Ginkgo biloba L. extract and flunixin meglumine attenuate sepsis–associated liver injury, oxidative stress, inflammation and apoptosis in rats

Lipopolysaccharide (LPS), known as a stimulant of inflammation, causes acute liver injury by inducing the production of inflammatory mediators and oxidative stress. The purpose of this study is to determine whether of a nonsteroidal anti–inflammatory drug (NSAID) Flunixin meglumine (FM) and herbal...

Full description

Saved in:
Bibliographic Details
Main Authors: Tuba Parlak Ak, Burcu Gul, Mine Yaman, Ismail Seven, Gurdal Dagoglu, Huseyin Fatih Gul
Format: Article
Language:English
Published: Universidad del Zulia 2024-12-01
Series:Revista Científica
Subjects:
Online Access:https://mail.produccioncientificaluz.org/index.php/cientifica/article/view/43156
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1846102295450746880
author Tuba Parlak Ak
Burcu Gul
Mine Yaman
Ismail Seven
Gurdal Dagoglu
Huseyin Fatih Gul
author_facet Tuba Parlak Ak
Burcu Gul
Mine Yaman
Ismail Seven
Gurdal Dagoglu
Huseyin Fatih Gul
author_sort Tuba Parlak Ak
collection DOAJ
description Lipopolysaccharide (LPS), known as a stimulant of inflammation, causes acute liver injury by inducing the production of inflammatory mediators and oxidative stress. The purpose of this study is to determine whether of a nonsteroidal anti–inflammatory drug (NSAID) Flunixin meglumine (FM) and herbal an medicine Ginkgo biloba L. extract (GBE) show antioxidative, anti–inflammatory or antiapoptotic effects in liver tissue in LPS–induced hepatotoxicity. Animals were separated to 6 groups as control, sepsis (1 mg·kg-1, 7th day single dose, intraperitoneal (ip)), sepsis + FM (1 mg·kg-1, 7th day single dose, ip + 2.2 mg·kg-1 day, ip), sepsis + GBE (1 mg·kg-1, 7th day single dose, ip + 50 mg·kg-1 day, gavage), FM and GBE and the study continued for 7 days. Liver tissues taken from rats sacrificed were analyzed biochemically, histologically and immunohistochemically. Accordingly, LPS caused liver function markers alteration, inflammation, oxidative stress, and apoptosis, as well as histopathological changes in liver tissue. However, it was observed that LPS–induced changes were regulated by FM and GBE application. FM and GBE was demonstrated to have antioxidant, antiinflammatory and anti–apoptotic properties in LPS–induced hepatotoxicity.
format Article
id doaj-art-a2a76ed8b496493ca96fef8ca566220a
institution Kabale University
issn 0798-2259
2521-9715
language English
publishDate 2024-12-01
publisher Universidad del Zulia
record_format Article
series Revista Científica
spelling doaj-art-a2a76ed8b496493ca96fef8ca566220a2024-12-27T15:36:08ZengUniversidad del ZuliaRevista Científica0798-22592521-97152024-12-0134310.52973/rcfcv-e34501Ginkgo biloba L. extract and flunixin meglumine attenuate sepsis–associated liver injury, oxidative stress, inflammation and apoptosis in ratsTuba Parlak Ak0Burcu Gul1Mine Yaman2Ismail Seven3Gurdal Dagoglu4Huseyin Fatih Gul5University of Munzur, Faculty of Health Sciences, Department of Nutrition and Dietetics.Tunceli, TürkiyeUniversity of Firat, Faculty of Health Sciences, Department of Nursing. Elazig, TürkiyeUniversity of Firat, Faculty of Veterinary Medicine, Department of Histology and Embryology. Elazig, TürkiyeUniversity of Firat, Vocational School of Sivrice, Department of Plant and Animal Production. Elazig, TürkiyeUniversity of Firat, Faculty of Veterinary Medicine, Department of Pharmacology and Toxicology. Elazig, TürkiyeUniversity of Kafkas, Faculty of Medicine, Department of Biochemistry. Kars, Türkiye Lipopolysaccharide (LPS), known as a stimulant of inflammation, causes acute liver injury by inducing the production of inflammatory mediators and oxidative stress. The purpose of this study is to determine whether of a nonsteroidal anti–inflammatory drug (NSAID) Flunixin meglumine (FM) and herbal an medicine Ginkgo biloba L. extract (GBE) show antioxidative, anti–inflammatory or antiapoptotic effects in liver tissue in LPS–induced hepatotoxicity. Animals were separated to 6 groups as control, sepsis (1 mg·kg-1, 7th day single dose, intraperitoneal (ip)), sepsis + FM (1 mg·kg-1, 7th day single dose, ip + 2.2 mg·kg-1 day, ip), sepsis + GBE (1 mg·kg-1, 7th day single dose, ip + 50 mg·kg-1 day, gavage), FM and GBE and the study continued for 7 days. Liver tissues taken from rats sacrificed were analyzed biochemically, histologically and immunohistochemically. Accordingly, LPS caused liver function markers alteration, inflammation, oxidative stress, and apoptosis, as well as histopathological changes in liver tissue. However, it was observed that LPS–induced changes were regulated by FM and GBE application. FM and GBE was demonstrated to have antioxidant, antiinflammatory and anti–apoptotic properties in LPS–induced hepatotoxicity. https://mail.produccioncientificaluz.org/index.php/cientifica/article/view/43156Flunixin meglumineGinkgo biloba L. extractliver damagesepsis
spellingShingle Tuba Parlak Ak
Burcu Gul
Mine Yaman
Ismail Seven
Gurdal Dagoglu
Huseyin Fatih Gul
Ginkgo biloba L. extract and flunixin meglumine attenuate sepsis–associated liver injury, oxidative stress, inflammation and apoptosis in rats
Revista Científica
Flunixin meglumine
Ginkgo biloba L. extract
liver damage
sepsis
title Ginkgo biloba L. extract and flunixin meglumine attenuate sepsis–associated liver injury, oxidative stress, inflammation and apoptosis in rats
title_full Ginkgo biloba L. extract and flunixin meglumine attenuate sepsis–associated liver injury, oxidative stress, inflammation and apoptosis in rats
title_fullStr Ginkgo biloba L. extract and flunixin meglumine attenuate sepsis–associated liver injury, oxidative stress, inflammation and apoptosis in rats
title_full_unstemmed Ginkgo biloba L. extract and flunixin meglumine attenuate sepsis–associated liver injury, oxidative stress, inflammation and apoptosis in rats
title_short Ginkgo biloba L. extract and flunixin meglumine attenuate sepsis–associated liver injury, oxidative stress, inflammation and apoptosis in rats
title_sort ginkgo biloba l extract and flunixin meglumine attenuate sepsis associated liver injury oxidative stress inflammation and apoptosis in rats
topic Flunixin meglumine
Ginkgo biloba L. extract
liver damage
sepsis
url https://mail.produccioncientificaluz.org/index.php/cientifica/article/view/43156
work_keys_str_mv AT tubaparlakak ginkgobilobalextractandflunixinmeglumineattenuatesepsisassociatedliverinjuryoxidativestressinflammationandapoptosisinrats
AT burcugul ginkgobilobalextractandflunixinmeglumineattenuatesepsisassociatedliverinjuryoxidativestressinflammationandapoptosisinrats
AT mineyaman ginkgobilobalextractandflunixinmeglumineattenuatesepsisassociatedliverinjuryoxidativestressinflammationandapoptosisinrats
AT ismailseven ginkgobilobalextractandflunixinmeglumineattenuatesepsisassociatedliverinjuryoxidativestressinflammationandapoptosisinrats
AT gurdaldagoglu ginkgobilobalextractandflunixinmeglumineattenuatesepsisassociatedliverinjuryoxidativestressinflammationandapoptosisinrats
AT huseyinfatihgul ginkgobilobalextractandflunixinmeglumineattenuatesepsisassociatedliverinjuryoxidativestressinflammationandapoptosisinrats