A fast in situ hybridization chain reaction method in Drosophila embryos and ovaries
The in situ hybridization chain reaction (isHCR) is a powerful method for visualizing mRNA in many species. We present a rapid isHCR method for Drosophila embryos and ovaries. Ethylene carbonate was added to the hybridization buffer to facilitate the hybridization reaction, and a modified short hair...
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| Main Authors: | Kyohei Mikami, Yasuhiro Kozono, Masaki Masukawa, Satoru Kobayashi |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
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| Series: | Fly |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19336934.2024.2428499 |
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