Rapid and Robust Generation of Homozygous Fluorescent Reporter Knock-In Cell Pools by CRISPR-Cas9

Conventional methods for generating knock-out or knock-in mammalian cell models using CRISPR-Cas9 genome editing often require tedious single-cell clone selection and expansion. In this study, we develop and optimise rapid and robust strategies to engineer homozygous fluorescent reporter knock-in ce...

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Bibliographic Details
Main Authors:	Jicheng Yang, Fusheng Guo, Hui San Chin, Gao Bin Chen, Ziyan Zhang, Lewis Williams, Andrew J. Kueh, Pierce K. H. Chow, Marco J. Herold, Nai Yang Fu
Format:	Article
Language:	English
Published:	MDPI AG 2025-07-01
Series:	Cells
Subjects:	genome editing CRISPR-Cas9 biallelic editing CRISPR screen genetically engineered cells fluorescent reporter knock-in cells
Online Access:	https://www.mdpi.com/2073-4409/14/15/1165
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Rapid and Robust Generation of Homozygous Fluorescent Reporter Knock-In Cell Pools by CRISPR-Cas9

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