Analysis of Candidate miRNAs' Expression in Pancreatic Cancer
ABSTRACT Background Pancreatic cancer (PC) is one of the most aggressive types of cancer. Despite advances in molecular diagnostics, PC diagnosis relies on imaging technologies and morphological assessment of fine needle aspirates (FNAs). MicroRNA (miRNA) involvement in PC pathogenesis and potential...
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| Format: | Article |
| Language: | English |
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Wiley
2024-11-01
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| Series: | Cancer Medicine |
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| Online Access: | https://doi.org/10.1002/cam4.70400 |
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| author | Rabeah Al‐Temaimi Bicher Abdulkarim Ali Al‐Ali Bency John Mrinmay Kumar Mallik Kusum Kapila |
| author_facet | Rabeah Al‐Temaimi Bicher Abdulkarim Ali Al‐Ali Bency John Mrinmay Kumar Mallik Kusum Kapila |
| author_sort | Rabeah Al‐Temaimi |
| collection | DOAJ |
| description | ABSTRACT Background Pancreatic cancer (PC) is one of the most aggressive types of cancer. Despite advances in molecular diagnostics, PC diagnosis relies on imaging technologies and morphological assessment of fine needle aspirates (FNAs). MicroRNA (miRNA) involvement in PC pathogenesis and potential diagnostics application have been suggested, albeit current supporting evidence is lacking. Here, we investigated the association of selected miRNAs with PC incidence and clinical characteristics. Methods Fold expression of miR‐216a‐3p, ‐217‐5p, ‐221‐3p, ‐222‐3p, and miR‐196a‐5p was assessed in 73 PC FNA cell‐block sections and 6 healthy pancreas tissues using Taqman advanced miRNA assays. Potential miRNA targets were ascertained using immunocytochemistry. Results miR‐196a‐5p was upregulated in PC compared to healthy pancreatic tissue (β = −0.05, 95% CI: −0.065 – (−0.035); p < 0.001). miR‐221‐3p and miR‐222‐3p fold expression were strongly correlated (r = 0.897, p < 0.001), whereas miR‐196a‐5p fold expression correlated with that of miR‐221‐3p (r = 0.688, p < 0.001) and miR‐222‐3p (r = 0.489, p < 0.001). Moreover, miR‐196a‐5p fold expression positively correlated with tumor stage (r = 0.32, p = 0.034). miR‐217‐5p fold expression inversely correlated with KRAS expression (r = ‐0.69, p = 0.0027). Conclusion Our study shows miR‐196a‐5p has reasonable specificity to PC and thus may have diagnostic and prognostic potential in PC as proposed in the literature. Moreover, KRAS and NFKBIA may be potential targets for miR‐217‐5p and miR‐196a‐5p, respectively. Thus, these miRNAs may be involved in tumor progression and may have valuable applications in novel therapeutics or treatment monitoring. |
| format | Article |
| id | doaj-art-9f7073969bef46c48e4bc8c1e8958647 |
| institution | Kabale University |
| issn | 2045-7634 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-9f7073969bef46c48e4bc8c1e89586472024-11-13T07:40:35ZengWileyCancer Medicine2045-76342024-11-011321n/an/a10.1002/cam4.70400Analysis of Candidate miRNAs' Expression in Pancreatic CancerRabeah Al‐Temaimi0Bicher Abdulkarim1Ali Al‐Ali2Bency John3Mrinmay Kumar Mallik4Kusum Kapila5Human Genetics Unit, Department of Pathology, College of Medicine Kuwait University Jabriya KuwaitUndergraduate Medical Program, College of Medicine Kuwait University Jabriya KuwaitDepartment of Medicine, College of Medicine Kuwait University Jabriya KuwaitDepartment of Pathology, College of Medicine Kuwait University Jabriya KuwaitDepartment of Laboratory Medicine Mubarak Al Kabeer Hospital Jabriya KuwaitDepartment of Pathology, College of Medicine Kuwait University Jabriya KuwaitABSTRACT Background Pancreatic cancer (PC) is one of the most aggressive types of cancer. Despite advances in molecular diagnostics, PC diagnosis relies on imaging technologies and morphological assessment of fine needle aspirates (FNAs). MicroRNA (miRNA) involvement in PC pathogenesis and potential diagnostics application have been suggested, albeit current supporting evidence is lacking. Here, we investigated the association of selected miRNAs with PC incidence and clinical characteristics. Methods Fold expression of miR‐216a‐3p, ‐217‐5p, ‐221‐3p, ‐222‐3p, and miR‐196a‐5p was assessed in 73 PC FNA cell‐block sections and 6 healthy pancreas tissues using Taqman advanced miRNA assays. Potential miRNA targets were ascertained using immunocytochemistry. Results miR‐196a‐5p was upregulated in PC compared to healthy pancreatic tissue (β = −0.05, 95% CI: −0.065 – (−0.035); p < 0.001). miR‐221‐3p and miR‐222‐3p fold expression were strongly correlated (r = 0.897, p < 0.001), whereas miR‐196a‐5p fold expression correlated with that of miR‐221‐3p (r = 0.688, p < 0.001) and miR‐222‐3p (r = 0.489, p < 0.001). Moreover, miR‐196a‐5p fold expression positively correlated with tumor stage (r = 0.32, p = 0.034). miR‐217‐5p fold expression inversely correlated with KRAS expression (r = ‐0.69, p = 0.0027). Conclusion Our study shows miR‐196a‐5p has reasonable specificity to PC and thus may have diagnostic and prognostic potential in PC as proposed in the literature. Moreover, KRAS and NFKBIA may be potential targets for miR‐217‐5p and miR‐196a‐5p, respectively. Thus, these miRNAs may be involved in tumor progression and may have valuable applications in novel therapeutics or treatment monitoring.https://doi.org/10.1002/cam4.70400KRASMicroRNANFKBIApancreatic cancer |
| spellingShingle | Rabeah Al‐Temaimi Bicher Abdulkarim Ali Al‐Ali Bency John Mrinmay Kumar Mallik Kusum Kapila Analysis of Candidate miRNAs' Expression in Pancreatic Cancer Cancer Medicine KRAS MicroRNA NFKBIA pancreatic cancer |
| title | Analysis of Candidate miRNAs' Expression in Pancreatic Cancer |
| title_full | Analysis of Candidate miRNAs' Expression in Pancreatic Cancer |
| title_fullStr | Analysis of Candidate miRNAs' Expression in Pancreatic Cancer |
| title_full_unstemmed | Analysis of Candidate miRNAs' Expression in Pancreatic Cancer |
| title_short | Analysis of Candidate miRNAs' Expression in Pancreatic Cancer |
| title_sort | analysis of candidate mirnas expression in pancreatic cancer |
| topic | KRAS MicroRNA NFKBIA pancreatic cancer |
| url | https://doi.org/10.1002/cam4.70400 |
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