Recovery of filter paperase from mouldy rice husk in solid state fermentation by Aspergillus protuberus
Recovery of enzymes such as FPase (filter paperase) or exoglucanase from fermented substrate is a sustainable approach in enzyme production; however, there is a scarcity of optimization studies in this field. The present study was aimed to standardize number of parameters (selection of solvent, solv...
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| Format: | Article |
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Elsevier
2024-01-01
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| Series: | Current Research in Microbial Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517424000361 |
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| author | P.Suresh Yadav B.V.Siva Prasad M.Subhosh Chandra M. Srinivasulu Naga Raju Maddela Ram Prasad |
| author_facet | P.Suresh Yadav B.V.Siva Prasad M.Subhosh Chandra M. Srinivasulu Naga Raju Maddela Ram Prasad |
| author_sort | P.Suresh Yadav |
| collection | DOAJ |
| description | Recovery of enzymes such as FPase (filter paperase) or exoglucanase from fermented substrate is a sustainable approach in enzyme production; however, there is a scarcity of optimization studies in this field. The present study was aimed to standardize number of parameters (selection of solvent, solvent volume, soaking time, leaching conditions and number of washes) to extract maximum amount of FPase from fermented rice husk by Aspergillus protuberus. Novel Aspergillus protuberus was first report from our lab on cellulases production in solid state fermentation (SSF). Among the tested solvents, citrate phosphate buffer (0.02 M, pH 5.0) proved best solvent for maximum recovery of FPase. Consequent experimental parameters were further optimized with citrate phosphate buffer. Two washes with citrate phosphate buffer each by shaking (60 min) in a ratio of 1 g of rice husk: 5 ml of citrate phosphate buffer together attained higher recovery efficiency (88 %) of FPase from the fermented rice husk. |
| format | Article |
| id | doaj-art-a2c78160e35c4735bf3fccf6ea3f7458 |
| institution | Kabale University |
| issn | 2666-5174 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj-art-a2c78160e35c4735bf3fccf6ea3f74582024-12-04T05:14:01ZengElsevierCurrent Research in Microbial Sciences2666-51742024-01-017100254Recovery of filter paperase from mouldy rice husk in solid state fermentation by Aspergillus protuberusP.Suresh Yadav0B.V.Siva Prasad1M.Subhosh Chandra2M. Srinivasulu3Naga Raju Maddela4Ram Prasad5Department of Microbiology, Yogi Vemana University, Kadapa, Andhra Pradesh, IndiaDepartment of Microbiology, Yogi Vemana University, Kadapa, Andhra Pradesh, IndiaDepartment of Microbiology, Yogi Vemana University, Kadapa, Andhra Pradesh, IndiaDepartment of Biotechnology, Yogi Vemana University, Kadapa, Andhra Pradesh, IndiaDepartamento de Ciencias Biológicas, Facultad de Ciencias de la Salud, Universidad Técnica de Manabí, Portoviejo, Manabí, EcuadorDepartment of Botany, Mahatma Gandhi Central University, Motihari 845401, Bihar, IndiaRecovery of enzymes such as FPase (filter paperase) or exoglucanase from fermented substrate is a sustainable approach in enzyme production; however, there is a scarcity of optimization studies in this field. The present study was aimed to standardize number of parameters (selection of solvent, solvent volume, soaking time, leaching conditions and number of washes) to extract maximum amount of FPase from fermented rice husk by Aspergillus protuberus. Novel Aspergillus protuberus was first report from our lab on cellulases production in solid state fermentation (SSF). Among the tested solvents, citrate phosphate buffer (0.02 M, pH 5.0) proved best solvent for maximum recovery of FPase. Consequent experimental parameters were further optimized with citrate phosphate buffer. Two washes with citrate phosphate buffer each by shaking (60 min) in a ratio of 1 g of rice husk: 5 ml of citrate phosphate buffer together attained higher recovery efficiency (88 %) of FPase from the fermented rice husk.http://www.sciencedirect.com/science/article/pii/S2666517424000361Aspergillus protuberusFPaseExtractionSolid state fermentationRice husk |
| spellingShingle | P.Suresh Yadav B.V.Siva Prasad M.Subhosh Chandra M. Srinivasulu Naga Raju Maddela Ram Prasad Recovery of filter paperase from mouldy rice husk in solid state fermentation by Aspergillus protuberus Current Research in Microbial Sciences Aspergillus protuberus FPase Extraction Solid state fermentation Rice husk |
| title | Recovery of filter paperase from mouldy rice husk in solid state fermentation by Aspergillus protuberus |
| title_full | Recovery of filter paperase from mouldy rice husk in solid state fermentation by Aspergillus protuberus |
| title_fullStr | Recovery of filter paperase from mouldy rice husk in solid state fermentation by Aspergillus protuberus |
| title_full_unstemmed | Recovery of filter paperase from mouldy rice husk in solid state fermentation by Aspergillus protuberus |
| title_short | Recovery of filter paperase from mouldy rice husk in solid state fermentation by Aspergillus protuberus |
| title_sort | recovery of filter paperase from mouldy rice husk in solid state fermentation by aspergillus protuberus |
| topic | Aspergillus protuberus FPase Extraction Solid state fermentation Rice husk |
| url | http://www.sciencedirect.com/science/article/pii/S2666517424000361 |
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