Computational-guided discovery of UDP-glycosyltransferases for lauryl glucoside production using engineered E. coli

Computational-guided discovery of UDP-glycosyltransferases for lauryl glucoside production using engineered E. coli

Abstract Defining suitable enzymes for reaction steps in novel synthetic pathways is crucial for developing microbial cell factories for non-natural products. Here, we developed a computational workflow to identify C12 alcohol-active UDP-glycosyltransferases. The workflow involved three steps: (1) a...

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Bibliographic Details
Main Authors: Kasimaporn Promubon, Kritsada Tathiya, Aussara Panya, Wasu Pathom-Aree, Pachara Sattayawat
Format: Article
Language:English
Published: SpringerOpen 2024-10-01
Series:Bioresources and Bioprocessing
Subjects:
UDP-glycosyltransferase
Lauryl glucoside
Computational method
1-Dodecanol
Engineered E. coli
Novel biosynthetic pathway
Online Access:https://doi.org/10.1186/s40643-024-00820-1
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