Danhong injection alleviates OGD-induced blood-brain barrier injury via VEGFR2/PI3K/AKT pathway based on network pharmacology and experimental evidence
Objective: Previous studies have shown that Danhong injection (DHI) has a protective effect on the blood-brain barrier (BBB) function after ischemic stroke. However, the role of DHI in protecting the integrity of the BBB after ischemic stroke through endothelial cells is still unclear. The purpose o...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2024-03-01
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| Series: | Journal of Holistic Integrative Pharmacy |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2707368824000165 |
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| author | Yutong Zhang Meixia Xie Jiayin Liang Li Li Shumei Wang Minghua Xian |
| author_facet | Yutong Zhang Meixia Xie Jiayin Liang Li Li Shumei Wang Minghua Xian |
| author_sort | Yutong Zhang |
| collection | DOAJ |
| description | Objective: Previous studies have shown that Danhong injection (DHI) has a protective effect on the blood-brain barrier (BBB) function after ischemic stroke. However, the role of DHI in protecting the integrity of the BBB after ischemic stroke through endothelial cells is still unclear. The purpose of this study is to explore the role and mechanism of DHI in protecting BBB by interfering with endothelial cells. Methods: We used network pharmacology technology to determine the potential pathways and mechanisms of DHI in treating ischemic stroke through endothelial cells. On account of the network pharmacology results, we assessed the effects of DHI in oxygen-glucose deprivation (OGD) model of mouse brain-derived endothelial (bEnd.3) cells via MTT, and validated the molecular mechanisms of DHI improving BBB injury through Western blot. Results: Network pharmacology analysis suggested that DHI may regulate the PI3K/AKT signaling pathway of endothelial cells in the treatment of ischemic stroke. Cellular experiments showed that DHI stimulates endothelial cell migration and reduces OGD-induced BBB damage via VEGFR2/PI3K/AKT pathway. Conclusion: Network pharmacology analysis and cellular experiments have shown that DHI alleviated the BBB damage by activating the VEGFR2/PI3K/AKT signaling pathway on endothelial cells. |
| format | Article |
| id | doaj-art-a25e3b233b114cbb872ebdbfb6c85e5f |
| institution | Kabale University |
| issn | 2707-3688 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Journal of Holistic Integrative Pharmacy |
| spelling | doaj-art-a25e3b233b114cbb872ebdbfb6c85e5f2024-11-12T05:21:47ZengKeAi Communications Co., Ltd.Journal of Holistic Integrative Pharmacy2707-36882024-03-01511018Danhong injection alleviates OGD-induced blood-brain barrier injury via VEGFR2/PI3K/AKT pathway based on network pharmacology and experimental evidenceYutong Zhang0Meixia Xie1Jiayin Liang2Li Li3Shumei Wang4Minghua Xian5Guangdong Pharmaceutical University, Guangzhou, 510006, ChinaGuangdong Pharmaceutical University, Guangzhou, 510006, China; Key Laboratory of Digital Quality Evaluation of Traditional Chinese Medicine, National Administration of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China; Traditional Chinese Medicine Quality Engineering and Technology Research Center of Guangdong Universities, Guangdong Pharmaceutical University, Guangzhou, 510006, ChinaGuangdong Pharmaceutical University, Guangzhou, 510006, China; Key Laboratory of Digital Quality Evaluation of Traditional Chinese Medicine, National Administration of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China; Traditional Chinese Medicine Quality Engineering and Technology Research Center of Guangdong Universities, Guangdong Pharmaceutical University, Guangzhou, 510006, ChinaCelularity.Inc, 170 Park Ave, Florham Park, New Jersey, 07932, USAGuangdong Pharmaceutical University, Guangzhou, 510006, China; Key Laboratory of Digital Quality Evaluation of Traditional Chinese Medicine, National Administration of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China; Traditional Chinese Medicine Quality Engineering and Technology Research Center of Guangdong Universities, Guangdong Pharmaceutical University, Guangzhou, 510006, China; Traditional Chinese Medicine Resource Germplasm Bank Management Center, Yunfu, 527322, China; Corresponding author. Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, 280 Waihuan Rd E, Guangzhou, Guangdong, 510006, China.Guangdong Pharmaceutical University, Guangzhou, 510006, China; Key Laboratory of Digital Quality Evaluation of Traditional Chinese Medicine, National Administration of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, 510006, China; Traditional Chinese Medicine Quality Engineering and Technology Research Center of Guangdong Universities, Guangdong Pharmaceutical University, Guangzhou, 510006, China; Corresponding author. Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, 280 Waihuan Rd E, Guangzhou, Guangdong, 510006, ChinaObjective: Previous studies have shown that Danhong injection (DHI) has a protective effect on the blood-brain barrier (BBB) function after ischemic stroke. However, the role of DHI in protecting the integrity of the BBB after ischemic stroke through endothelial cells is still unclear. The purpose of this study is to explore the role and mechanism of DHI in protecting BBB by interfering with endothelial cells. Methods: We used network pharmacology technology to determine the potential pathways and mechanisms of DHI in treating ischemic stroke through endothelial cells. On account of the network pharmacology results, we assessed the effects of DHI in oxygen-glucose deprivation (OGD) model of mouse brain-derived endothelial (bEnd.3) cells via MTT, and validated the molecular mechanisms of DHI improving BBB injury through Western blot. Results: Network pharmacology analysis suggested that DHI may regulate the PI3K/AKT signaling pathway of endothelial cells in the treatment of ischemic stroke. Cellular experiments showed that DHI stimulates endothelial cell migration and reduces OGD-induced BBB damage via VEGFR2/PI3K/AKT pathway. Conclusion: Network pharmacology analysis and cellular experiments have shown that DHI alleviated the BBB damage by activating the VEGFR2/PI3K/AKT signaling pathway on endothelial cells.http://www.sciencedirect.com/science/article/pii/S2707368824000165Danhong injectionCerebral ischemic strokeNetwork pharmacologyVEGFR2/PI3K/AKT pathwayBlood-brain-barrier |
| spellingShingle | Yutong Zhang Meixia Xie Jiayin Liang Li Li Shumei Wang Minghua Xian Danhong injection alleviates OGD-induced blood-brain barrier injury via VEGFR2/PI3K/AKT pathway based on network pharmacology and experimental evidence Journal of Holistic Integrative Pharmacy Danhong injection Cerebral ischemic stroke Network pharmacology VEGFR2/PI3K/AKT pathway Blood-brain-barrier |
| title | Danhong injection alleviates OGD-induced blood-brain barrier injury via VEGFR2/PI3K/AKT pathway based on network pharmacology and experimental evidence |
| title_full | Danhong injection alleviates OGD-induced blood-brain barrier injury via VEGFR2/PI3K/AKT pathway based on network pharmacology and experimental evidence |
| title_fullStr | Danhong injection alleviates OGD-induced blood-brain barrier injury via VEGFR2/PI3K/AKT pathway based on network pharmacology and experimental evidence |
| title_full_unstemmed | Danhong injection alleviates OGD-induced blood-brain barrier injury via VEGFR2/PI3K/AKT pathway based on network pharmacology and experimental evidence |
| title_short | Danhong injection alleviates OGD-induced blood-brain barrier injury via VEGFR2/PI3K/AKT pathway based on network pharmacology and experimental evidence |
| title_sort | danhong injection alleviates ogd induced blood brain barrier injury via vegfr2 pi3k akt pathway based on network pharmacology and experimental evidence |
| topic | Danhong injection Cerebral ischemic stroke Network pharmacology VEGFR2/PI3K/AKT pathway Blood-brain-barrier |
| url | http://www.sciencedirect.com/science/article/pii/S2707368824000165 |
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