Biosynthesis of a dual growth factors (GFs) functionalized silk sericin hydrogel to promote chronic wound healing in diabetic mice
Chronic non-healing wounds, such as diabetic foot, pressure sores and bedsores have seriously affected the life quality of patients worldwide. GFs provide a potential solution to promote chronic wound healing by promoting cell proliferation and differentiation. However, limited resource, high cost,...
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KeAi Communications Co., Ltd.
2025-10-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X25002452 |
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| author | Hanxin Deng Fangyu Wang Yujuan Zhou Hexu Lei Hongji Zhou Siyu Chen Zihan Meng Mengyao He Ding Tu He Wang Xian Li Qingyou Xia Xueming Li Feng Wang |
| author_facet | Hanxin Deng Fangyu Wang Yujuan Zhou Hexu Lei Hongji Zhou Siyu Chen Zihan Meng Mengyao He Ding Tu He Wang Xian Li Qingyou Xia Xueming Li Feng Wang |
| author_sort | Hanxin Deng |
| collection | DOAJ |
| description | Chronic non-healing wounds, such as diabetic foot, pressure sores and bedsores have seriously affected the life quality of patients worldwide. GFs provide a potential solution to promote chronic wound healing by promoting cell proliferation and differentiation. However, limited resource, high cost, and instability in vivo greatly hindered their clinical applications. In present study, two silk gland bioreactor silkworm stains were generated to successfully synthesize functional silk fibers incorporating high expressions of EGF and PDGF-BB. The two GFs functionalized silk raw materials were used to fabricate a dual GFs sericin hydrogel (E/P-SH) delivering system with tunable material performances for better cell adhesion and biocompatibility, sustainable release of the dual GFs to synergistically promote cell proliferation and migration, which realized the significant healing of chronic full-thickness skin wound in diabetic mice within 12 days with more organized collagen arrangement and better epithelialization degree by reducing inflammatory response and promoting vascularization. These findings demonstrated that the biosynthesized dual GFs-SH delivering system provides an opportunity to broaden the wide utility of GFs in clinical treatment of diabetic wound healing. |
| format | Article |
| id | doaj-art-9ee3935a7bb34a5db7e9d85a33ac93b5 |
| institution | Kabale University |
| issn | 2452-199X |
| language | English |
| publishDate | 2025-10-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj-art-9ee3935a7bb34a5db7e9d85a33ac93b52025-08-24T05:13:45ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-10-015251152810.1016/j.bioactmat.2025.06.017Biosynthesis of a dual growth factors (GFs) functionalized silk sericin hydrogel to promote chronic wound healing in diabetic miceHanxin Deng0Fangyu Wang1Yujuan Zhou2Hexu Lei3Hongji Zhou4Siyu Chen5Zihan Meng6Mengyao He7Ding Tu8He Wang9Xian Li10Qingyou Xia11Xueming Li12Feng Wang13Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, ChinaIntegrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, ChinaKey Laboratory of Birth Defects and Related Diseases of Women and Children of Ministry of Education, Department of Radiology, West China Second University Hospital, Sichuan University, 20# South Renmin Road, Chengdu, Sichuan, China; Corresponding author.Integrative Science Center of Germplasm Creation in Western China (CHONGQING) Science City, Biological Science Research Center, Southwest University, Chongqing, 400715, China; Corresponding author.Chronic non-healing wounds, such as diabetic foot, pressure sores and bedsores have seriously affected the life quality of patients worldwide. GFs provide a potential solution to promote chronic wound healing by promoting cell proliferation and differentiation. However, limited resource, high cost, and instability in vivo greatly hindered their clinical applications. In present study, two silk gland bioreactor silkworm stains were generated to successfully synthesize functional silk fibers incorporating high expressions of EGF and PDGF-BB. The two GFs functionalized silk raw materials were used to fabricate a dual GFs sericin hydrogel (E/P-SH) delivering system with tunable material performances for better cell adhesion and biocompatibility, sustainable release of the dual GFs to synergistically promote cell proliferation and migration, which realized the significant healing of chronic full-thickness skin wound in diabetic mice within 12 days with more organized collagen arrangement and better epithelialization degree by reducing inflammatory response and promoting vascularization. These findings demonstrated that the biosynthesized dual GFs-SH delivering system provides an opportunity to broaden the wide utility of GFs in clinical treatment of diabetic wound healing.http://www.sciencedirect.com/science/article/pii/S2452199X25002452BiosynthesisSericin hydrogelsGrowth factorsCell proliferationChronic diabetic wound healing |
| spellingShingle | Hanxin Deng Fangyu Wang Yujuan Zhou Hexu Lei Hongji Zhou Siyu Chen Zihan Meng Mengyao He Ding Tu He Wang Xian Li Qingyou Xia Xueming Li Feng Wang Biosynthesis of a dual growth factors (GFs) functionalized silk sericin hydrogel to promote chronic wound healing in diabetic mice Bioactive Materials Biosynthesis Sericin hydrogels Growth factors Cell proliferation Chronic diabetic wound healing |
| title | Biosynthesis of a dual growth factors (GFs) functionalized silk sericin hydrogel to promote chronic wound healing in diabetic mice |
| title_full | Biosynthesis of a dual growth factors (GFs) functionalized silk sericin hydrogel to promote chronic wound healing in diabetic mice |
| title_fullStr | Biosynthesis of a dual growth factors (GFs) functionalized silk sericin hydrogel to promote chronic wound healing in diabetic mice |
| title_full_unstemmed | Biosynthesis of a dual growth factors (GFs) functionalized silk sericin hydrogel to promote chronic wound healing in diabetic mice |
| title_short | Biosynthesis of a dual growth factors (GFs) functionalized silk sericin hydrogel to promote chronic wound healing in diabetic mice |
| title_sort | biosynthesis of a dual growth factors gfs functionalized silk sericin hydrogel to promote chronic wound healing in diabetic mice |
| topic | Biosynthesis Sericin hydrogels Growth factors Cell proliferation Chronic diabetic wound healing |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X25002452 |
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