Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application
Cardiovascular stent has been widely applied to treat cardiovascular disease (CVD), which is the major disease contribution to mortality in the world wide. Biodegradable magnesium (Mg) alloys are the encouraging materials in cardiovascular stents benefit from absorbability and biocompatibility. Whil...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-12-01
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| Series: | Journal of Magnesium and Alloys |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213956723001032 |
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| author | Ya-chen Hou Jing-an Li Chang Cao Chang Su Zhen Qin Ge Zhang Jia-cheng Guo Jun-nan Tang Jin-ying Zhang Shao-kang Guan |
| author_facet | Ya-chen Hou Jing-an Li Chang Cao Chang Su Zhen Qin Ge Zhang Jia-cheng Guo Jun-nan Tang Jin-ying Zhang Shao-kang Guan |
| author_sort | Ya-chen Hou |
| collection | DOAJ |
| description | Cardiovascular stent has been widely applied to treat cardiovascular disease (CVD), which is the major disease contribution to mortality in the world wide. Biodegradable magnesium (Mg) alloys are the encouraging materials in cardiovascular stents benefit from absorbability and biocompatibility. While, the ability of degradation is a double-edged sword for manufacture stent, modifying the surface to decrease the excessive degradation rate and promote the surface endothelialization could expand the prospect of the further application. In this work, the biodegradable Mg-Zn-Y-Nd alloy was modified by MgF2 and dopamine polymer film (PDA) as the corrosion resistance layer and the bonding layer respectively, and then the exosome, a natural nanoparticle contains mRNAs and proteins, was tailored to give the surface better biocompatibility. The electrochemical test and weight loss test reflected the MgF2-PDA/exosome coating increase the corrosion resistance of the Mg-Zn-Y-Nd alloy. The cytocompatibility data indicated the novel MgF2-PDA/exosome coating selectively reduced the tumor necrosis factor (TNF-α) expression and ROS release from macrophage, and promoted the α-SMA expression of smooth muscle cells. In addition, the MgF2-PDA/exosome coating also improved the adhesion, proliferation, CD31 expression and nitric oxide (NO) release of vascular endothelial cells (ECs), all of which contribute to the surface endothelialization. And the mechanism experiments showed the exosome released from the coating uptake by the ECs and assemble around the lysosome and mitochondria, and the released rate of the exosome on the coating is around 5 to 7 days, indicating excellent multi-functions of MgF2-PDA/exosome coating in cardiovascular stent. |
| format | Article |
| id | doaj-art-a2c8e21232af447584c36bdfb861b52c |
| institution | Kabale University |
| issn | 2213-9567 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Magnesium and Alloys |
| spelling | doaj-art-a2c8e21232af447584c36bdfb861b52c2025-01-10T04:37:54ZengKeAi Communications Co., Ltd.Journal of Magnesium and Alloys2213-95672024-12-01121249885004Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent applicationYa-chen Hou0Jing-an Li1Chang Cao2Chang Su3Zhen Qin4Ge Zhang5Jia-cheng Guo6Jun-nan Tang7Jin-ying Zhang8Shao-kang Guan9Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Henan Province Key Laboratory of Cardiac Injury and Repair, Zhengzhou, Henan 450052, China; Henan Province Clinical Research Center for Cardiovascular Diseases, Zhengzhou, Henan 450018, China; School of Material Science and Engineering & Henan Key Laboratory of Advanced Magnesium Alloy & Key Laboratory of materials processing and mold technology (Ministry of Education), Zhengzhou University, 100 Science Road, Zhengzhou 450001, ChinaSchool of Material Science and Engineering & Henan Key Laboratory of Advanced Magnesium Alloy & Key Laboratory of materials processing and mold technology (Ministry of Education), Zhengzhou University, 100 Science Road, Zhengzhou 450001, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Henan Province Key Laboratory of Cardiac Injury and Repair, Zhengzhou, Henan 450052, China; Henan Province Clinical Research Center for Cardiovascular Diseases, Zhengzhou, Henan 450018, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Henan Province Key Laboratory of Cardiac Injury and Repair, Zhengzhou, Henan 450052, China; Henan Province Clinical Research Center for Cardiovascular Diseases, Zhengzhou, Henan 450018, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Henan Province Key Laboratory of Cardiac Injury and Repair, Zhengzhou, Henan 450052, China; Henan Province Clinical Research Center for Cardiovascular Diseases, Zhengzhou, Henan 450018, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Henan Province Key Laboratory of Cardiac Injury and Repair, Zhengzhou, Henan 450052, China; Henan Province Clinical Research Center for Cardiovascular Diseases, Zhengzhou, Henan 450018, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Henan Province Key Laboratory of Cardiac Injury and Repair, Zhengzhou, Henan 450052, China; Henan Province Clinical Research Center for Cardiovascular Diseases, Zhengzhou, Henan 450018, ChinaDepartment of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Henan Province Key Laboratory of Cardiac Injury and Repair, Zhengzhou, Henan 450052, China; Henan Province Clinical Research Center for Cardiovascular Diseases, Zhengzhou, Henan 450018, China; Corresponding authors at: Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China; Henan Province Key Laboratory of Cardiac Injury and Repair, Zhengzhou, Henan 450052, China; Henan Province Clinical Research Center for Cardiovascular Diseases, Zhengzhou, Henan 450018, China; Corresponding authors at: Department of Cardiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China.School of Material Science and Engineering & Henan Key Laboratory of Advanced Magnesium Alloy & Key Laboratory of materials processing and mold technology (Ministry of Education), Zhengzhou University, 100 Science Road, Zhengzhou 450001, China; Corresponding author.Cardiovascular stent has been widely applied to treat cardiovascular disease (CVD), which is the major disease contribution to mortality in the world wide. Biodegradable magnesium (Mg) alloys are the encouraging materials in cardiovascular stents benefit from absorbability and biocompatibility. While, the ability of degradation is a double-edged sword for manufacture stent, modifying the surface to decrease the excessive degradation rate and promote the surface endothelialization could expand the prospect of the further application. In this work, the biodegradable Mg-Zn-Y-Nd alloy was modified by MgF2 and dopamine polymer film (PDA) as the corrosion resistance layer and the bonding layer respectively, and then the exosome, a natural nanoparticle contains mRNAs and proteins, was tailored to give the surface better biocompatibility. The electrochemical test and weight loss test reflected the MgF2-PDA/exosome coating increase the corrosion resistance of the Mg-Zn-Y-Nd alloy. The cytocompatibility data indicated the novel MgF2-PDA/exosome coating selectively reduced the tumor necrosis factor (TNF-α) expression and ROS release from macrophage, and promoted the α-SMA expression of smooth muscle cells. In addition, the MgF2-PDA/exosome coating also improved the adhesion, proliferation, CD31 expression and nitric oxide (NO) release of vascular endothelial cells (ECs), all of which contribute to the surface endothelialization. And the mechanism experiments showed the exosome released from the coating uptake by the ECs and assemble around the lysosome and mitochondria, and the released rate of the exosome on the coating is around 5 to 7 days, indicating excellent multi-functions of MgF2-PDA/exosome coating in cardiovascular stent.http://www.sciencedirect.com/science/article/pii/S2213956723001032Mg-Zn-Y-Nd alloyEndothelializationSurface modificationMgF2-PDA/exosome coating |
| spellingShingle | Ya-chen Hou Jing-an Li Chang Cao Chang Su Zhen Qin Ge Zhang Jia-cheng Guo Jun-nan Tang Jin-ying Zhang Shao-kang Guan Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application Journal of Magnesium and Alloys Mg-Zn-Y-Nd alloy Endothelialization Surface modification MgF2-PDA/exosome coating |
| title | Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application |
| title_full | Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application |
| title_fullStr | Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application |
| title_full_unstemmed | Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application |
| title_short | Biodegradable Mg alloy modified with bioactive exosomes for cardiovascular stent application |
| title_sort | biodegradable mg alloy modified with bioactive exosomes for cardiovascular stent application |
| topic | Mg-Zn-Y-Nd alloy Endothelialization Surface modification MgF2-PDA/exosome coating |
| url | http://www.sciencedirect.com/science/article/pii/S2213956723001032 |
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