Genome-wide identification of NDR1/HIN1-like genes in kiwifruit and function analysis of AeNHL17 in response to disease resistance
Abstract Background NDR1/HIN1-like (NHL) genes play crucial roles in Psa resistance. Kiwifruit canker, caused by Pseudomonas syringae pv. Actinidiae (Psa) infection is one of the most serious diseases affecting the kiwifruit industry. However, the key NHL has not yet been identified in kiwifruit. Re...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-12-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-024-05936-2 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846112651265966080 |
|---|---|
| author | Min Zhang Rong Fu Miao-Miao Lin Jin-Bao Fang Ran Wang Yu-Kuo Li Jin-Yong Chen Lei-Ming Sun Xiu-Juan Qi |
| author_facet | Min Zhang Rong Fu Miao-Miao Lin Jin-Bao Fang Ran Wang Yu-Kuo Li Jin-Yong Chen Lei-Ming Sun Xiu-Juan Qi |
| author_sort | Min Zhang |
| collection | DOAJ |
| description | Abstract Background NDR1/HIN1-like (NHL) genes play crucial roles in Psa resistance. Kiwifruit canker, caused by Pseudomonas syringae pv. Actinidiae (Psa) infection is one of the most serious diseases affecting the kiwifruit industry. However, the key NHL has not yet been identified in kiwifruit. Results In this study, we conducted a genome-wide identification of NHL family in kiwifruit (Actinidia eriantha). A total of 33 AeNHLs were divided into five domain-conserved subfamilies, which were mainly assigned into phytohormones and defense responses. The expression of AeNHL genes was analyzed to identify key genes in response to Psa, and we found AeNHL17 was highly expressed upon Psa inoculation. Transgenic tobacco overexpressing AeNHL17 presented higher resistance to Psa than wild-type (WT) tobacco, implying a key role for AeNHL17 in Psa resistance. Finally, we carried out a stable genetic transformation of kiwifruit (A. chinensis), which is sensitive to Psa, and found that the overexpression of AeNHL17 increased resistance to infection. AeNHL17-silenced plants exhibited larger disease lesions than control plants. Conclusions Our findings revealed the function of AaNHL17 in Psa resistance, providing new data regarding the functional analysis of the NHL gene family in kiwifruit. |
| format | Article |
| id | doaj-art-a1c0e1868fdf477c83561624969054a8 |
| institution | Kabale University |
| issn | 1471-2229 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj-art-a1c0e1868fdf477c83561624969054a82024-12-22T12:25:01ZengBMCBMC Plant Biology1471-22292024-12-0124111710.1186/s12870-024-05936-2Genome-wide identification of NDR1/HIN1-like genes in kiwifruit and function analysis of AeNHL17 in response to disease resistanceMin Zhang0Rong Fu1Miao-Miao Lin2Jin-Bao Fang3Ran Wang4Yu-Kuo Li5Jin-Yong Chen6Lei-Ming Sun7Xiu-Juan Qi8National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop/ Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop/ Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop/ Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop/ Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop/ Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop/ Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop/ Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop/ Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesNational Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crop/ Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural SciencesAbstract Background NDR1/HIN1-like (NHL) genes play crucial roles in Psa resistance. Kiwifruit canker, caused by Pseudomonas syringae pv. Actinidiae (Psa) infection is one of the most serious diseases affecting the kiwifruit industry. However, the key NHL has not yet been identified in kiwifruit. Results In this study, we conducted a genome-wide identification of NHL family in kiwifruit (Actinidia eriantha). A total of 33 AeNHLs were divided into five domain-conserved subfamilies, which were mainly assigned into phytohormones and defense responses. The expression of AeNHL genes was analyzed to identify key genes in response to Psa, and we found AeNHL17 was highly expressed upon Psa inoculation. Transgenic tobacco overexpressing AeNHL17 presented higher resistance to Psa than wild-type (WT) tobacco, implying a key role for AeNHL17 in Psa resistance. Finally, we carried out a stable genetic transformation of kiwifruit (A. chinensis), which is sensitive to Psa, and found that the overexpression of AeNHL17 increased resistance to infection. AeNHL17-silenced plants exhibited larger disease lesions than control plants. Conclusions Our findings revealed the function of AaNHL17 in Psa resistance, providing new data regarding the functional analysis of the NHL gene family in kiwifruit.https://doi.org/10.1186/s12870-024-05936-2NDR1/HIN1-like gene familyKiwifruitPsaAeNHL17Disease resistance |
| spellingShingle | Min Zhang Rong Fu Miao-Miao Lin Jin-Bao Fang Ran Wang Yu-Kuo Li Jin-Yong Chen Lei-Ming Sun Xiu-Juan Qi Genome-wide identification of NDR1/HIN1-like genes in kiwifruit and function analysis of AeNHL17 in response to disease resistance BMC Plant Biology NDR1/HIN1-like gene family Kiwifruit Psa AeNHL17 Disease resistance |
| title | Genome-wide identification of NDR1/HIN1-like genes in kiwifruit and function analysis of AeNHL17 in response to disease resistance |
| title_full | Genome-wide identification of NDR1/HIN1-like genes in kiwifruit and function analysis of AeNHL17 in response to disease resistance |
| title_fullStr | Genome-wide identification of NDR1/HIN1-like genes in kiwifruit and function analysis of AeNHL17 in response to disease resistance |
| title_full_unstemmed | Genome-wide identification of NDR1/HIN1-like genes in kiwifruit and function analysis of AeNHL17 in response to disease resistance |
| title_short | Genome-wide identification of NDR1/HIN1-like genes in kiwifruit and function analysis of AeNHL17 in response to disease resistance |
| title_sort | genome wide identification of ndr1 hin1 like genes in kiwifruit and function analysis of aenhl17 in response to disease resistance |
| topic | NDR1/HIN1-like gene family Kiwifruit Psa AeNHL17 Disease resistance |
| url | https://doi.org/10.1186/s12870-024-05936-2 |
| work_keys_str_mv | AT minzhang genomewideidentificationofndr1hin1likegenesinkiwifruitandfunctionanalysisofaenhl17inresponsetodiseaseresistance AT rongfu genomewideidentificationofndr1hin1likegenesinkiwifruitandfunctionanalysisofaenhl17inresponsetodiseaseresistance AT miaomiaolin genomewideidentificationofndr1hin1likegenesinkiwifruitandfunctionanalysisofaenhl17inresponsetodiseaseresistance AT jinbaofang genomewideidentificationofndr1hin1likegenesinkiwifruitandfunctionanalysisofaenhl17inresponsetodiseaseresistance AT ranwang genomewideidentificationofndr1hin1likegenesinkiwifruitandfunctionanalysisofaenhl17inresponsetodiseaseresistance AT yukuoli genomewideidentificationofndr1hin1likegenesinkiwifruitandfunctionanalysisofaenhl17inresponsetodiseaseresistance AT jinyongchen genomewideidentificationofndr1hin1likegenesinkiwifruitandfunctionanalysisofaenhl17inresponsetodiseaseresistance AT leimingsun genomewideidentificationofndr1hin1likegenesinkiwifruitandfunctionanalysisofaenhl17inresponsetodiseaseresistance AT xiujuanqi genomewideidentificationofndr1hin1likegenesinkiwifruitandfunctionanalysisofaenhl17inresponsetodiseaseresistance |