Application of PNR Detection in the Diagnosis and Drug-efficacy Evaluation of Diabetic Kidney Disease in Rats
Objective This study aims to monitor the mRNA ratio of podocin to nephrin (PNR) in glomerular podocytes of early diabetic kidney disease (DKD) rat models. The feasibility of using PNR as an early diagnostic indicator for DKD was evaluated by comparing it with the urinary albumin-to-creatinine ratio...
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Editorial Office of Laboratory Animal and Comparative Medicine
2024-10-01
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| Series: | Shiyan dongwu yu bijiao yixue |
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| Online Access: | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2024.044 |
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| author | ZHANG Naiqun YUAN Piaopiao CAO Linrong YING Na YANG Taotao |
| author_facet | ZHANG Naiqun YUAN Piaopiao CAO Linrong YING Na YANG Taotao |
| author_sort | ZHANG Naiqun |
| collection | DOAJ |
| description | Objective This study aims to monitor the mRNA ratio of podocin to nephrin (PNR) in glomerular podocytes of early diabetic kidney disease (DKD) rat models. The feasibility of using PNR as an early diagnostic indicator for DKD was evaluated by comparing it with the urinary albumin-to-creatinine ratio (U-ACR). Additionally, the early intervention effects of valsartan and fosinopril sodium on DKD were compared. Methods The DKD rat model was established by caudal intravenous injection of streptozotocin (STZ) at a dosage of 60 mg/kg. The early changes in PNR and U-ACR were monitored and compared, followed by timely intervention with valsartan and fosinopril sodium. Hematoxylin and eosin staining (HE) was used to observe glomerular structure, while transmission electron microscopy examined the ultrastructure of glomerular podocytes.ResultsPNR reached the critical value(≥1) on day 9 after modeling, earlier than U-ACR, which reached the critical value(≥30 mg/g) on day 15. Intervention with valsartan and fosinopril sodium on day 9 after modeling significantly reduced U-ACR (P < 0.05), with low-dose valsartan showing better results than high-dose (P>0.05), while high-dose fosinopril sodium outperformed low-dose (P>0.05). Both low doses of valsartan and fosinopril sodium significantly reduced PNR (P<0.05), with no significant effect observed for high doses. The interventions with valsartan and fosinopril sodium improved and maintained glomerular structure and podocyte arrangement.ConclusionPNR changes earlier than U-ACR, indicating its potential as an early diagnostic marker for DKD in rats. Early intervention with valsartan and fosinopril sodium demonstrates a therapeutic effect on DKD in rats. |
| format | Article |
| id | doaj-art-a1cb0d5a39014f288609c694f5ca4d19 |
| institution | Kabale University |
| issn | 1674-5817 |
| language | zho |
| publishDate | 2024-10-01 |
| publisher | Editorial Office of Laboratory Animal and Comparative Medicine |
| record_format | Article |
| series | Shiyan dongwu yu bijiao yixue |
| spelling | doaj-art-a1cb0d5a39014f288609c694f5ca4d192025-01-06T05:07:29ZzhoEditorial Office of Laboratory Animal and Comparative MedicineShiyan dongwu yu bijiao yixue1674-58172024-10-0144554354910.12300/j.issn.1674-5817.2024.0441674-5817（2024）05-0543-07Application of PNR Detection in the Diagnosis and Drug-efficacy Evaluation of Diabetic Kidney Disease in RatsZHANG Naiqun0YUAN Piaopiao1CAO Linrong2YING Na3YANG Taotao4The No.4 People's Hospital of Yuyao, Yuyao 315470, ChinaThe No.4 People's Hospital of Yuyao, Yuyao 315470, ChinaThe No.4 People's Hospital of Yuyao, Yuyao 315470, ChinaThe No.4 People's Hospital of Yuyao, Yuyao 315470, ChinaNingbo Medical Center Lihuili Hospital, Ningbo 315000, ChinaObjective This study aims to monitor the mRNA ratio of podocin to nephrin (PNR) in glomerular podocytes of early diabetic kidney disease (DKD) rat models. The feasibility of using PNR as an early diagnostic indicator for DKD was evaluated by comparing it with the urinary albumin-to-creatinine ratio (U-ACR). Additionally, the early intervention effects of valsartan and fosinopril sodium on DKD were compared. Methods The DKD rat model was established by caudal intravenous injection of streptozotocin (STZ) at a dosage of 60 mg/kg. The early changes in PNR and U-ACR were monitored and compared, followed by timely intervention with valsartan and fosinopril sodium. Hematoxylin and eosin staining (HE) was used to observe glomerular structure, while transmission electron microscopy examined the ultrastructure of glomerular podocytes.ResultsPNR reached the critical value(≥1) on day 9 after modeling, earlier than U-ACR, which reached the critical value(≥30 mg/g) on day 15. Intervention with valsartan and fosinopril sodium on day 9 after modeling significantly reduced U-ACR (P < 0.05), with low-dose valsartan showing better results than high-dose (P>0.05), while high-dose fosinopril sodium outperformed low-dose (P>0.05). Both low doses of valsartan and fosinopril sodium significantly reduced PNR (P<0.05), with no significant effect observed for high doses. The interventions with valsartan and fosinopril sodium improved and maintained glomerular structure and podocyte arrangement.ConclusionPNR changes earlier than U-ACR, indicating its potential as an early diagnostic marker for DKD in rats. Early intervention with valsartan and fosinopril sodium demonstrates a therapeutic effect on DKD in rats.https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2024.044pnrurinary albumin-to-creatinine ratio (u-acr)diabetic kidney diseaseproteinuria preventionrats |
| spellingShingle | ZHANG Naiqun YUAN Piaopiao CAO Linrong YING Na YANG Taotao Application of PNR Detection in the Diagnosis and Drug-efficacy Evaluation of Diabetic Kidney Disease in Rats Shiyan dongwu yu bijiao yixue pnr urinary albumin-to-creatinine ratio (u-acr) diabetic kidney disease proteinuria prevention rats |
| title | Application of PNR Detection in the Diagnosis and Drug-efficacy Evaluation of Diabetic Kidney Disease in Rats |
| title_full | Application of PNR Detection in the Diagnosis and Drug-efficacy Evaluation of Diabetic Kidney Disease in Rats |
| title_fullStr | Application of PNR Detection in the Diagnosis and Drug-efficacy Evaluation of Diabetic Kidney Disease in Rats |
| title_full_unstemmed | Application of PNR Detection in the Diagnosis and Drug-efficacy Evaluation of Diabetic Kidney Disease in Rats |
| title_short | Application of PNR Detection in the Diagnosis and Drug-efficacy Evaluation of Diabetic Kidney Disease in Rats |
| title_sort | application of pnr detection in the diagnosis and drug efficacy evaluation of diabetic kidney disease in rats |
| topic | pnr urinary albumin-to-creatinine ratio (u-acr) diabetic kidney disease proteinuria prevention rats |
| url | https://www.slarc.org.cn/dwyx/CN/10.12300/j.issn.1674-5817.2024.044 |
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