: |
Editorial Office of Journal of Sun Yat-sen University
2022-07-01
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Series: | Zhongshan Daxue xuebao. Yixue kexue ban |
Subjects: | |
Online Access: | http://xuebaoyx.sysu.edu.cn/zh/article/doi/10.13471/j.cnki.j.sun.yat-sen.univ(med.sci).2022.0408/ |
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