Dual Probe with Fluorescent and Magnetic Properties for Imaging Solid Tumor Xenografts
A dual probe with fluorescent and magnetic reporter groups was constructed by linkage of the near-infrared (NIR) fluorescent transferrin conjugate (Tf NIR ) on the surface of contrast agent-encapsulated cationic liposome (Lip-CA). This probe was used for magnetic resonance imaging (MRI) and optical...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2007-03-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2007.00006 |
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| _version_ | 1841563161968246784 |
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| author | Liang Shan Songping Wang Rajagopalan Sridhar Zaver M. Bhujwalla Paul C. Wang |
| author_facet | Liang Shan Songping Wang Rajagopalan Sridhar Zaver M. Bhujwalla Paul C. Wang |
| author_sort | Liang Shan |
| collection | DOAJ |
| description | A dual probe with fluorescent and magnetic reporter groups was constructed by linkage of the near-infrared (NIR) fluorescent transferrin conjugate (Tf NIR ) on the surface of contrast agent-encapsulated cationic liposome (Lip-CA). This probe was used for magnetic resonance imaging (MRI) and optical imaging of MDA-MB-231-luc breast cancer cells grown as a monolayer in vitro and as solid tumor xenografts in nude mice. Confocal microscopy, optical imaging, and MRI showed a dramatic increase of in vitro cellular uptake of the fluorescent and magnetic reporter groups from the probe compared with the uptake of contrast agent or Lip-CA alone. Pretreatment with transferrin (Tf) blocked uptake of the probe reporters, indicating the importance and specificity of the Tf moiety for targeting. Intravenous administration of the dual probe to nude mice significantly enhanced the tumor contrast in MRI, and preferential accumulation of the fluorescent signal was clearly seen in NIR-based optical images. More interestingly, the contrast enhancement in MRI showed a heterogeneous pattern within tumors, which reflected the tumor's morphologic heterogeneity. These results indicate that the newly developed dual probe enhances the tumor image contrast and is superior to contrast agent alone for identifying the tumor pathologic features on the basis of MRI but also is suitable for NIR-based optical imaging. |
| format | Article |
| id | doaj-art-a1dd712af086490ab3f6437f92fcff19 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2007-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-a1dd712af086490ab3f6437f92fcff192025-01-03T00:10:40ZengSAGE PublishingMolecular Imaging1536-01212007-03-01610.2310/7290.2007.0000610.2310_7290.2007.00006Dual Probe with Fluorescent and Magnetic Properties for Imaging Solid Tumor XenograftsLiang ShanSongping WangRajagopalan SridharZaver M. BhujwallaPaul C. WangA dual probe with fluorescent and magnetic reporter groups was constructed by linkage of the near-infrared (NIR) fluorescent transferrin conjugate (Tf NIR ) on the surface of contrast agent-encapsulated cationic liposome (Lip-CA). This probe was used for magnetic resonance imaging (MRI) and optical imaging of MDA-MB-231-luc breast cancer cells grown as a monolayer in vitro and as solid tumor xenografts in nude mice. Confocal microscopy, optical imaging, and MRI showed a dramatic increase of in vitro cellular uptake of the fluorescent and magnetic reporter groups from the probe compared with the uptake of contrast agent or Lip-CA alone. Pretreatment with transferrin (Tf) blocked uptake of the probe reporters, indicating the importance and specificity of the Tf moiety for targeting. Intravenous administration of the dual probe to nude mice significantly enhanced the tumor contrast in MRI, and preferential accumulation of the fluorescent signal was clearly seen in NIR-based optical images. More interestingly, the contrast enhancement in MRI showed a heterogeneous pattern within tumors, which reflected the tumor's morphologic heterogeneity. These results indicate that the newly developed dual probe enhances the tumor image contrast and is superior to contrast agent alone for identifying the tumor pathologic features on the basis of MRI but also is suitable for NIR-based optical imaging.https://doi.org/10.2310/7290.2007.00006 |
| spellingShingle | Liang Shan Songping Wang Rajagopalan Sridhar Zaver M. Bhujwalla Paul C. Wang Dual Probe with Fluorescent and Magnetic Properties for Imaging Solid Tumor Xenografts Molecular Imaging |
| title | Dual Probe with Fluorescent and Magnetic Properties for Imaging Solid Tumor Xenografts |
| title_full | Dual Probe with Fluorescent and Magnetic Properties for Imaging Solid Tumor Xenografts |
| title_fullStr | Dual Probe with Fluorescent and Magnetic Properties for Imaging Solid Tumor Xenografts |
| title_full_unstemmed | Dual Probe with Fluorescent and Magnetic Properties for Imaging Solid Tumor Xenografts |
| title_short | Dual Probe with Fluorescent and Magnetic Properties for Imaging Solid Tumor Xenografts |
| title_sort | dual probe with fluorescent and magnetic properties for imaging solid tumor xenografts |
| url | https://doi.org/10.2310/7290.2007.00006 |
| work_keys_str_mv | AT liangshan dualprobewithfluorescentandmagneticpropertiesforimagingsolidtumorxenografts AT songpingwang dualprobewithfluorescentandmagneticpropertiesforimagingsolidtumorxenografts AT rajagopalansridhar dualprobewithfluorescentandmagneticpropertiesforimagingsolidtumorxenografts AT zavermbhujwalla dualprobewithfluorescentandmagneticpropertiesforimagingsolidtumorxenografts AT paulcwang dualprobewithfluorescentandmagneticpropertiesforimagingsolidtumorxenografts |