Adenine base editors induce off-target structure variations in mouse embryos and primary human T cells

Adenine base editors induce off-target structure variations in mouse embryos and primary human T cells

Abstract Background The safety of CRISPR-based gene editing methods is of the utmost priority in clinical applications. Previous studies have reported that Cas9 cleavage induced frequent aneuploidy in primary human T cells, but whether cleavage-mediated editing of base editors would generate off-tar...

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Bibliographic Details
Main Authors: Leilei Wu, Shutan Jiang, Meisong Shi, Tanglong Yuan, Yaqin Li, Pinzheng Huang, Yingqi Li, Erwei Zuo, Changyang Zhou, Yidi Sun
Format: Article
Language:English
Published: BMC 2024-11-01
Series:Genome Biology
Subjects:
ABE
Cas9
Off-target structure variation
Large deletion
T cell
Online Access:https://doi.org/10.1186/s13059-024-03434-0
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