Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics

Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics

Summary: The silk glands (SGs) of silkworms specifically synthesize silk proteins, thus strongly influencing the yield and quality of silk. Here, we present a protocol for isolating SG nuclei from silkworms and obtaining high-quality tissue slices for spatial transcriptomics. We describe steps for r...

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Bibliographic Details
Main Authors: Yan Ma, Keshu Dong, Jie Hu, Yiyun Tang, Hanfu Xu
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
Subjects:
Cell isolation
Single Cell
Sequencing
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724007469
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