Black Goji Berry Extract as a Natural Photosensitizer for Photodynamic Inactivation of Microbial Strains: A Promising Approach
Introduction: Antimicrobial resistance is a critical public health concern directly contributing to many deaths globally. Unearthing new protocols to tackle pathogenic microorganisms is vital. Photodynamic inactivation (PDI) is an efficient method for treating cancer and chronic microbial infecti...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2023-12-01
|
| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Introduction: Antimicrobial resistance is a critical public health concern
directly contributing to many deaths globally. Unearthing new protocols to
tackle pathogenic microorganisms is vital. Photodynamic inactivation (PDI)
is an efficient method for treating cancer and chronic microbial infections.
PDI merges light with a photosensitizer to prompt the generation of reactive
oxygen species and free radicals, not including O2. These molecules cause
a phototoxic reaction and lead to the death of microorganisms. Recent
advancements in photodynamic inactivation are focused on the exploration
of natural photosensitizers. Methods: In this study, Black Goji Berry
Extract (BGBE) was utilized as a photosensitizer at 50 mg/mL
concentration. The incubation periods for the extract were 10 and 15
minutes, respectively. Phototoxic reactions were induced using ultraviolet
(UV) light, specifically within the 315-400 nm range at an intensity of 8W,
for an illumination period of 5 min. This study utilized microbial strains of
Staphylococcus aureus, Candida albicans, Pseudomonas aeruginosa, and
Escherichia coli as test subjects. All the strains went through treatments
under these specified conditions. Results were reported after comparing
controls and treatments. Results: Incubating with BGBE for 10-15 min
followed by a 5-min black UV illumination induced a phototoxic reaction
in bacterial strains such as S. aureus, P. aeruginosa, and E. coli. This
resulted in a decrease in bacterial viability by 0.75-0.92 log10 (CFU/mL). No
significant difference was observed between the effects on Gram-positive
and Gram-negative strains (P>0.05). Moreover, no phototoxic reaction was
observed in Candida albicans under similar conditions. Conclusion: While
a sublethal photodynamic reaction was recorded in specific bacterial strains
post-photodynamic inactivation utilizing BGBE, BGB exhibits potential as
a natural source for innovative photosensitizers. |
|---|---|
| ISSN: | 2345-5349 2345-5330 |