EBV Reactivation and Chromosomal Polysomies: Euphorbia tirucalli as a Possible Cofactor in Endemic Burkitt Lymphoma
Burkitt lymphoma is endemic in the Equatorial Belt of Africa, its molecular hallmark is an activated, MYC gene mostly due to a chromosomal translocation. Especially in its endemic clinical variant, Burkitt lymphoma is associated with the oncogenic Epstein-Barr virus (EBV), and holoendemic malaria ac...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Advances in Hematology |
| Online Access: | http://dx.doi.org/10.1155/2012/149780 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849398511712337920 |
|---|---|
| author | Susanna Mannucci Anna Luzzi Alessandro Carugi Alessandro Gozzetti Stefano Lazzi Valeria Malagnino Monique Simmonds Maria Grazia Cusi Lorenzo Leoncini Cornelia A. van den Bosch Giulia De Falco |
| author_facet | Susanna Mannucci Anna Luzzi Alessandro Carugi Alessandro Gozzetti Stefano Lazzi Valeria Malagnino Monique Simmonds Maria Grazia Cusi Lorenzo Leoncini Cornelia A. van den Bosch Giulia De Falco |
| author_sort | Susanna Mannucci |
| collection | DOAJ |
| description | Burkitt lymphoma is endemic in the Equatorial Belt of Africa, its molecular hallmark is an activated, MYC gene mostly due to a chromosomal translocation. Especially in its endemic clinical variant, Burkitt lymphoma is associated with the oncogenic Epstein-Barr virus (EBV), and holoendemic malaria acts as an amplifier. Environmental factors may also cooperate in Burkitt lymphomagenesis in the endemic regions, such as plants used as traditional herbal remedies. Euphorbia tirucalli, a plant known to possess EBV-activating substances, has a similar geographical distribution to endemic Burkitt’s Lymphoma and is used as a hedge, herbal remedy and toy in the Lymphoma BeltI. In this study we aimed at determining if exposure to Euphorbia tirucalli could contribute to lymphomagenesis, and at which extent. Lymphoblastoid and cord blood-derived cell lines were treated with plant extracts, and the expression of EBV-coded proteins was checked, to assess EBV reactivation. The occurrence of chromosomal translocations was then investigated by FISH. Our preliminary results suggest that E. tirucalli is able to reactivate EBV and determine chromosomal alterations, which leads to c-MYC altered expression. The existence of genomic alterations might determine the accumulation of further genetic alteration, which could eventually lead to a transformed phenotype. |
| format | Article |
| id | doaj-art-a1b78fc1e1d545859c5e576cdc9eb9af |
| institution | Kabale University |
| issn | 1687-9104 1687-9112 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Hematology |
| spelling | doaj-art-a1b78fc1e1d545859c5e576cdc9eb9af2025-08-20T03:38:35ZengWileyAdvances in Hematology1687-91041687-91122012-01-01201210.1155/2012/149780149780EBV Reactivation and Chromosomal Polysomies: Euphorbia tirucalli as a Possible Cofactor in Endemic Burkitt LymphomaSusanna Mannucci0Anna Luzzi1Alessandro Carugi2Alessandro Gozzetti3Stefano Lazzi4Valeria Malagnino5Monique Simmonds6Maria Grazia Cusi7Lorenzo Leoncini8Cornelia A. van den Bosch9Giulia De Falco10Department Human Pathology and Oncology, University of Siena, 53100 Siena, ItalyDepartment Human Pathology and Oncology, University of Siena, 53100 Siena, ItalyDepartment Human Pathology and Oncology, University of Siena, 53100 Siena, ItalyDepartment of Oncology, Hematology, University of Siena, 53100 Siena, ItalyDepartment Human Pathology and Oncology, University of Siena, 53100 Siena, ItalyDepartment Human Pathology and Oncology, University of Siena, 53100 Siena, ItalyPilgrims' Hospice, Margate, Kent CT94AD, UKDepartment Biotechnology, Section of Microbiology, University of Siena, 53100 Siena, ItalyDepartment Human Pathology and Oncology, University of Siena, 53100 Siena, ItalyPilgrims' Hospice, Margate, Kent CT94AD, UKDepartment Human Pathology and Oncology, University of Siena, 53100 Siena, ItalyBurkitt lymphoma is endemic in the Equatorial Belt of Africa, its molecular hallmark is an activated, MYC gene mostly due to a chromosomal translocation. Especially in its endemic clinical variant, Burkitt lymphoma is associated with the oncogenic Epstein-Barr virus (EBV), and holoendemic malaria acts as an amplifier. Environmental factors may also cooperate in Burkitt lymphomagenesis in the endemic regions, such as plants used as traditional herbal remedies. Euphorbia tirucalli, a plant known to possess EBV-activating substances, has a similar geographical distribution to endemic Burkitt’s Lymphoma and is used as a hedge, herbal remedy and toy in the Lymphoma BeltI. In this study we aimed at determining if exposure to Euphorbia tirucalli could contribute to lymphomagenesis, and at which extent. Lymphoblastoid and cord blood-derived cell lines were treated with plant extracts, and the expression of EBV-coded proteins was checked, to assess EBV reactivation. The occurrence of chromosomal translocations was then investigated by FISH. Our preliminary results suggest that E. tirucalli is able to reactivate EBV and determine chromosomal alterations, which leads to c-MYC altered expression. The existence of genomic alterations might determine the accumulation of further genetic alteration, which could eventually lead to a transformed phenotype.http://dx.doi.org/10.1155/2012/149780 |
| spellingShingle | Susanna Mannucci Anna Luzzi Alessandro Carugi Alessandro Gozzetti Stefano Lazzi Valeria Malagnino Monique Simmonds Maria Grazia Cusi Lorenzo Leoncini Cornelia A. van den Bosch Giulia De Falco EBV Reactivation and Chromosomal Polysomies: Euphorbia tirucalli as a Possible Cofactor in Endemic Burkitt Lymphoma Advances in Hematology |
| title | EBV Reactivation and Chromosomal Polysomies: Euphorbia tirucalli as a Possible Cofactor in Endemic Burkitt Lymphoma |
| title_full | EBV Reactivation and Chromosomal Polysomies: Euphorbia tirucalli as a Possible Cofactor in Endemic Burkitt Lymphoma |
| title_fullStr | EBV Reactivation and Chromosomal Polysomies: Euphorbia tirucalli as a Possible Cofactor in Endemic Burkitt Lymphoma |
| title_full_unstemmed | EBV Reactivation and Chromosomal Polysomies: Euphorbia tirucalli as a Possible Cofactor in Endemic Burkitt Lymphoma |
| title_short | EBV Reactivation and Chromosomal Polysomies: Euphorbia tirucalli as a Possible Cofactor in Endemic Burkitt Lymphoma |
| title_sort | ebv reactivation and chromosomal polysomies euphorbia tirucalli as a possible cofactor in endemic burkitt lymphoma |
| url | http://dx.doi.org/10.1155/2012/149780 |
| work_keys_str_mv | AT susannamannucci ebvreactivationandchromosomalpolysomieseuphorbiatirucalliasapossiblecofactorinendemicburkittlymphoma AT annaluzzi ebvreactivationandchromosomalpolysomieseuphorbiatirucalliasapossiblecofactorinendemicburkittlymphoma AT alessandrocarugi ebvreactivationandchromosomalpolysomieseuphorbiatirucalliasapossiblecofactorinendemicburkittlymphoma AT alessandrogozzetti ebvreactivationandchromosomalpolysomieseuphorbiatirucalliasapossiblecofactorinendemicburkittlymphoma AT stefanolazzi ebvreactivationandchromosomalpolysomieseuphorbiatirucalliasapossiblecofactorinendemicburkittlymphoma AT valeriamalagnino ebvreactivationandchromosomalpolysomieseuphorbiatirucalliasapossiblecofactorinendemicburkittlymphoma AT moniquesimmonds ebvreactivationandchromosomalpolysomieseuphorbiatirucalliasapossiblecofactorinendemicburkittlymphoma AT mariagraziacusi ebvreactivationandchromosomalpolysomieseuphorbiatirucalliasapossiblecofactorinendemicburkittlymphoma AT lorenzoleoncini ebvreactivationandchromosomalpolysomieseuphorbiatirucalliasapossiblecofactorinendemicburkittlymphoma AT corneliaavandenbosch ebvreactivationandchromosomalpolysomieseuphorbiatirucalliasapossiblecofactorinendemicburkittlymphoma AT giuliadefalco ebvreactivationandchromosomalpolysomieseuphorbiatirucalliasapossiblecofactorinendemicburkittlymphoma |