Multimodal Imaging of Neural Progenitor Cell Fate in Rodents
For clinical application of stem cell–based therapies, noninvasive detection of applied stem cells is of high importance. We report on the feasibility of detecting implanted neural progenitor cells (NPCs) noninvasively and follow their fate and functional status by sequential multimodal molecular im...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2008-03-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2008.0010 |
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| _version_ | 1841561722767278080 |
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| author | Yannic Waerzeggers Markus Klein Hrvoje Miletic Uwe Himmelreich Hongfeng Li Parisa Monfared Ulrich Herrlinger Mathias Hoehn Heinrich Hubert Coenen Michael Weller Alexandra Winkeler Andreas Hans Jacobs |
| author_facet | Yannic Waerzeggers Markus Klein Hrvoje Miletic Uwe Himmelreich Hongfeng Li Parisa Monfared Ulrich Herrlinger Mathias Hoehn Heinrich Hubert Coenen Michael Weller Alexandra Winkeler Andreas Hans Jacobs |
| author_sort | Yannic Waerzeggers |
| collection | DOAJ |
| description | For clinical application of stem cell–based therapies, noninvasive detection of applied stem cells is of high importance. We report on the feasibility of detecting implanted neural progenitor cells (NPCs) noninvasively and follow their fate and functional status by sequential multimodal molecular imaging and reporter gene technology. We investigated C17.2 cells stably expressing herpes simplex virus type 1–thymidine kinase (HSV-1- tk ) and green fluorescent protein ( gfp ) (C17.2- tk IRES gfp = C17.2-TIG) or HSV-1- tk , gfp , and firefly luciferase ( luc ) (C17.2- luc IRES tkgfp = C17.2-LITG) and determined the detection sensitivity of positron emission tomography (PET) and bioluminescence imaging (BLI) for these cells in culture and in vivo in subcutaneous and intracranial glioma models. In addition, PET and BLI were used to further investigate and follow the fate of implanted C17.2-LITG cells in an intracranial glioma model. We show that both imaging modalities are sensitive in detecting reporter gene expressing NPCs; however, PET, by the use of 9-[4-[ 18 F]fluoro-3-hydroxymethyl)butyl]guanine ([ 18 F]FHBG), detects NPCs only at sites of disrupted blood-brain barrier. Furthermore, both imaging modalities can be used to detect stem cell fate and migration and indicate excessive proliferation and aberrant migration. In conclusion, multimodal imaging can be used for longitudinal noninvasive monitoring of grafted NPCs in rodents. |
| format | Article |
| id | doaj-art-a05c17795fc7420f8d40f49ebab3c678 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2008-03-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-a05c17795fc7420f8d40f49ebab3c6782025-01-03T01:25:16ZengSAGE PublishingMolecular Imaging1536-01212008-03-01710.2310/7290.2008.001010.2310_7290.2008.0010Multimodal Imaging of Neural Progenitor Cell Fate in RodentsYannic WaerzeggersMarkus KleinHrvoje MileticUwe HimmelreichHongfeng LiParisa MonfaredUlrich HerrlingerMathias HoehnHeinrich Hubert CoenenMichael WellerAlexandra WinkelerAndreas Hans JacobsFor clinical application of stem cell–based therapies, noninvasive detection of applied stem cells is of high importance. We report on the feasibility of detecting implanted neural progenitor cells (NPCs) noninvasively and follow their fate and functional status by sequential multimodal molecular imaging and reporter gene technology. We investigated C17.2 cells stably expressing herpes simplex virus type 1–thymidine kinase (HSV-1- tk ) and green fluorescent protein ( gfp ) (C17.2- tk IRES gfp = C17.2-TIG) or HSV-1- tk , gfp , and firefly luciferase ( luc ) (C17.2- luc IRES tkgfp = C17.2-LITG) and determined the detection sensitivity of positron emission tomography (PET) and bioluminescence imaging (BLI) for these cells in culture and in vivo in subcutaneous and intracranial glioma models. In addition, PET and BLI were used to further investigate and follow the fate of implanted C17.2-LITG cells in an intracranial glioma model. We show that both imaging modalities are sensitive in detecting reporter gene expressing NPCs; however, PET, by the use of 9-[4-[ 18 F]fluoro-3-hydroxymethyl)butyl]guanine ([ 18 F]FHBG), detects NPCs only at sites of disrupted blood-brain barrier. Furthermore, both imaging modalities can be used to detect stem cell fate and migration and indicate excessive proliferation and aberrant migration. In conclusion, multimodal imaging can be used for longitudinal noninvasive monitoring of grafted NPCs in rodents.https://doi.org/10.2310/7290.2008.0010 |
| spellingShingle | Yannic Waerzeggers Markus Klein Hrvoje Miletic Uwe Himmelreich Hongfeng Li Parisa Monfared Ulrich Herrlinger Mathias Hoehn Heinrich Hubert Coenen Michael Weller Alexandra Winkeler Andreas Hans Jacobs Multimodal Imaging of Neural Progenitor Cell Fate in Rodents Molecular Imaging |
| title | Multimodal Imaging of Neural Progenitor Cell Fate in Rodents |
| title_full | Multimodal Imaging of Neural Progenitor Cell Fate in Rodents |
| title_fullStr | Multimodal Imaging of Neural Progenitor Cell Fate in Rodents |
| title_full_unstemmed | Multimodal Imaging of Neural Progenitor Cell Fate in Rodents |
| title_short | Multimodal Imaging of Neural Progenitor Cell Fate in Rodents |
| title_sort | multimodal imaging of neural progenitor cell fate in rodents |
| url | https://doi.org/10.2310/7290.2008.0010 |
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