Screening and characterization of integration sites based on CRISPR-Cpf1 in Pichia pastoris

Screening and characterization of integration sites based on CRISPR-Cpf1 in Pichia pastoris

Pichia pastoris, a methylotrophic yeast, can utilize methanol as a carbon source and energy source to synthesize high-value chemicals, and is an ideal host for biomanufacturing. Constructing the P. pastoris cell factory is somewhat impeded due to the absence of genetic tools for manipulating multi-g...

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Bibliographic Details
Main Authors: Shupeng Ruan, Yuxin Yang, Xinying Zhang, Guanjuan Luo, Ying Lin, Shuli Liang
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2024-12-01
Series:Synthetic and Systems Biotechnology
Subjects:
P. pastoris
CRISPR-Cpf1
Integration sites
Metabolic engineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2405805X24000917
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