Experimental analysis of polymorphic microsatellite loci in Cladocera: mixed breeding strategy and population dynamics
The aim of this work is to identify polymorphic loci in Daphnia genomes using genome sequence analysis and experimental analysis of real accessions of samples collected in nature. We use primers predicted by an algorithm for DNA amplification from Daph- nia samples and capillary electrophoresis to...
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Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»
2025-03-01
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| Series: | Письма в Вавиловский журнал генетики и селекции |
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| Online Access: | https://pismavavilov.ru/wp-content/uploads/2025/03/004-Pisma-VJ_Poroshina.pdf |
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| author | Poroshina A.A. Sherbakov D.Yu. |
| author_facet | Poroshina A.A. Sherbakov D.Yu. |
| author_sort | Poroshina A.A. |
| collection | DOAJ |
| description | The aim of this work is to identify polymorphic loci in Daphnia genomes using genome sequence analysis and experimental
analysis of real accessions of samples collected in nature. We use primers predicted by an algorithm for DNA amplification from Daph-
nia samples and capillary electrophoresis to detect polymorphic loci. This was done to detect polymorphic loci in a single experiment,
when the presence of polymorphic loci with a frequency sufficient for genetic studies is detected as the appearance of multiple peaks
on the electrophoregram. Thus, we evaluate the effectiveness of the capillary electrophoresis method and refine population para-
meters, including the proportion of sexual reproduction and the effective population size. Studying polymorphic loci becomes es-
pecially relevant in the context of climate change and anthropogenic impacts on ecosystems, as these factors can significantly affect
genetic diversity and adaptation in populations. Polymorphic loci can serve as indicators of genetic stability and the ability of species
to adapt to changing environmental conditions. In the face of global climate change and increasing anthropogenic impacts, such as
pollution, urbanization, and landscape changes, populations face new challenges that require rapid adaptation. Polymorphic loci, due
to their high variability, can provide valuable information about the genetic structure of populations and their potential for adapta-
tion. In the context of our research, studying polymorphic loci in Daphnia becomes particularly important, given their ecological role
and sensitivity to environmental changes. Daphnia, as key components of freshwater ecosystems, can serve as model organisms for
studying genetic adaptation to climate change and anthropogenic impacts. Understanding polymorphic loci in Daphnia can provide
valuable data for assessing genetic stability and adaptive potential in these populations, which is particularly important in the face of
rapidly changing environmental conditions. |
| format | Article |
| id | doaj-art-9f0f8d0064be4fbb8429b6d5517d149d |
| institution | Kabale University |
| issn | 2686-8482 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Федеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук» |
| record_format | Article |
| series | Письма в Вавиловский журнал генетики и селекции |
| spelling | doaj-art-9f0f8d0064be4fbb8429b6d5517d149d2025-08-20T03:44:21ZengФедеральное государственное бюджетное научное учреждение «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»Письма в Вавиловский журнал генетики и селекции2686-84822025-03-01111121610.18699/letvjgb-2025-11-03Experimental analysis of polymorphic microsatellite loci in Cladocera: mixed breeding strategy and population dynamicsPoroshina A.A.0https://orcid.org/0000-0002-3234-8955 Sherbakov D.Yu.1https://orcid.org/0000-0002-1410-392XLimnological Institute of the Siberian Branch of the Russian Academy of Sciences, Irkutsk, RussiaLimnological Institute of the Siberian Branch of the Russian Academy of Sciences, Irkutsk, RussiaThe aim of this work is to identify polymorphic loci in Daphnia genomes using genome sequence analysis and experimental analysis of real accessions of samples collected in nature. We use primers predicted by an algorithm for DNA amplification from Daph- nia samples and capillary electrophoresis to detect polymorphic loci. This was done to detect polymorphic loci in a single experiment, when the presence of polymorphic loci with a frequency sufficient for genetic studies is detected as the appearance of multiple peaks on the electrophoregram. Thus, we evaluate the effectiveness of the capillary electrophoresis method and refine population para- meters, including the proportion of sexual reproduction and the effective population size. Studying polymorphic loci becomes es- pecially relevant in the context of climate change and anthropogenic impacts on ecosystems, as these factors can significantly affect genetic diversity and adaptation in populations. Polymorphic loci can serve as indicators of genetic stability and the ability of species to adapt to changing environmental conditions. In the face of global climate change and increasing anthropogenic impacts, such as pollution, urbanization, and landscape changes, populations face new challenges that require rapid adaptation. Polymorphic loci, due to their high variability, can provide valuable information about the genetic structure of populations and their potential for adapta- tion. In the context of our research, studying polymorphic loci in Daphnia becomes particularly important, given their ecological role and sensitivity to environmental changes. Daphnia, as key components of freshwater ecosystems, can serve as model organisms for studying genetic adaptation to climate change and anthropogenic impacts. Understanding polymorphic loci in Daphnia can provide valuable data for assessing genetic stability and adaptive potential in these populations, which is particularly important in the face of rapidly changing environmental conditions.https://pismavavilov.ru/wp-content/uploads/2025/03/004-Pisma-VJ_Poroshina.pdfpolymorphic loci;capillary electrophoresisagent-based modelingmicrosatellitesdaphniagenetic diversityadaptation;climate changeanthropogenic impacts |
| spellingShingle | Poroshina A.A. Sherbakov D.Yu. Experimental analysis of polymorphic microsatellite loci in Cladocera: mixed breeding strategy and population dynamics Письма в Вавиловский журнал генетики и селекции polymorphic loci; capillary electrophoresis agent-based modeling microsatellites daphnia genetic diversity adaptation; climate change anthropogenic impacts |
| title | Experimental analysis of polymorphic microsatellite loci in Cladocera: mixed breeding strategy and population dynamics |
| title_full | Experimental analysis of polymorphic microsatellite loci in Cladocera: mixed breeding strategy and population dynamics |
| title_fullStr | Experimental analysis of polymorphic microsatellite loci in Cladocera: mixed breeding strategy and population dynamics |
| title_full_unstemmed | Experimental analysis of polymorphic microsatellite loci in Cladocera: mixed breeding strategy and population dynamics |
| title_short | Experimental analysis of polymorphic microsatellite loci in Cladocera: mixed breeding strategy and population dynamics |
| title_sort | experimental analysis of polymorphic microsatellite loci in cladocera mixed breeding strategy and population dynamics |
| topic | polymorphic loci; capillary electrophoresis agent-based modeling microsatellites daphnia genetic diversity adaptation; climate change anthropogenic impacts |
| url | https://pismavavilov.ru/wp-content/uploads/2025/03/004-Pisma-VJ_Poroshina.pdf |
| work_keys_str_mv | AT poroshinaaa experimentalanalysisofpolymorphicmicrosatellitelociincladoceramixedbreedingstrategyandpopulationdynamics AT sherbakovdyu experimentalanalysisofpolymorphicmicrosatellitelociincladoceramixedbreedingstrategyandpopulationdynamics |