Genetic Association Analysis of Paratuberculosis Forms in Holstein-Friesian Cattle
A genetic susceptibility to Mycobacterium avium subsp. paratuberculosis (MAP) infections in ruminants has been longtime suspected to exist. Recently, natural infections in cattle have been reclassified into latent and patent forms based on histopathological findings and their associations with immun...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2014/321327 |
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| author | Patricia Vázquez Otsanda Ruiz-Larrañaga Joseba M. Garrido Mikel Iriondo Carmen Manzano Mikel Agirre Andone Estonba Ramón A. Juste |
| author_facet | Patricia Vázquez Otsanda Ruiz-Larrañaga Joseba M. Garrido Mikel Iriondo Carmen Manzano Mikel Agirre Andone Estonba Ramón A. Juste |
| author_sort | Patricia Vázquez |
| collection | DOAJ |
| description | A genetic susceptibility to Mycobacterium avium subsp. paratuberculosis (MAP) infections in ruminants has been longtime suspected to exist. Recently, natural infections in cattle have been reclassified into latent and patent forms based on histopathological findings and their associations with immunological and microbiological variables. This study aims to explore whether these newly defined phenotypes are associated with twenty-four single-nucleotide polymorphisms (SNPs) in six bovine candidate genes: nucleotide-binding oligomerization domain 2 (NOD2), solute carrier family 11 member A1 (SLC11A1), nuclear body protein SP110 (SP110), toll-like receptors (TLRs) 2 and 4, and CD209 (also known as DC-SIGN, dendritic cell-specific ICAM3-grabbing nonintegrin). SNPs were genotyped for 772 Holstein-Friesian animals (52.6% apparently free; 38.1% latent; 9.3% patent) by TaqMan OpenArray technology. Genotypic-phenotypic associations were assessed by logistic regression analysis adjusted for age at slaughter, under five models (codominant, dominant, recessive, overdominant, and log-additive), and corrected for multiple testing. The rs208222804 C allele (CD209 gene) was found to be associated with latent paratuberculosis (log-additive model: P<0.0034 after permutation procedure; OR = 0.64, 95% CI = 0.48–0.86). No significant association was detected between any SNP and the patent phenotype. Consequently, CD209 gene may play a key role in the pathogenesis of bovine paratuberculosis. |
| format | Article |
| id | doaj-art-a2aed2f3bb8d45b8ba7e10d9d3f9a7d9 |
| institution | OA Journals |
| issn | 2090-8113 2042-0048 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-a2aed2f3bb8d45b8ba7e10d9d3f9a7d92025-08-20T02:20:37ZengWileyVeterinary Medicine International2090-81132042-00482014-01-01201410.1155/2014/321327321327Genetic Association Analysis of Paratuberculosis Forms in Holstein-Friesian CattlePatricia Vázquez0Otsanda Ruiz-Larrañaga1Joseba M. Garrido2Mikel Iriondo3Carmen Manzano4Mikel Agirre5Andone Estonba6Ramón A. Juste7Department of Animal Health, NEIKER-Tecnalia, Berreaga 1, 48160 Derio, Bizkaia, SpainDepartment of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, 48940 Leioa, Bizkaia, SpainDepartment of Animal Health, NEIKER-Tecnalia, Berreaga 1, 48160 Derio, Bizkaia, SpainDepartment of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, 48940 Leioa, Bizkaia, SpainDepartment of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, 48940 Leioa, Bizkaia, SpainDepartment of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, 48940 Leioa, Bizkaia, SpainDepartment of Genetics, Physical Anthropology and Animal Physiology, Faculty of Science and Technology, University of the Basque Country (UPV/EHU), Barrio Sarriena S/N, 48940 Leioa, Bizkaia, SpainDepartment of Animal Health, NEIKER-Tecnalia, Berreaga 1, 48160 Derio, Bizkaia, SpainA genetic susceptibility to Mycobacterium avium subsp. paratuberculosis (MAP) infections in ruminants has been longtime suspected to exist. Recently, natural infections in cattle have been reclassified into latent and patent forms based on histopathological findings and their associations with immunological and microbiological variables. This study aims to explore whether these newly defined phenotypes are associated with twenty-four single-nucleotide polymorphisms (SNPs) in six bovine candidate genes: nucleotide-binding oligomerization domain 2 (NOD2), solute carrier family 11 member A1 (SLC11A1), nuclear body protein SP110 (SP110), toll-like receptors (TLRs) 2 and 4, and CD209 (also known as DC-SIGN, dendritic cell-specific ICAM3-grabbing nonintegrin). SNPs were genotyped for 772 Holstein-Friesian animals (52.6% apparently free; 38.1% latent; 9.3% patent) by TaqMan OpenArray technology. Genotypic-phenotypic associations were assessed by logistic regression analysis adjusted for age at slaughter, under five models (codominant, dominant, recessive, overdominant, and log-additive), and corrected for multiple testing. The rs208222804 C allele (CD209 gene) was found to be associated with latent paratuberculosis (log-additive model: P<0.0034 after permutation procedure; OR = 0.64, 95% CI = 0.48–0.86). No significant association was detected between any SNP and the patent phenotype. Consequently, CD209 gene may play a key role in the pathogenesis of bovine paratuberculosis.http://dx.doi.org/10.1155/2014/321327 |
| spellingShingle | Patricia Vázquez Otsanda Ruiz-Larrañaga Joseba M. Garrido Mikel Iriondo Carmen Manzano Mikel Agirre Andone Estonba Ramón A. Juste Genetic Association Analysis of Paratuberculosis Forms in Holstein-Friesian Cattle Veterinary Medicine International |
| title | Genetic Association Analysis of Paratuberculosis Forms in Holstein-Friesian Cattle |
| title_full | Genetic Association Analysis of Paratuberculosis Forms in Holstein-Friesian Cattle |
| title_fullStr | Genetic Association Analysis of Paratuberculosis Forms in Holstein-Friesian Cattle |
| title_full_unstemmed | Genetic Association Analysis of Paratuberculosis Forms in Holstein-Friesian Cattle |
| title_short | Genetic Association Analysis of Paratuberculosis Forms in Holstein-Friesian Cattle |
| title_sort | genetic association analysis of paratuberculosis forms in holstein friesian cattle |
| url | http://dx.doi.org/10.1155/2014/321327 |
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