The effect of microRNA-145 on proliferation and apoptosis of cutaneous squamous cell carcinoma cells
Abstract Objective This study aimed to explore the effects of microRNA−145 (miR−145) on the proliferation, cell cycle distribution, and apoptosis of cutaneous squamous cell carcinoma (CSCC) A431 cells. Methods A431 cells were transfected with miR−145 mimics and negative control microRNA using Lipofe...
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| Format: | Article |
| Language: | English |
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Springer
2025-07-01
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| Series: | Discover Oncology |
| Online Access: | https://doi.org/10.1007/s12672-025-03032-x |
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| _version_ | 1849334324649787392 |
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| author | Gengjian Zhang Songlian Lei Kaiyue Zhang |
| author_facet | Gengjian Zhang Songlian Lei Kaiyue Zhang |
| author_sort | Gengjian Zhang |
| collection | DOAJ |
| description | Abstract Objective This study aimed to explore the effects of microRNA−145 (miR−145) on the proliferation, cell cycle distribution, and apoptosis of cutaneous squamous cell carcinoma (CSCC) A431 cells. Methods A431 cells were transfected with miR−145 mimics and negative control microRNA using Lipofectamine 2000. The experiments were divided into three groups: the miR−145 mimics transfection group, the negative control microRNA transfection group, and the blank control group (without any treatment). Results The expression levels of miR−145 were significantly higher in the miR−145 mimics group than in the negative control microRNA group and the blank control group (p < 0.01). The cell proliferation level of the miR−145 mimics group was significantly lower at 24 h, 48 h, and 72 h post-transfection compared to the negative control microRNA group and the blank control group (p < 0.01). The proportion of cells in the G0/G1 phase was significantly higher in the miR−145 mimics group, while the percentage of cells in the S phase was significantly lower (p < 0.01). Additionally, the apoptosis rate in the miR−145 mimics group was significantly higher than that in the negative control microRNA group and the blank control group (p < 0.01). The relative expression levels of Caspase−9 and Caspase−3 were also significantly higher in the miR−145 mimics group (p < 0.01). Conclusion Upregulation of miR−145 expression by miR−145 mimics transfection in CSCC A431 cells significantly inhibited cell proliferation, blocked cell cycle progression at the G0/G1 phase, and promoted apoptosis by increasing the expression of apoptotic proteins Caspase−9 and Caspase−3. These findings suggest that miR−145 may serve as a potential anti-tumor factor in CSCC, highlighting its potential as a therapeutic target for future research. |
| format | Article |
| id | doaj-art-a268e97fb706434dba24a9a99c4409b5 |
| institution | Kabale University |
| issn | 2730-6011 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Springer |
| record_format | Article |
| series | Discover Oncology |
| spelling | doaj-art-a268e97fb706434dba24a9a99c4409b52025-08-20T03:45:35ZengSpringerDiscover Oncology2730-60112025-07-0116111110.1007/s12672-025-03032-xThe effect of microRNA-145 on proliferation and apoptosis of cutaneous squamous cell carcinoma cellsGengjian Zhang0Songlian Lei1Kaiyue Zhang2Department of Dermatology, Affiliated Hospital of Zunyi Medical UniversityDepartment of Dermatology, Affiliated Hospital of Zunyi Medical UniversityDepartment of Dermatology, Affiliated Hospital of Zunyi Medical UniversityAbstract Objective This study aimed to explore the effects of microRNA−145 (miR−145) on the proliferation, cell cycle distribution, and apoptosis of cutaneous squamous cell carcinoma (CSCC) A431 cells. Methods A431 cells were transfected with miR−145 mimics and negative control microRNA using Lipofectamine 2000. The experiments were divided into three groups: the miR−145 mimics transfection group, the negative control microRNA transfection group, and the blank control group (without any treatment). Results The expression levels of miR−145 were significantly higher in the miR−145 mimics group than in the negative control microRNA group and the blank control group (p < 0.01). The cell proliferation level of the miR−145 mimics group was significantly lower at 24 h, 48 h, and 72 h post-transfection compared to the negative control microRNA group and the blank control group (p < 0.01). The proportion of cells in the G0/G1 phase was significantly higher in the miR−145 mimics group, while the percentage of cells in the S phase was significantly lower (p < 0.01). Additionally, the apoptosis rate in the miR−145 mimics group was significantly higher than that in the negative control microRNA group and the blank control group (p < 0.01). The relative expression levels of Caspase−9 and Caspase−3 were also significantly higher in the miR−145 mimics group (p < 0.01). Conclusion Upregulation of miR−145 expression by miR−145 mimics transfection in CSCC A431 cells significantly inhibited cell proliferation, blocked cell cycle progression at the G0/G1 phase, and promoted apoptosis by increasing the expression of apoptotic proteins Caspase−9 and Caspase−3. These findings suggest that miR−145 may serve as a potential anti-tumor factor in CSCC, highlighting its potential as a therapeutic target for future research.https://doi.org/10.1007/s12672-025-03032-x |
| spellingShingle | Gengjian Zhang Songlian Lei Kaiyue Zhang The effect of microRNA-145 on proliferation and apoptosis of cutaneous squamous cell carcinoma cells Discover Oncology |
| title | The effect of microRNA-145 on proliferation and apoptosis of cutaneous squamous cell carcinoma cells |
| title_full | The effect of microRNA-145 on proliferation and apoptosis of cutaneous squamous cell carcinoma cells |
| title_fullStr | The effect of microRNA-145 on proliferation and apoptosis of cutaneous squamous cell carcinoma cells |
| title_full_unstemmed | The effect of microRNA-145 on proliferation and apoptosis of cutaneous squamous cell carcinoma cells |
| title_short | The effect of microRNA-145 on proliferation and apoptosis of cutaneous squamous cell carcinoma cells |
| title_sort | effect of microrna 145 on proliferation and apoptosis of cutaneous squamous cell carcinoma cells |
| url | https://doi.org/10.1007/s12672-025-03032-x |
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