LncRNA MIR210HG promotes the proliferation of colon cancer cells by inhibiting ferroptosis through binding to PCBP1
Abstract This study aimed to investigate the role of the MIR210 host gene (MIR210HG), a long noncoding RNA (lncRNA), in the proliferation of colon cancer cells and its potential mechanism involving the ferroptosis pathway. We assessed MIR210HG expression in colon cancer cell lines and tissues, and e...
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-85321-7 |
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| author | Xiao-Qian Wang A.-Qiang Fan Liu Hong |
| author_facet | Xiao-Qian Wang A.-Qiang Fan Liu Hong |
| author_sort | Xiao-Qian Wang |
| collection | DOAJ |
| description | Abstract This study aimed to investigate the role of the MIR210 host gene (MIR210HG), a long noncoding RNA (lncRNA), in the proliferation of colon cancer cells and its potential mechanism involving the ferroptosis pathway. We assessed MIR210HG expression in colon cancer cell lines and tissues, and examined the effects of its overexpression and knockdown on cell proliferation. Proteomic analysis was conducted to explore the interaction between MIR210HG and ferroptosis pathway components. The binding of MIR210HG to poly(rC) binding protein 1 (PCBP1) was predicted using catRAPID and confirmed through RNA pull-down and RNA immunoprecipitation (RIP) experiments. MIR210HG was significantly upregulated in colon cancer cells and tissues. Its overexpression promoted, while its knockdown inhibited, colon cancer cell proliferation. MIR210HG was found to be associated with ferroptosis pathway components and to bind to PCBP1, which was experimentally validated. The inhibition of ferroptosis by MIR210HG through PCBP1 binding was confirmed, highlighting its role in promoting cell proliferation. MIR210HG promotes colon cancer cell proliferation by binding to PCBP1 and inhibiting ferroptosis. These findings suggest MIR210HG as a potential therapeutic target for colon cancer. |
| format | Article |
| id | doaj-art-a17528e55a754568914cc791467017bd |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-a17528e55a754568914cc791467017bd2025-01-12T12:23:03ZengNature PortfolioScientific Reports2045-23222025-01-0115111110.1038/s41598-025-85321-7LncRNA MIR210HG promotes the proliferation of colon cancer cells by inhibiting ferroptosis through binding to PCBP1Xiao-Qian Wang0A.-Qiang Fan1Liu Hong2Department of Digestive Surgery, Xijing Hospital of Digestive Diseases, Air Force Medical UniversityDepartment of Digestive Surgery, Xijing Hospital of Digestive Diseases, Air Force Medical UniversityDepartment of Digestive Surgery, Xijing Hospital of Digestive Diseases, Air Force Medical UniversityAbstract This study aimed to investigate the role of the MIR210 host gene (MIR210HG), a long noncoding RNA (lncRNA), in the proliferation of colon cancer cells and its potential mechanism involving the ferroptosis pathway. We assessed MIR210HG expression in colon cancer cell lines and tissues, and examined the effects of its overexpression and knockdown on cell proliferation. Proteomic analysis was conducted to explore the interaction between MIR210HG and ferroptosis pathway components. The binding of MIR210HG to poly(rC) binding protein 1 (PCBP1) was predicted using catRAPID and confirmed through RNA pull-down and RNA immunoprecipitation (RIP) experiments. MIR210HG was significantly upregulated in colon cancer cells and tissues. Its overexpression promoted, while its knockdown inhibited, colon cancer cell proliferation. MIR210HG was found to be associated with ferroptosis pathway components and to bind to PCBP1, which was experimentally validated. The inhibition of ferroptosis by MIR210HG through PCBP1 binding was confirmed, highlighting its role in promoting cell proliferation. MIR210HG promotes colon cancer cell proliferation by binding to PCBP1 and inhibiting ferroptosis. These findings suggest MIR210HG as a potential therapeutic target for colon cancer.https://doi.org/10.1038/s41598-025-85321-7Long noncoding RNAFerroptosisPCBP1Colon cancerProliferationMIR210HG |
| spellingShingle | Xiao-Qian Wang A.-Qiang Fan Liu Hong LncRNA MIR210HG promotes the proliferation of colon cancer cells by inhibiting ferroptosis through binding to PCBP1 Scientific Reports Long noncoding RNA Ferroptosis PCBP1 Colon cancer Proliferation MIR210HG |
| title | LncRNA MIR210HG promotes the proliferation of colon cancer cells by inhibiting ferroptosis through binding to PCBP1 |
| title_full | LncRNA MIR210HG promotes the proliferation of colon cancer cells by inhibiting ferroptosis through binding to PCBP1 |
| title_fullStr | LncRNA MIR210HG promotes the proliferation of colon cancer cells by inhibiting ferroptosis through binding to PCBP1 |
| title_full_unstemmed | LncRNA MIR210HG promotes the proliferation of colon cancer cells by inhibiting ferroptosis through binding to PCBP1 |
| title_short | LncRNA MIR210HG promotes the proliferation of colon cancer cells by inhibiting ferroptosis through binding to PCBP1 |
| title_sort | lncrna mir210hg promotes the proliferation of colon cancer cells by inhibiting ferroptosis through binding to pcbp1 |
| topic | Long noncoding RNA Ferroptosis PCBP1 Colon cancer Proliferation MIR210HG |
| url | https://doi.org/10.1038/s41598-025-85321-7 |
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