Fabricating rigid nano-grass layer on Ti6Al4V surface by dealloying for enhanced gingival soft tissue integration
Fabricating biofavorable nanostructures on Ti6Al4V abutment surface is a promising strategy for improving gingival soft tissue integration (STI) in order to prevent bacteria invasion. Although some structures show both biocompatible and bacterial repulsive, the decades exposure in oral cavity cannot...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Materials & Design |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0264127524008979 |
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| author | Dongxuan Cai Meiqi Zhao Xi Liu Guangwen Li Yuqi Zhao Haochen Wang Lan Wang Yide He Yumei Zhang Wen Song |
| author_facet | Dongxuan Cai Meiqi Zhao Xi Liu Guangwen Li Yuqi Zhao Haochen Wang Lan Wang Yide He Yumei Zhang Wen Song |
| author_sort | Dongxuan Cai |
| collection | DOAJ |
| description | Fabricating biofavorable nanostructures on Ti6Al4V abutment surface is a promising strategy for improving gingival soft tissue integration (STI) in order to prevent bacteria invasion. Although some structures show both biocompatible and bacterial repulsive, the decades exposure in oral cavity cannot guarantee zero infection. Therefore, developing bioactive and rigid layer is a realistic strategy for clinical scenario. In this study, the dealloying technique was introduced to create nano-grass layer on Ti6Al4V surface by removing Al element. The average size of 30 nm surface could induce the highest and aligned extracellular matrix (ECM) secretion in primary human gingival fibroblasts, accompanied by extensive focal adhesions formation. The epithelial layer in the gingiva-abutment interface was condenser and crosslinked, as indicated by more ECM proteins expression and resistant to HRP invasion. Mechanistically, the cdc42 upregulation and ERK activation account for actin filaments rearrangement and focal adhesions enrichment. Moreover, the grass-like surface showed slight bacteria repulsive property. Thanks to the advantages of dealloying technique, the rigid layer did not show significant damage after conventional scaling by plastic dental scaler. In conclusion, the dealloying technique may be a novel convenient and realistic strategy creating rigid nanoporous surface for both enhancing STI and resistant to physical biofilm removal. |
| format | Article |
| id | doaj-art-9f1693dfff4f4c55ac4a1c28e3096d29 |
| institution | Kabale University |
| issn | 0264-1275 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials & Design |
| spelling | doaj-art-9f1693dfff4f4c55ac4a1c28e3096d292024-12-21T04:27:42ZengElsevierMaterials & Design0264-12752024-12-01248113522Fabricating rigid nano-grass layer on Ti6Al4V surface by dealloying for enhanced gingival soft tissue integrationDongxuan Cai0Meiqi Zhao1Xi Liu2Guangwen Li3Yuqi Zhao4Haochen Wang5Lan Wang6Yide He7Yumei Zhang8Wen Song9State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China; Department of Stomatology, The 908th Hospital of the Chinese People’s Liberation Army Joint Logistic Support Force, Nanchang 330000, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, ChinaShaanxi Key Laboratory of Biomedical Metallic Materials, Northwest Institute for Non-ferrous Metal Research, Xi’an 710016, ChinaState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, National Clinical Research Center for Oral Diseases, Shaanxi Key Laboratory of Stomatology, Department of Operative Dentistry and Endodontics, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi 710032, China; Corresponding authors.State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China; Corresponding authors.State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Key Laboratory of Oral Diseases, Department of Prosthodontics, School of Stomatology, The Fourth Military Medical University, Xi’an 710032, China; Corresponding authors.Fabricating biofavorable nanostructures on Ti6Al4V abutment surface is a promising strategy for improving gingival soft tissue integration (STI) in order to prevent bacteria invasion. Although some structures show both biocompatible and bacterial repulsive, the decades exposure in oral cavity cannot guarantee zero infection. Therefore, developing bioactive and rigid layer is a realistic strategy for clinical scenario. In this study, the dealloying technique was introduced to create nano-grass layer on Ti6Al4V surface by removing Al element. The average size of 30 nm surface could induce the highest and aligned extracellular matrix (ECM) secretion in primary human gingival fibroblasts, accompanied by extensive focal adhesions formation. The epithelial layer in the gingiva-abutment interface was condenser and crosslinked, as indicated by more ECM proteins expression and resistant to HRP invasion. Mechanistically, the cdc42 upregulation and ERK activation account for actin filaments rearrangement and focal adhesions enrichment. Moreover, the grass-like surface showed slight bacteria repulsive property. Thanks to the advantages of dealloying technique, the rigid layer did not show significant damage after conventional scaling by plastic dental scaler. In conclusion, the dealloying technique may be a novel convenient and realistic strategy creating rigid nanoporous surface for both enhancing STI and resistant to physical biofilm removal.http://www.sciencedirect.com/science/article/pii/S0264127524008979Soft tissue integrationTi6Al4V abutmentDealloyingFocal adhesionBacteria |
| spellingShingle | Dongxuan Cai Meiqi Zhao Xi Liu Guangwen Li Yuqi Zhao Haochen Wang Lan Wang Yide He Yumei Zhang Wen Song Fabricating rigid nano-grass layer on Ti6Al4V surface by dealloying for enhanced gingival soft tissue integration Materials & Design Soft tissue integration Ti6Al4V abutment Dealloying Focal adhesion Bacteria |
| title | Fabricating rigid nano-grass layer on Ti6Al4V surface by dealloying for enhanced gingival soft tissue integration |
| title_full | Fabricating rigid nano-grass layer on Ti6Al4V surface by dealloying for enhanced gingival soft tissue integration |
| title_fullStr | Fabricating rigid nano-grass layer on Ti6Al4V surface by dealloying for enhanced gingival soft tissue integration |
| title_full_unstemmed | Fabricating rigid nano-grass layer on Ti6Al4V surface by dealloying for enhanced gingival soft tissue integration |
| title_short | Fabricating rigid nano-grass layer on Ti6Al4V surface by dealloying for enhanced gingival soft tissue integration |
| title_sort | fabricating rigid nano grass layer on ti6al4v surface by dealloying for enhanced gingival soft tissue integration |
| topic | Soft tissue integration Ti6Al4V abutment Dealloying Focal adhesion Bacteria |
| url | http://www.sciencedirect.com/science/article/pii/S0264127524008979 |
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