Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics
Summary: The silk glands (SGs) of silkworms specifically synthesize silk proteins, thus strongly influencing the yield and quality of silk. Here, we present a protocol for isolating SG nuclei from silkworms and obtaining high-quality tissue slices for spatial transcriptomics. We describe steps for r...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007469 |
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| author | Yan Ma Keshu Dong Jie Hu Yiyun Tang Hanfu Xu |
| author_facet | Yan Ma Keshu Dong Jie Hu Yiyun Tang Hanfu Xu |
| author_sort | Yan Ma |
| collection | DOAJ |
| description | Summary: The silk glands (SGs) of silkworms specifically synthesize silk proteins, thus strongly influencing the yield and quality of silk. Here, we present a protocol for isolating SG nuclei from silkworms and obtaining high-quality tissue slices for spatial transcriptomics. We describe steps for rearing, dissecting, and nucleus isolation. We then detail procedures for embedding, frozen section, and RNA capturing and sequencing. This protocol enables the exploration of the spatial distribution of SG cells at single-cell resolution.For complete details on the use and execution of this protocol, please refer to Ma et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-9f30d3bf7e2446f680933a861b070f86 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-9f30d3bf7e2446f680933a861b070f862025-01-13T04:19:12ZengElsevierSTAR Protocols2666-16672025-03-0161103581Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomicsYan Ma0Keshu Dong1Jie Hu2Yiyun Tang3Hanfu Xu4State Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China; Corresponding authorState Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, ChinaState Key Laboratory of Resource Insects, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China; Corresponding authorSummary: The silk glands (SGs) of silkworms specifically synthesize silk proteins, thus strongly influencing the yield and quality of silk. Here, we present a protocol for isolating SG nuclei from silkworms and obtaining high-quality tissue slices for spatial transcriptomics. We describe steps for rearing, dissecting, and nucleus isolation. We then detail procedures for embedding, frozen section, and RNA capturing and sequencing. This protocol enables the exploration of the spatial distribution of SG cells at single-cell resolution.For complete details on the use and execution of this protocol, please refer to Ma et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007469Cell isolationSingle CellSequencing |
| spellingShingle | Yan Ma Keshu Dong Jie Hu Yiyun Tang Hanfu Xu Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics STAR Protocols Cell isolation Single Cell Sequencing |
| title | Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics |
| title_full | Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics |
| title_fullStr | Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics |
| title_full_unstemmed | Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics |
| title_short | Protocol for the isolation of silk glands from silkworms for snRNA-seq and spatial transcriptomics |
| title_sort | protocol for the isolation of silk glands from silkworms for snrna seq and spatial transcriptomics |
| topic | Cell isolation Single Cell Sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724007469 |
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