Myelin-associated proteins are potential diagnostic markers in patients with primary brain tumour
Introduction Taking into account the possibility of myelin-associated proteins having a role in brain tumour development, the study aimed to evaluate the diagnostic usefulness of myelin-associated proteins (Nogo-A, MAG, OMgp) released into extracellular space in patients with brain tumours.Patients...
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Taylor & Francis Group
2021-01-01
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| Series: | Annals of Medicine |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/07853890.2021.1983205 |
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| author | Olga M. Koper-Lenkiewicz Anna J. Milewska Joanna Kamińska Karol Sawicki Robert Chrzanowski Justyna Zińczuk Joanna Reszeć Marzena Tylicka Ewa Matuszczak Joanna Matowicka-Karna Zenon Mariak Mariusz W. Mucha Robert Pawlak Violetta Dymicka-Piekarska |
| author_facet | Olga M. Koper-Lenkiewicz Anna J. Milewska Joanna Kamińska Karol Sawicki Robert Chrzanowski Justyna Zińczuk Joanna Reszeć Marzena Tylicka Ewa Matuszczak Joanna Matowicka-Karna Zenon Mariak Mariusz W. Mucha Robert Pawlak Violetta Dymicka-Piekarska |
| author_sort | Olga M. Koper-Lenkiewicz |
| collection | DOAJ |
| description | Introduction Taking into account the possibility of myelin-associated proteins having a role in brain tumour development, the study aimed to evaluate the diagnostic usefulness of myelin-associated proteins (Nogo-A, MAG, OMgp) released into extracellular space in patients with brain tumours.Patients and methods Protein concentration in primary brain tumour (n = 49) and non-tumoural subjects (n = 24) was measured in cerebrospinal fluid (CSF) and serum by means of ELISA. Immunohistochemistry for IDH1-R132H was done on 5-μm thick formalin-fixed, paraffin-embedded tumour sections with the use of an antibody specific for the mutant IDH1-R132H protein.Results The receiver operator characteristic curve analysis showed that CSF Nogo-A and serum MAG were useful in differentiating patients with primary brain tumour from non-tumoural individuals. This was also true in the case of the separate analysis of the astrocytic tumour versus non-tumoural groups and the meningeal tumour versus non-tumoural groups. Neither Nogo-A nor MAG or OMgp concentrations were significantly different, in serum or CSF, between IDH1 wild-type astrocytic brain tumour patients compared to IDH1 mutant patients.Conclusions Our results indicated the potential usefulness of CSF Nogo-A and serum MAG evaluation as circulating biomarkers of primary brain tumours. Because blood is relatively easy to obtain, future research should be conducted to explicitly indicate the value of serum MAG concentration evaluation as a brain tumour biomarker.Key messagesMyelin-associated proteins may be circulating brain tumour biomarkers.Nogo-A and MAG proteins seem to be the most useful in brain tumour diagnosis.Decreased CSF Nogo-A concentration is an adverse prognostic factor for patients’ survival. |
| format | Article |
| id | doaj-art-9ed92d9a21d8480c91a0817fdd8d9ed3 |
| institution | Kabale University |
| issn | 0785-3890 1365-2060 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Annals of Medicine |
| spelling | doaj-art-9ed92d9a21d8480c91a0817fdd8d9ed32025-08-20T03:44:04ZengTaylor & Francis GroupAnnals of Medicine0785-38901365-20602021-01-015311710172110.1080/07853890.2021.1983205Myelin-associated proteins are potential diagnostic markers in patients with primary brain tumourOlga M. Koper-Lenkiewicz0Anna J. Milewska1Joanna Kamińska2Karol Sawicki3Robert Chrzanowski4Justyna Zińczuk5Joanna Reszeć6Marzena Tylicka7Ewa Matuszczak8Joanna Matowicka-Karna9Zenon Mariak10Mariusz W. Mucha11Robert Pawlak12Violetta Dymicka-Piekarska13Department of Clinical Laboratory Diagnostics, Medical University of Bialystok, Białystok, PolandDepartment of Statistics and Medical Informatics, Medical University of Bialystok, Białystok, PolandDepartment of Clinical Laboratory Diagnostics, Medical University of Bialystok, Białystok, PolandDepartment of Neurosurgery, Medical University of Bialystok, Białystok, PolandDepartment of Neurosurgery, Medical University of Bialystok, Białystok, PolandDepartment of Clinical Laboratory Diagnostics, Medical University of Bialystok, Białystok, PolandDepartment of Medical Pathomorphology, Medical University of Bialystok, Białystok, PolandDepartment of Biophysics, Medical University of Białystok, Białystok, PolandDepartment of Pediatric Surgery, Medical University of Białystok, Białystok, PolandDepartment of Clinical Laboratory Diagnostics, Medical University of Bialystok, Białystok, PolandDepartment of Neurosurgery, Medical University of Bialystok, Białystok, PolandInstitute of Biomedical and Clinical Science, Hatherly Laboratories, University of Exeter Medical School, Exeter, UKInstitute of Biomedical and Clinical Science, Hatherly Laboratories, University of Exeter Medical School, Exeter, UKDepartment of Clinical Laboratory Diagnostics, Medical University of Bialystok, Białystok, PolandIntroduction Taking into account the possibility of myelin-associated proteins having a role in brain tumour development, the study aimed to evaluate the diagnostic usefulness of myelin-associated proteins (Nogo-A, MAG, OMgp) released into extracellular space in patients with brain tumours.Patients and methods Protein concentration in primary brain tumour (n = 49) and non-tumoural subjects (n = 24) was measured in cerebrospinal fluid (CSF) and serum by means of ELISA. Immunohistochemistry for IDH1-R132H was done on 5-μm thick formalin-fixed, paraffin-embedded tumour sections with the use of an antibody specific for the mutant IDH1-R132H protein.Results The receiver operator characteristic curve analysis showed that CSF Nogo-A and serum MAG were useful in differentiating patients with primary brain tumour from non-tumoural individuals. This was also true in the case of the separate analysis of the astrocytic tumour versus non-tumoural groups and the meningeal tumour versus non-tumoural groups. Neither Nogo-A nor MAG or OMgp concentrations were significantly different, in serum or CSF, between IDH1 wild-type astrocytic brain tumour patients compared to IDH1 mutant patients.Conclusions Our results indicated the potential usefulness of CSF Nogo-A and serum MAG evaluation as circulating biomarkers of primary brain tumours. Because blood is relatively easy to obtain, future research should be conducted to explicitly indicate the value of serum MAG concentration evaluation as a brain tumour biomarker.Key messagesMyelin-associated proteins may be circulating brain tumour biomarkers.Nogo-A and MAG proteins seem to be the most useful in brain tumour diagnosis.Decreased CSF Nogo-A concentration is an adverse prognostic factor for patients’ survival.https://www.tandfonline.com/doi/10.1080/07853890.2021.1983205Biomarkercerebrospinal fluidmyelin-associated proteinsprimary brain tumour |
| spellingShingle | Olga M. Koper-Lenkiewicz Anna J. Milewska Joanna Kamińska Karol Sawicki Robert Chrzanowski Justyna Zińczuk Joanna Reszeć Marzena Tylicka Ewa Matuszczak Joanna Matowicka-Karna Zenon Mariak Mariusz W. Mucha Robert Pawlak Violetta Dymicka-Piekarska Myelin-associated proteins are potential diagnostic markers in patients with primary brain tumour Annals of Medicine Biomarker cerebrospinal fluid myelin-associated proteins primary brain tumour |
| title | Myelin-associated proteins are potential diagnostic markers in patients with primary brain tumour |
| title_full | Myelin-associated proteins are potential diagnostic markers in patients with primary brain tumour |
| title_fullStr | Myelin-associated proteins are potential diagnostic markers in patients with primary brain tumour |
| title_full_unstemmed | Myelin-associated proteins are potential diagnostic markers in patients with primary brain tumour |
| title_short | Myelin-associated proteins are potential diagnostic markers in patients with primary brain tumour |
| title_sort | myelin associated proteins are potential diagnostic markers in patients with primary brain tumour |
| topic | Biomarker cerebrospinal fluid myelin-associated proteins primary brain tumour |
| url | https://www.tandfonline.com/doi/10.1080/07853890.2021.1983205 |
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