The extremely low-frequency electromagnetic field (50 Hz) can establish a new “set-point” for the activity of the locus coeruleus–noradrenergic (LC-NA) system in rat

Exposure of organisms to extremely low-frequency electromagnetic field (ELF-EMF; 50 Hz) has been increasing in recent decades, which is connected with dynamic technological development. ELF-EMF is considered a stress factor and its effects on organisms are still being investigated. We aimed to deter...

Full description

Saved in:
Bibliographic Details
Main Authors: Angelika Klimek, Hanna Kletkiewicz, Agnieszka Siejka, Joanna Wyszkowska, Justyna Maliszewska, Maciej Klimiuk, Milena Jankowska, Justyna Rogalska
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Brain Research Bulletin
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0361923024002454
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1846151055090384896
author Angelika Klimek
Hanna Kletkiewicz
Agnieszka Siejka
Joanna Wyszkowska
Justyna Maliszewska
Maciej Klimiuk
Milena Jankowska
Justyna Rogalska
author_facet Angelika Klimek
Hanna Kletkiewicz
Agnieszka Siejka
Joanna Wyszkowska
Justyna Maliszewska
Maciej Klimiuk
Milena Jankowska
Justyna Rogalska
author_sort Angelika Klimek
collection DOAJ
description Exposure of organisms to extremely low-frequency electromagnetic field (ELF-EMF; 50 Hz) has been increasing in recent decades, which is connected with dynamic technological development. ELF-EMF is considered a stress factor and its effects on organisms are still being investigated. We aimed to determine its impact on the locus coeruleus–noradrenergic (LC-NA) system enabling adaptation to stressful conditions. For this purpose, we exposed rats to 50 Hz ELF-EMF of 1 and 7 mT, 1 h/day for 7 days. The procedure was repeated three times to examine the organism's adaptive capabilities. Subsequently, the concentration of adrenaline, noradrenaline and its metabolite MHPG as well as the expression of the β2-adrenergic receptor was assessed. After the end of each exposure, part of the animals were subjected to a behavioural test to assess the influence of repeated ELF-EMF exposure on stress response to subsequent stress factors. Our research proved that mechanisms underlying the effects of ELF-EMF on stress response include the LC-NA system. ELF-EMF of 1 mT induced adaptive changes in the NA-LC system. However, exposure to 7 mT caused increased activity of the stress system which resulted in sensitization to subsequent, heterotypic (different from the one previously acting) stress factor. As ELF-EMF of 7 mT caused a profound decrease in β2-AR level would strongly inhibit the potential for neuroplastic processes in the hippocampus. Moreover, rats exposed to ELF-EMF of 7 mT showed moderately increased anxiety-related behaviour. Disturbances in NA-LC transmission may underlie the development of some neurodegenerative and psychiatric diseases which indicates the possible involvement of ELF-EMF in the pathogenesis of these disorders.
format Article
id doaj-art-a217b87faa964a79a0a4c3e13e19e50c
institution Kabale University
issn 1873-2747
language English
publishDate 2024-12-01
publisher Elsevier
record_format Article
series Brain Research Bulletin
spelling doaj-art-a217b87faa964a79a0a4c3e13e19e50c2024-11-28T04:34:03ZengElsevierBrain Research Bulletin1873-27472024-12-01219111111The extremely low-frequency electromagnetic field (50 Hz) can establish a new “set-point” for the activity of the locus coeruleus–noradrenergic (LC-NA) system in ratAngelika Klimek0Hanna Kletkiewicz1Agnieszka Siejka2Joanna Wyszkowska3Justyna Maliszewska4Maciej Klimiuk5Milena Jankowska6Justyna Rogalska7Department of Exercise Physiology and Functional Anatomy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Bydgoszcz 85-077, Poland; Correspondence to: Department of Exercise Physiology and Functional Anatomy, Ludwik Rydygier Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Torun, Świętojańska 20, Bydgoszcz 85-077, PolandDepartment of Animal Physiology and Neurobiology, Faculty of Biological and Veterinary Sciences Nicolaus Copernicus University in Torun, 87-100, PolandDepartment of Animal Physiology and Neurobiology, Faculty of Biological and Veterinary Sciences Nicolaus Copernicus University in Torun, 87-100, PolandDepartment of Animal Physiology and Neurobiology, Faculty of Biological and Veterinary Sciences Nicolaus Copernicus University in Torun, 87-100, PolandDepartment of Animal Physiology and Neurobiology, Faculty of Biological and Veterinary Sciences Nicolaus Copernicus University in Torun, 87-100, PolandDepartment of Animal Physiology and Neurobiology, Faculty of Biological and Veterinary Sciences Nicolaus Copernicus University in Torun, 87-100, PolandDepartment of Animal Physiology and Neurobiology, Faculty of Biological and Veterinary Sciences Nicolaus Copernicus University in Torun, 87-100, PolandDepartment of Animal Physiology and Neurobiology, Faculty of Biological and Veterinary Sciences Nicolaus Copernicus University in Torun, 87-100, Poland; Correspondence to: Department of Animal Physiology and Neurobiology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Lwowska 1, Torun 87-100, PolandExposure of organisms to extremely low-frequency electromagnetic field (ELF-EMF; 50 Hz) has been increasing in recent decades, which is connected with dynamic technological development. ELF-EMF is considered a stress factor and its effects on organisms are still being investigated. We aimed to determine its impact on the locus coeruleus–noradrenergic (LC-NA) system enabling adaptation to stressful conditions. For this purpose, we exposed rats to 50 Hz ELF-EMF of 1 and 7 mT, 1 h/day for 7 days. The procedure was repeated three times to examine the organism's adaptive capabilities. Subsequently, the concentration of adrenaline, noradrenaline and its metabolite MHPG as well as the expression of the β2-adrenergic receptor was assessed. After the end of each exposure, part of the animals were subjected to a behavioural test to assess the influence of repeated ELF-EMF exposure on stress response to subsequent stress factors. Our research proved that mechanisms underlying the effects of ELF-EMF on stress response include the LC-NA system. ELF-EMF of 1 mT induced adaptive changes in the NA-LC system. However, exposure to 7 mT caused increased activity of the stress system which resulted in sensitization to subsequent, heterotypic (different from the one previously acting) stress factor. As ELF-EMF of 7 mT caused a profound decrease in β2-AR level would strongly inhibit the potential for neuroplastic processes in the hippocampus. Moreover, rats exposed to ELF-EMF of 7 mT showed moderately increased anxiety-related behaviour. Disturbances in NA-LC transmission may underlie the development of some neurodegenerative and psychiatric diseases which indicates the possible involvement of ELF-EMF in the pathogenesis of these disorders.http://www.sciencedirect.com/science/article/pii/S0361923024002454Extremely low-frequency electromagnetic fieldStress responseLocus coeruleusNoradrenalineβ2- adrenergic receptorHippocampus
spellingShingle Angelika Klimek
Hanna Kletkiewicz
Agnieszka Siejka
Joanna Wyszkowska
Justyna Maliszewska
Maciej Klimiuk
Milena Jankowska
Justyna Rogalska
The extremely low-frequency electromagnetic field (50 Hz) can establish a new “set-point” for the activity of the locus coeruleus–noradrenergic (LC-NA) system in rat
Brain Research Bulletin
Extremely low-frequency electromagnetic field
Stress response
Locus coeruleus
Noradrenaline
β2- adrenergic receptor
Hippocampus
title The extremely low-frequency electromagnetic field (50 Hz) can establish a new “set-point” for the activity of the locus coeruleus–noradrenergic (LC-NA) system in rat
title_full The extremely low-frequency electromagnetic field (50 Hz) can establish a new “set-point” for the activity of the locus coeruleus–noradrenergic (LC-NA) system in rat
title_fullStr The extremely low-frequency electromagnetic field (50 Hz) can establish a new “set-point” for the activity of the locus coeruleus–noradrenergic (LC-NA) system in rat
title_full_unstemmed The extremely low-frequency electromagnetic field (50 Hz) can establish a new “set-point” for the activity of the locus coeruleus–noradrenergic (LC-NA) system in rat
title_short The extremely low-frequency electromagnetic field (50 Hz) can establish a new “set-point” for the activity of the locus coeruleus–noradrenergic (LC-NA) system in rat
title_sort extremely low frequency electromagnetic field 50 hz can establish a new set point for the activity of the locus coeruleus noradrenergic lc na system in rat
topic Extremely low-frequency electromagnetic field
Stress response
Locus coeruleus
Noradrenaline
β2- adrenergic receptor
Hippocampus
url http://www.sciencedirect.com/science/article/pii/S0361923024002454
work_keys_str_mv AT angelikaklimek theextremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT hannakletkiewicz theextremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT agnieszkasiejka theextremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT joannawyszkowska theextremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT justynamaliszewska theextremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT maciejklimiuk theextremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT milenajankowska theextremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT justynarogalska theextremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT angelikaklimek extremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT hannakletkiewicz extremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT agnieszkasiejka extremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT joannawyszkowska extremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT justynamaliszewska extremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT maciejklimiuk extremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT milenajankowska extremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat
AT justynarogalska extremelylowfrequencyelectromagneticfield50hzcanestablishanewsetpointfortheactivityofthelocuscoeruleusnoradrenergiclcnasysteminrat