Microfluidic Nanobioplatform-Based Immunosensor for Monitoring of 25-Hydroxy Vitamin-D3
Measurement of 25-hydroxyvitamin-D _3 (25-VitD _3 ) is crucial because its deficiency is linked to a variety of disorders, including osteoporosis, diabetes, depression, some autoimmune conditions, and even Covid-19. Hence, it is important to find new methods for efficient rapid testing for clinical...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
IOP Publishing
2024-01-01
|
| Series: | ECS Sensors Plus |
| Subjects: | |
| Online Access: | https://doi.org/10.1149/2754-2726/ad9935 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846120086287417344 |
|---|---|
| author | Deepika Chauhan Zimad Hashmi Rishi Raj Pratima R. Solanki |
| author_facet | Deepika Chauhan Zimad Hashmi Rishi Raj Pratima R. Solanki |
| author_sort | Deepika Chauhan |
| collection | DOAJ |
| description | Measurement of 25-hydroxyvitamin-D _3 (25-VitD _3 ) is crucial because its deficiency is linked to a variety of disorders, including osteoporosis, diabetes, depression, some autoimmune conditions, and even Covid-19. Hence, it is important to find new methods for efficient rapid testing for clinical diagnosis and public health prevention. Here, a new nanomaterial-based microfluidic electrochemical immunosensor towards the determination of 25-VitD _3 using microwave synthesized gadolinium oxide nanoparticles (GdNPs) is presented. Mask-less lithography was used to create a micro-fluidic nano-bioplatform (MNBP) and three-electrode immunosensor on a transparent indium tin oxide (ITO) coated glass substrate. To fabricate MNBP (BSA/AB-25VitD _3 /CS@GdNPs/ITO), the working electrodes were functionalized with GdNPs, AB-25VitD _3 , and BSA (bovine serum albumin) in that order. The electrochemical response induced by the immunocomplex was measured in individual 25-VitD _3 concentrations (1–100 ng ml ^−1 ). This immunosensor exhibited a higher sensitivity of 5.99 ng ml ^−1 , limits of detection of 4.5 ng ml ^−1 , and an analysis time of 30 min. In addition, clinical serum samples from three healthy people were used to validate the MNBP-based immunosensor, which showed a strong correlation with traditional enzyme-linked immunosorbent assays. The use of proposed immunosensor to serum samples would allow early detection of 25-VitD _3 deficiency, particularly in rural and resource-constrained settings. |
| format | Article |
| id | doaj-art-9f7fbd9e5fe749a2a6d0b4d451119fa3 |
| institution | Kabale University |
| issn | 2754-2726 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | ECS Sensors Plus |
| spelling | doaj-art-9f7fbd9e5fe749a2a6d0b4d451119fa32024-12-16T13:34:50ZengIOP PublishingECS Sensors Plus2754-27262024-01-013404160110.1149/2754-2726/ad9935Microfluidic Nanobioplatform-Based Immunosensor for Monitoring of 25-Hydroxy Vitamin-D3Deepika Chauhan0https://orcid.org/0000-0002-1129-6578Zimad Hashmi1Rishi Raj2Pratima R. Solanki3https://orcid.org/0000-0003-1679-2180Special Centre for Nanoscience, Jawaharlal Nehru University , New Delhi-110067, IndiaSpecial Centre for Nanoscience, Jawaharlal Nehru University , New Delhi-110067, IndiaSpecial Centre for Nanoscience, Jawaharlal Nehru University , New Delhi-110067, IndiaSpecial Centre for Nanoscience, Jawaharlal Nehru University , New Delhi-110067, IndiaMeasurement of 25-hydroxyvitamin-D _3 (25-VitD _3 ) is crucial because its deficiency is linked to a variety of disorders, including osteoporosis, diabetes, depression, some autoimmune conditions, and even Covid-19. Hence, it is important to find new methods for efficient rapid testing for clinical diagnosis and public health prevention. Here, a new nanomaterial-based microfluidic electrochemical immunosensor towards the determination of 25-VitD _3 using microwave synthesized gadolinium oxide nanoparticles (GdNPs) is presented. Mask-less lithography was used to create a micro-fluidic nano-bioplatform (MNBP) and three-electrode immunosensor on a transparent indium tin oxide (ITO) coated glass substrate. To fabricate MNBP (BSA/AB-25VitD _3 /CS@GdNPs/ITO), the working electrodes were functionalized with GdNPs, AB-25VitD _3 , and BSA (bovine serum albumin) in that order. The electrochemical response induced by the immunocomplex was measured in individual 25-VitD _3 concentrations (1–100 ng ml ^−1 ). This immunosensor exhibited a higher sensitivity of 5.99 ng ml ^−1 , limits of detection of 4.5 ng ml ^−1 , and an analysis time of 30 min. In addition, clinical serum samples from three healthy people were used to validate the MNBP-based immunosensor, which showed a strong correlation with traditional enzyme-linked immunosorbent assays. The use of proposed immunosensor to serum samples would allow early detection of 25-VitD _3 deficiency, particularly in rural and resource-constrained settings.https://doi.org/10.1149/2754-2726/ad9935microfluidic immunosensorelectrochemicalGd NPs25-hydroxy vitamin-D3serum sample |
| spellingShingle | Deepika Chauhan Zimad Hashmi Rishi Raj Pratima R. Solanki Microfluidic Nanobioplatform-Based Immunosensor for Monitoring of 25-Hydroxy Vitamin-D3 ECS Sensors Plus microfluidic immunosensor electrochemical Gd NPs 25-hydroxy vitamin-D3 serum sample |
| title | Microfluidic Nanobioplatform-Based Immunosensor for Monitoring of 25-Hydroxy Vitamin-D3 |
| title_full | Microfluidic Nanobioplatform-Based Immunosensor for Monitoring of 25-Hydroxy Vitamin-D3 |
| title_fullStr | Microfluidic Nanobioplatform-Based Immunosensor for Monitoring of 25-Hydroxy Vitamin-D3 |
| title_full_unstemmed | Microfluidic Nanobioplatform-Based Immunosensor for Monitoring of 25-Hydroxy Vitamin-D3 |
| title_short | Microfluidic Nanobioplatform-Based Immunosensor for Monitoring of 25-Hydroxy Vitamin-D3 |
| title_sort | microfluidic nanobioplatform based immunosensor for monitoring of 25 hydroxy vitamin d3 |
| topic | microfluidic immunosensor electrochemical Gd NPs 25-hydroxy vitamin-D3 serum sample |
| url | https://doi.org/10.1149/2754-2726/ad9935 |
| work_keys_str_mv | AT deepikachauhan microfluidicnanobioplatformbasedimmunosensorformonitoringof25hydroxyvitamind3 AT zimadhashmi microfluidicnanobioplatformbasedimmunosensorformonitoringof25hydroxyvitamind3 AT rishiraj microfluidicnanobioplatformbasedimmunosensorformonitoringof25hydroxyvitamind3 AT pratimarsolanki microfluidicnanobioplatformbasedimmunosensorformonitoringof25hydroxyvitamind3 |