Diversity and antimicrobial resistance profiles of Mycobacterium avium complex clinical isolates in Thailand based on whole genome comparative analysis
Abstract The Mycobacterium avium complex (MAC) is a group of closely related nontuberculous mycobacteria that can cause various diseases in humans. In this study, genome sequencing, comprehensive genomic analysis, and antimicrobial susceptibility testing of 66 MAC clinical isolates from King Chulalo...
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Nature Portfolio
2025-01-01
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| author | Vorthon Sawaswong Kanphai Wongjarit Suthidee Petsong Yonita Yuliani Ubonwan Somsukpiroh Kiatichai Faksri Taya Forde Sunchai Payungporn Suwatchareeporn Rotcheewaphan |
| author_facet | Vorthon Sawaswong Kanphai Wongjarit Suthidee Petsong Yonita Yuliani Ubonwan Somsukpiroh Kiatichai Faksri Taya Forde Sunchai Payungporn Suwatchareeporn Rotcheewaphan |
| author_sort | Vorthon Sawaswong |
| collection | DOAJ |
| description | Abstract The Mycobacterium avium complex (MAC) is a group of closely related nontuberculous mycobacteria that can cause various diseases in humans. In this study, genome sequencing, comprehensive genomic analysis, and antimicrobial susceptibility testing of 66 MAC clinical isolates from King Chulalongkorn Memorial Hospital, Bangkok, Thailand were carried out. Whole-genome average nucleotide identity (ANI) revealed the MAC species distribution, comprising 54 (81.8%) M. intracellulare, 6 (9.1%) M. avium, 5 (7.6%) M. colombiense, and 1 (1.5%) M. timonense. Phylogenetic analysis revealed a high diversity of M. intracellulare isolates and their evolutionary relationships which could be divided into 2 subspecies: M. intracellulare subsp. intracellulare and M. intracellulare subsp. chimaera. In addition, M. intracellulare subsp. chimaera mostly clustered in the distinct clades separated from M. intracellulare strains originating from other countries. Most MAC isolates were resistant to linezolid and moxifloxacin based on phenotypic antimicrobial susceptibility testing. Mutations within rrl gene associated with clarithromycin resistance were detected in M. intracellulare and M. colombiense. The pan-genome analysis presented clade-specific proteins for M. intracellulare, such as PE and PPE protein families. This study provides valuable insights into the genomic diversity and antimicrobial resistance profiles of MAC isolates circulating in Thailand, which are useful for clinical management, guiding the development of targeted diagnostic, and treatment strategies for MAC infections. |
| format | Article |
| id | doaj-art-a0c976b0c97b45a5bf2998946b87058c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-a0c976b0c97b45a5bf2998946b87058c2025-01-05T12:22:32ZengNature PortfolioScientific Reports2045-23222025-01-0115111410.1038/s41598-024-84511-zDiversity and antimicrobial resistance profiles of Mycobacterium avium complex clinical isolates in Thailand based on whole genome comparative analysisVorthon Sawaswong0Kanphai Wongjarit1Suthidee Petsong2Yonita Yuliani3Ubonwan Somsukpiroh4Kiatichai Faksri5Taya Forde6Sunchai Payungporn7Suwatchareeporn Rotcheewaphan8Department of Biochemistry, Faculty of Science, Mahidol UniversityDepartment of Microbiology, Faculty of Medicine, Chulalongkorn UniversityDepartment of Microbiology, Faculty of Medicine, Chulalongkorn UniversityMedical Sciences, Faculty of Medicine, Chulalongkorn UniversityDepartment of Microbiology, King Chulalongkorn Memorial HospitalDepartment of Microbiology, Faculty of Medicine, Khon Kaen UniversitySchool of Biodiversity, One Health & Veterinary Medicine, University of GlasgowDepartment of Biochemistry, Faculty of Medicine, Chulalongkorn UniversityDepartment of Microbiology, Faculty of Medicine, Chulalongkorn UniversityAbstract The Mycobacterium avium complex (MAC) is a group of closely related nontuberculous mycobacteria that can cause various diseases in humans. In this study, genome sequencing, comprehensive genomic analysis, and antimicrobial susceptibility testing of 66 MAC clinical isolates from King Chulalongkorn Memorial Hospital, Bangkok, Thailand were carried out. Whole-genome average nucleotide identity (ANI) revealed the MAC species distribution, comprising 54 (81.8%) M. intracellulare, 6 (9.1%) M. avium, 5 (7.6%) M. colombiense, and 1 (1.5%) M. timonense. Phylogenetic analysis revealed a high diversity of M. intracellulare isolates and their evolutionary relationships which could be divided into 2 subspecies: M. intracellulare subsp. intracellulare and M. intracellulare subsp. chimaera. In addition, M. intracellulare subsp. chimaera mostly clustered in the distinct clades separated from M. intracellulare strains originating from other countries. Most MAC isolates were resistant to linezolid and moxifloxacin based on phenotypic antimicrobial susceptibility testing. Mutations within rrl gene associated with clarithromycin resistance were detected in M. intracellulare and M. colombiense. The pan-genome analysis presented clade-specific proteins for M. intracellulare, such as PE and PPE protein families. This study provides valuable insights into the genomic diversity and antimicrobial resistance profiles of MAC isolates circulating in Thailand, which are useful for clinical management, guiding the development of targeted diagnostic, and treatment strategies for MAC infections.https://doi.org/10.1038/s41598-024-84511-zMycobacterium avium complexWhole genome sequenceAntimicrobial susceptibility testingWhole genome comparative analysis |
| spellingShingle | Vorthon Sawaswong Kanphai Wongjarit Suthidee Petsong Yonita Yuliani Ubonwan Somsukpiroh Kiatichai Faksri Taya Forde Sunchai Payungporn Suwatchareeporn Rotcheewaphan Diversity and antimicrobial resistance profiles of Mycobacterium avium complex clinical isolates in Thailand based on whole genome comparative analysis Scientific Reports Mycobacterium avium complex Whole genome sequence Antimicrobial susceptibility testing Whole genome comparative analysis |
| title | Diversity and antimicrobial resistance profiles of Mycobacterium avium complex clinical isolates in Thailand based on whole genome comparative analysis |
| title_full | Diversity and antimicrobial resistance profiles of Mycobacterium avium complex clinical isolates in Thailand based on whole genome comparative analysis |
| title_fullStr | Diversity and antimicrobial resistance profiles of Mycobacterium avium complex clinical isolates in Thailand based on whole genome comparative analysis |
| title_full_unstemmed | Diversity and antimicrobial resistance profiles of Mycobacterium avium complex clinical isolates in Thailand based on whole genome comparative analysis |
| title_short | Diversity and antimicrobial resistance profiles of Mycobacterium avium complex clinical isolates in Thailand based on whole genome comparative analysis |
| title_sort | diversity and antimicrobial resistance profiles of mycobacterium avium complex clinical isolates in thailand based on whole genome comparative analysis |
| topic | Mycobacterium avium complex Whole genome sequence Antimicrobial susceptibility testing Whole genome comparative analysis |
| url | https://doi.org/10.1038/s41598-024-84511-z |
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