Phytochemical screening, antioxidant, and antimicrobial analysis of Portulaca oleracea seeds with in-silico molecular docking insights
Background: Portulaca oleracea (PO), an annual succulent herb with global distribution, has been used medicinally since ancient times, earning the title “global panacea.” Aim: This study aimed to perform phytochemical screening, antioxidant and antimicrobial analysis of PO seeds, including GCMS anal...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687157X25000605 |
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| author | Khursheed Ahmad Sheikh Reyaz Hassan Mir Mohammad Ovais Dar Adil Farooq Wali Insha Qadir Sheeba Nazir Mohammed Iqbal Zargar Sirajunisa Talath Sathvik B. Sridhar Javedh Shareef Mubashir Hussain Masoodi |
| author_facet | Khursheed Ahmad Sheikh Reyaz Hassan Mir Mohammad Ovais Dar Adil Farooq Wali Insha Qadir Sheeba Nazir Mohammed Iqbal Zargar Sirajunisa Talath Sathvik B. Sridhar Javedh Shareef Mubashir Hussain Masoodi |
| author_sort | Khursheed Ahmad Sheikh |
| collection | DOAJ |
| description | Background: Portulaca oleracea (PO), an annual succulent herb with global distribution, has been used medicinally since ancient times, earning the title “global panacea.” Aim: This study aimed to perform phytochemical screening, antioxidant and antimicrobial analysis of PO seeds, including GCMS analysis of methanolic extract and molecular docking for antimicrobial mechanisms. Materials and Methods: PO seeds underwent phytochemical screening and methanolic extract analysis via DPPH, NO radical scavenging, and reducing power assays. Antimicrobial activity was tested against bacteria and fungi, with GCMS identifying compounds. Molecular docking was conducted via Autodock vina, against targets beta-tubulin (5FNV) and ABC transporter (6J9W). Results: Methanolic extract showed strong antioxidant activity (IC50: 125.2 µg/ml for DPPH, 402.89 µg/ml for NO) and concentration-dependent reducing power. It was highly effective against Saccharomyces cerevisiae and Hypochrea viridescens. GCMS identified colchicine, n-decanoic acid, and triepoxydecane, with colchicine showing high binding affinity to protein targets. Conclusion: The methanolic extract’s potent antioxidant and antimicrobial effects, supported by colchicine’s binding affinity, validate PO’s medicinal potential, suggesting further therapeutic exploration. |
| format | Article |
| id | doaj-art-a251118ff76f4c458e3717879295fe7f |
| institution | Kabale University |
| issn | 1687-157X |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj-art-a251118ff76f4c458e3717879295fe7f2025-08-24T05:11:44ZengElsevierJournal of Genetic Engineering and Biotechnology1687-157X2025-09-0123310051610.1016/j.jgeb.2025.100516Phytochemical screening, antioxidant, and antimicrobial analysis of Portulaca oleracea seeds with in-silico molecular docking insightsKhursheed Ahmad Sheikh0Reyaz Hassan Mir1Mohammad Ovais Dar2Adil Farooq Wali3Insha Qadir4Sheeba Nazir5Mohammed Iqbal Zargar6Sirajunisa Talath7Sathvik B. Sridhar8Javedh Shareef9Mubashir Hussain Masoodi10Pharmaceutical Chemistry Division, Department of Pharmaceutical Sciences, School of Applied Sciences and Technology, University of Kashmir, Hazratbal, Srinagar 190006 Kashmir, India; Pharmaceutical Chemistry, School of Pharmaceutical Education and Research (SPER), Jamia Hamdard, Hamdard Nagar, New Delhi, IndiaPharmaceutical Chemistry Division, Department of Pharmaceutical Sciences, School of Applied Sciences and Technology, University of Kashmir, Hazratbal, Srinagar 190006 Kashmir, India; Corresponding authors.Department of Pharmaceutical Chemistry, M. M. College of Pharmacy, Maharishi Markandeshwar (Deemed to be University), Mullana, Ambala, Haryana 133207, IndiaDepartment of Pharmaceutical Chemistry, RAK College of Pharmacy, RAK Medical and Health Science University, Ras Al Khaimah, United Arab EmiratesPharmaceutical Chemistry Division, Department of Pharmaceutical Sciences, School of Applied Sciences and Technology, University of Kashmir, Hazratbal, Srinagar 190006 Kashmir, IndiaPharmaceutical Chemistry Division, Department of Pharmaceutical Sciences, School of Applied Sciences and Technology, University of Kashmir, Hazratbal, Srinagar 190006 Kashmir, IndiaPharmaceutical Biotechnology Division Department of Pharmaceutical Sciences, School of Applied Sciences and Technology, University of Kashmir, Srinagar 190006, IndiaDepartment of Pharmaceutical Chemistry, RAK College of Pharmacy, RAK Medical and Health Science University, Ras Al Khaimah, United Arab EmiratesDepartment of Clinical Pharmacy & Pharmacology, RAK College of Pharmacy, RAK Medical & Health Sciences University, Ras Al Khaimah, United Arab EmiratesDepartment of Clinical Pharmacy & Pharmacology, RAK College of Pharmacy, RAK Medical & Health Sciences University, Ras Al Khaimah, United Arab EmiratesPharmaceutical Chemistry Division, Department of Pharmaceutical Sciences, School of Applied Sciences and Technology, University of Kashmir, Hazratbal, Srinagar 190006 Kashmir, India; Corresponding authors.Background: Portulaca oleracea (PO), an annual succulent herb with global distribution, has been used medicinally since ancient times, earning the title “global panacea.” Aim: This study aimed to perform phytochemical screening, antioxidant and antimicrobial analysis of PO seeds, including GCMS analysis of methanolic extract and molecular docking for antimicrobial mechanisms. Materials and Methods: PO seeds underwent phytochemical screening and methanolic extract analysis via DPPH, NO radical scavenging, and reducing power assays. Antimicrobial activity was tested against bacteria and fungi, with GCMS identifying compounds. Molecular docking was conducted via Autodock vina, against targets beta-tubulin (5FNV) and ABC transporter (6J9W). Results: Methanolic extract showed strong antioxidant activity (IC50: 125.2 µg/ml for DPPH, 402.89 µg/ml for NO) and concentration-dependent reducing power. It was highly effective against Saccharomyces cerevisiae and Hypochrea viridescens. GCMS identified colchicine, n-decanoic acid, and triepoxydecane, with colchicine showing high binding affinity to protein targets. Conclusion: The methanolic extract’s potent antioxidant and antimicrobial effects, supported by colchicine’s binding affinity, validate PO’s medicinal potential, suggesting further therapeutic exploration.http://www.sciencedirect.com/science/article/pii/S1687157X25000605Portulaca oleraceaPurslaneNatural compoundsAntioxidantAntibacterialAntifungal antimicrobial |
| spellingShingle | Khursheed Ahmad Sheikh Reyaz Hassan Mir Mohammad Ovais Dar Adil Farooq Wali Insha Qadir Sheeba Nazir Mohammed Iqbal Zargar Sirajunisa Talath Sathvik B. Sridhar Javedh Shareef Mubashir Hussain Masoodi Phytochemical screening, antioxidant, and antimicrobial analysis of Portulaca oleracea seeds with in-silico molecular docking insights Journal of Genetic Engineering and Biotechnology Portulaca oleracea Purslane Natural compounds Antioxidant Antibacterial Antifungal antimicrobial |
| title | Phytochemical screening, antioxidant, and antimicrobial analysis of Portulaca oleracea seeds with in-silico molecular docking insights |
| title_full | Phytochemical screening, antioxidant, and antimicrobial analysis of Portulaca oleracea seeds with in-silico molecular docking insights |
| title_fullStr | Phytochemical screening, antioxidant, and antimicrobial analysis of Portulaca oleracea seeds with in-silico molecular docking insights |
| title_full_unstemmed | Phytochemical screening, antioxidant, and antimicrobial analysis of Portulaca oleracea seeds with in-silico molecular docking insights |
| title_short | Phytochemical screening, antioxidant, and antimicrobial analysis of Portulaca oleracea seeds with in-silico molecular docking insights |
| title_sort | phytochemical screening antioxidant and antimicrobial analysis of portulaca oleracea seeds with in silico molecular docking insights |
| topic | Portulaca oleracea Purslane Natural compounds Antioxidant Antibacterial Antifungal antimicrobial |
| url | http://www.sciencedirect.com/science/article/pii/S1687157X25000605 |
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