Impact of foliar application of phyllosphere yeast strains combined with soil fertilizer application on rice growth and yield

Abstract Background The application of beneficial microbes in agriculture is gaining increasing attention as a means to reduce reliance on chemical fertilizers. This approach can potentially mitigate negative impacts on soil, animal, and human health, as well as decrease climate-changing factors. Am...

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Main Authors: Gomathy Muthukrishanan, Jeyashri Munisamy, Sabarinathan Kuttalingam Gopalasubramaniam, Kizaharael Sevathapandian Subramanian, Rajakumar Dharmaraj, Dhruba Jyoti Nath, Pranab Dutta, Arun Kumar Devarajan
Format: Article
Language:English
Published: BMC 2024-12-01
Series:Environmental Microbiome
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Online Access:https://doi.org/10.1186/s40793-024-00635-9
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author Gomathy Muthukrishanan
Jeyashri Munisamy
Sabarinathan Kuttalingam Gopalasubramaniam
Kizaharael Sevathapandian Subramanian
Rajakumar Dharmaraj
Dhruba Jyoti Nath
Pranab Dutta
Arun Kumar Devarajan
author_facet Gomathy Muthukrishanan
Jeyashri Munisamy
Sabarinathan Kuttalingam Gopalasubramaniam
Kizaharael Sevathapandian Subramanian
Rajakumar Dharmaraj
Dhruba Jyoti Nath
Pranab Dutta
Arun Kumar Devarajan
author_sort Gomathy Muthukrishanan
collection DOAJ
description Abstract Background The application of beneficial microbes in agriculture is gaining increasing attention as a means to reduce reliance on chemical fertilizers. This approach can potentially mitigate negative impacts on soil, animal, and human health, as well as decrease climate-changing factors. Among these microbes, yeast has been the least explored, particularly within the phyllosphere compartment. This study addresses this knowledge gap by investigating the potential of phyllosphere yeast to improve rice yield while reducing fertilizer dosage. Results From fifty-two rice yeast phyllosphere isolates, we identified three yeast strains—Rhodotorula paludigena Y1, Pseudozyma sp. Y71, and Cryptococcus sp. Y72—that could thrive at 36 °C and possessed significant multifarious plant growth-promoting traits, enhancing rice root and shoot length upon seed inoculation. These three strains demonstrated favorable compatibility, leading to the creation of a yeast consortium. We assessed the combined effect of foliar application of this yeast consortium and individual strains with two distinct recommended doses of chemical fertilizers (RDCFs) (75 and 100%), as well as RDCFs alone (75 and 100%), in rice maintained in pot-culture and field experiments. The pot-culture experiment investigated the leaf microbial community, plant biochemicals, root and shoot length during the stem elongation, flowering, and dough phases, and yield-related parameters at harvest. The field experiment determined the actual yield. Integrated results from both experiments revealed that the yeast consortium with 75% RDCFs was more effective than the yeast consortium with 100% RDCFs, single strain applications with RDCFs (75 and 100%), and RDCFs alone (75 and 100%). Additionally, this treatment improved leaf metabolite levels compared to control rice plants. Conclusions Overall, a 25% reduction in soil chemical fertilizers combined with yeast consortium foliar application improved rice growth, biochemicals, and yield. This study also advances the field of phyllosphere yeast research in agriculture.
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spelling doaj-art-9ec407fa7dbb4e2aba8913fad8a3d01a2024-12-22T12:48:44ZengBMCEnvironmental Microbiome2524-63722024-12-0119112410.1186/s40793-024-00635-9Impact of foliar application of phyllosphere yeast strains combined with soil fertilizer application on rice growth and yieldGomathy Muthukrishanan0Jeyashri Munisamy1Sabarinathan Kuttalingam Gopalasubramaniam2Kizaharael Sevathapandian Subramanian3Rajakumar Dharmaraj4Dhruba Jyoti Nath5Pranab Dutta6Arun Kumar Devarajan7Department of Soil Science and Agricultural Chemistry, Agricultural College and Research Institute, Tamil Nadu Agricultural UniversityDepartment of Soil Science and Agricultural Chemistry, Agricultural College and Research Institute, Tamil Nadu Agricultural UniversityDepartment of Plant Pathology, Agricultural College and Research Institute, Tamil Nadu Agricultural UniversityDepartment of Nanotechnology, Tamil Nadu Agricultural UniversityAgricultural College and Research InstituteAssam Agricultural UniversityCentral Agricultural UniversityTamil Nadu Agricultural UniversityAbstract Background The application of beneficial microbes in agriculture is gaining increasing attention as a means to reduce reliance on chemical fertilizers. This approach can potentially mitigate negative impacts on soil, animal, and human health, as well as decrease climate-changing factors. Among these microbes, yeast has been the least explored, particularly within the phyllosphere compartment. This study addresses this knowledge gap by investigating the potential of phyllosphere yeast to improve rice yield while reducing fertilizer dosage. Results From fifty-two rice yeast phyllosphere isolates, we identified three yeast strains—Rhodotorula paludigena Y1, Pseudozyma sp. Y71, and Cryptococcus sp. Y72—that could thrive at 36 °C and possessed significant multifarious plant growth-promoting traits, enhancing rice root and shoot length upon seed inoculation. These three strains demonstrated favorable compatibility, leading to the creation of a yeast consortium. We assessed the combined effect of foliar application of this yeast consortium and individual strains with two distinct recommended doses of chemical fertilizers (RDCFs) (75 and 100%), as well as RDCFs alone (75 and 100%), in rice maintained in pot-culture and field experiments. The pot-culture experiment investigated the leaf microbial community, plant biochemicals, root and shoot length during the stem elongation, flowering, and dough phases, and yield-related parameters at harvest. The field experiment determined the actual yield. Integrated results from both experiments revealed that the yeast consortium with 75% RDCFs was more effective than the yeast consortium with 100% RDCFs, single strain applications with RDCFs (75 and 100%), and RDCFs alone (75 and 100%). Additionally, this treatment improved leaf metabolite levels compared to control rice plants. Conclusions Overall, a 25% reduction in soil chemical fertilizers combined with yeast consortium foliar application improved rice growth, biochemicals, and yield. This study also advances the field of phyllosphere yeast research in agriculture.https://doi.org/10.1186/s40793-024-00635-9YeastPhyllospherePlant growth-promoting microbesRiceCrop improvement
spellingShingle Gomathy Muthukrishanan
Jeyashri Munisamy
Sabarinathan Kuttalingam Gopalasubramaniam
Kizaharael Sevathapandian Subramanian
Rajakumar Dharmaraj
Dhruba Jyoti Nath
Pranab Dutta
Arun Kumar Devarajan
Impact of foliar application of phyllosphere yeast strains combined with soil fertilizer application on rice growth and yield
Environmental Microbiome
Yeast
Phyllosphere
Plant growth-promoting microbes
Rice
Crop improvement
title Impact of foliar application of phyllosphere yeast strains combined with soil fertilizer application on rice growth and yield
title_full Impact of foliar application of phyllosphere yeast strains combined with soil fertilizer application on rice growth and yield
title_fullStr Impact of foliar application of phyllosphere yeast strains combined with soil fertilizer application on rice growth and yield
title_full_unstemmed Impact of foliar application of phyllosphere yeast strains combined with soil fertilizer application on rice growth and yield
title_short Impact of foliar application of phyllosphere yeast strains combined with soil fertilizer application on rice growth and yield
title_sort impact of foliar application of phyllosphere yeast strains combined with soil fertilizer application on rice growth and yield
topic Yeast
Phyllosphere
Plant growth-promoting microbes
Rice
Crop improvement
url https://doi.org/10.1186/s40793-024-00635-9
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