Glycemic Control in Type 2 Diabetes Mellitus Patients Could Affect NLRP3 Inflammasome Level
Background: Inflammasome complex such as nucleotide oligomerization domain-like receptor family protein 3 (NLRP3) acts as a trigger initiating inflammatory responses and could lead to endothelial dysfunction in diabetic patients and glycemic control could affect the mitochondrial stress through NLRP...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Medical Journal of Babylon |
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| Online Access: | https://doi.org/10.4103/MJBL.MJBL_799_23 |
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| author | Refif Sabih Al-Shawk Zainab Fadhel Ashoor Wasan Abdul-elah Bakir Intisar Manfi Ahmed |
| author_facet | Refif Sabih Al-Shawk Zainab Fadhel Ashoor Wasan Abdul-elah Bakir Intisar Manfi Ahmed |
| author_sort | Refif Sabih Al-Shawk |
| collection | DOAJ |
| description | Background: Inflammasome complex such as nucleotide oligomerization domain-like receptor family protein 3 (NLRP3) acts as a trigger initiating inflammatory responses and could lead to endothelial dysfunction in diabetic patients and glycemic control could affect the mitochondrial stress through NLRP3 inflammasome level. Objectives: This study was conducted to ensure that type 2 diabetes mellitus (T2DM) glycemic control could affect mitochondrial stress through NLRP3 inflammasome level leading to an aberrant immune response. Materials and Methods: A case–control study was conducted on 90 Iraqi subjects, 60 of them were T2DM who were subgrouped into 30 patients with good glycemic control (HbA1c ≤ 7%) and the second group with 30 patients with poor (bad) glycemic control (HbA1c > 7%). Also, 30 healthy control subjects were enrolled in this study. HbA1c, fasting blood glucose (FBG), and serum levels of NLRP3 and interferon (IFN)-γ were quantitatively determined by means of a sandwich enzyme-linked immunosorbent assay (ELISA) test. Results: Results of this study showed a significant increase in serum levels of NLRP3 and IFN-γ in the poor glycemic control group of patients as compared to control subjects (P ≤ 0.05), while this difference was not significant when comparing the good glycemic control group of patients with control subjects. There is a significant positive correlation of serum NLRP3 with only IFN-γ (P ≤ 0.05) in both good and poor glycemic control and healthy controls. Conclusion: An increased level of NLRP3 was observed in poor glycemic control T2DM and correlated with IFN-γ, suggesting hyperglycemia’s effect on this inflammasome pathway that could be associated with aberrant cytokine induction, a key inducer of diabetic complications. |
| format | Article |
| id | doaj-art-a20e3bc2bfa04be4b6e86ff03f98e409 |
| institution | Kabale University |
| issn | 1812-156X 2312-6760 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Medical Journal of Babylon |
| spelling | doaj-art-a20e3bc2bfa04be4b6e86ff03f98e4092025-01-17T10:54:56ZengWolters Kluwer Medknow PublicationsMedical Journal of Babylon1812-156X2312-67602024-12-0121497097410.4103/MJBL.MJBL_799_23Glycemic Control in Type 2 Diabetes Mellitus Patients Could Affect NLRP3 Inflammasome LevelRefif Sabih Al-ShawkZainab Fadhel AshoorWasan Abdul-elah BakirIntisar Manfi AhmedBackground: Inflammasome complex such as nucleotide oligomerization domain-like receptor family protein 3 (NLRP3) acts as a trigger initiating inflammatory responses and could lead to endothelial dysfunction in diabetic patients and glycemic control could affect the mitochondrial stress through NLRP3 inflammasome level. Objectives: This study was conducted to ensure that type 2 diabetes mellitus (T2DM) glycemic control could affect mitochondrial stress through NLRP3 inflammasome level leading to an aberrant immune response. Materials and Methods: A case–control study was conducted on 90 Iraqi subjects, 60 of them were T2DM who were subgrouped into 30 patients with good glycemic control (HbA1c ≤ 7%) and the second group with 30 patients with poor (bad) glycemic control (HbA1c > 7%). Also, 30 healthy control subjects were enrolled in this study. HbA1c, fasting blood glucose (FBG), and serum levels of NLRP3 and interferon (IFN)-γ were quantitatively determined by means of a sandwich enzyme-linked immunosorbent assay (ELISA) test. Results: Results of this study showed a significant increase in serum levels of NLRP3 and IFN-γ in the poor glycemic control group of patients as compared to control subjects (P ≤ 0.05), while this difference was not significant when comparing the good glycemic control group of patients with control subjects. There is a significant positive correlation of serum NLRP3 with only IFN-γ (P ≤ 0.05) in both good and poor glycemic control and healthy controls. Conclusion: An increased level of NLRP3 was observed in poor glycemic control T2DM and correlated with IFN-γ, suggesting hyperglycemia’s effect on this inflammasome pathway that could be associated with aberrant cytokine induction, a key inducer of diabetic complications.https://doi.org/10.4103/MJBL.MJBL_799_23glycemic controlifn-γinflammasomenlrp3type 2 diabetes mellitus |
| spellingShingle | Refif Sabih Al-Shawk Zainab Fadhel Ashoor Wasan Abdul-elah Bakir Intisar Manfi Ahmed Glycemic Control in Type 2 Diabetes Mellitus Patients Could Affect NLRP3 Inflammasome Level Medical Journal of Babylon glycemic control ifn-γ inflammasome nlrp3 type 2 diabetes mellitus |
| title | Glycemic Control in Type 2 Diabetes Mellitus Patients Could Affect NLRP3 Inflammasome Level |
| title_full | Glycemic Control in Type 2 Diabetes Mellitus Patients Could Affect NLRP3 Inflammasome Level |
| title_fullStr | Glycemic Control in Type 2 Diabetes Mellitus Patients Could Affect NLRP3 Inflammasome Level |
| title_full_unstemmed | Glycemic Control in Type 2 Diabetes Mellitus Patients Could Affect NLRP3 Inflammasome Level |
| title_short | Glycemic Control in Type 2 Diabetes Mellitus Patients Could Affect NLRP3 Inflammasome Level |
| title_sort | glycemic control in type 2 diabetes mellitus patients could affect nlrp3 inflammasome level |
| topic | glycemic control ifn-γ inflammasome nlrp3 type 2 diabetes mellitus |
| url | https://doi.org/10.4103/MJBL.MJBL_799_23 |
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