Overexpression of <i>AtruLEA1</i> from <i>Acer truncatum</i> Bunge Enhanced <i>Arabidopsis</i> Drought and Salt Tolerance by Improving ROS-Scavenging Capability
<i>Late embryonic developmental abundant</i> (<i>LEA</i>) genes play a crucial role in the response to abiotic stress and are important target genes for research on plant stress tolerance mechanisms. <i>Acer truncatum</i> Bunge is a promising candidate tree specie...
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2025-01-01
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| author | Shaofeng Li Huijing Meng Yanfei Yang Jinna Zhao Yongxiu Xia Shaoli Wang Fei Wang Guangshun Zheng Jianbo Li |
| author_facet | Shaofeng Li Huijing Meng Yanfei Yang Jinna Zhao Yongxiu Xia Shaoli Wang Fei Wang Guangshun Zheng Jianbo Li |
| author_sort | Shaofeng Li |
| collection | DOAJ |
| description | <i>Late embryonic developmental abundant</i> (<i>LEA</i>) genes play a crucial role in the response to abiotic stress and are important target genes for research on plant stress tolerance mechanisms. <i>Acer truncatum</i> Bunge is a promising candidate tree species for investigating the tolerance mechanism of woody plants against abiotic stress. In our previous study, <i>AtruLEA1</i> was identified as being associated with seed drought tolerance. In this study, <i>LEA1</i> was cloned from <i>A. truncatum</i> Bunge and functionally characterized. <i>AtruLEA1</i> encodes an LEA protein and is located in the nucleus. Phylogenetic tree analysis revealed a recent affinity of the AtruLEA1 protein to AT3G15760.1. Overexpression of <i>AtruLEA1</i> resulted in enhanced tolerance of <i>Arabidopsis thaliana</i> to drought and salt stress and heightened the ABA sensitivity. Compared to wild-type (WT) plants, plants with overexpressed <i>AtruLEA1</i> exhibited increased activities of antioxidant enzymes under drought stress. Meanwhile, the ROS level of transgenic <i>Arabidopsis</i> was significantly less than that of the WT. Additionally, the stoma density and stoma openness of <i>AtruLEA1 Arabidopsis</i> were higher compared to those in the WT <i>Arabidopsis</i> under salt and drought stress conditions, which ensures that the biomass and relative water content of transgenic <i>Arabidopsis</i> are significantly better than those of the WT. These results indicated that <i>AtruLEA1</i> was involved in salt and drought stress tolerances by maintaining ROS homeostasis, and its expression was positively regulated by abiotic stress. These results indicate a positive role of AtruLEA1 in drought and salt stress and provide theoretical evidence in the direction of cultivating resistant plants. |
| format | Article |
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| institution | Kabale University |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj-art-9ea6f0fc88b44c04b1ea779c03967b5b2025-01-10T13:19:48ZengMDPI AGPlants2223-77472025-01-0114111710.3390/plants14010117Overexpression of <i>AtruLEA1</i> from <i>Acer truncatum</i> Bunge Enhanced <i>Arabidopsis</i> Drought and Salt Tolerance by Improving ROS-Scavenging CapabilityShaofeng Li0Huijing Meng1Yanfei Yang2Jinna Zhao3Yongxiu Xia4Shaoli Wang5Fei Wang6Guangshun Zheng7Jianbo Li8State Key Laboratory of Tree Genetics and Breeding, Experimental Center of Forestry in North China, National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in Beijing, Chinese Academy of Forestry, Beijing 100091, ChinaCollege of Forestry, Henan Agricultural University, Zhengzhou 450046, ChinaCollege of Forestry, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Forestry, Shanxi Agricultural University, Taigu 030801, ChinaState Key Laboratory of Tree Genetics and Breeding, Experimental Center of Forestry in North China, National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in Beijing, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Experimental Center of Forestry in North China, National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in Beijing, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Experimental Center of Forestry in North China, National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in Beijing, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Experimental Center of Forestry in North China, National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in Beijing, Chinese Academy of Forestry, Beijing 100091, ChinaState Key Laboratory of Tree Genetics and Breeding, Experimental Center of Forestry in North China, National Permanent Scientific Research Base for Warm Temperate Zone Forestry of Jiulong Mountain in Beijing, Chinese Academy of Forestry, Beijing 100091, China<i>Late embryonic developmental abundant</i> (<i>LEA</i>) genes play a crucial role in the response to abiotic stress and are important target genes for research on plant stress tolerance mechanisms. <i>Acer truncatum</i> Bunge is a promising candidate tree species for investigating the tolerance mechanism of woody plants against abiotic stress. In our previous study, <i>AtruLEA1</i> was identified as being associated with seed drought tolerance. In this study, <i>LEA1</i> was cloned from <i>A. truncatum</i> Bunge and functionally characterized. <i>AtruLEA1</i> encodes an LEA protein and is located in the nucleus. Phylogenetic tree analysis revealed a recent affinity of the AtruLEA1 protein to AT3G15760.1. Overexpression of <i>AtruLEA1</i> resulted in enhanced tolerance of <i>Arabidopsis thaliana</i> to drought and salt stress and heightened the ABA sensitivity. Compared to wild-type (WT) plants, plants with overexpressed <i>AtruLEA1</i> exhibited increased activities of antioxidant enzymes under drought stress. Meanwhile, the ROS level of transgenic <i>Arabidopsis</i> was significantly less than that of the WT. Additionally, the stoma density and stoma openness of <i>AtruLEA1 Arabidopsis</i> were higher compared to those in the WT <i>Arabidopsis</i> under salt and drought stress conditions, which ensures that the biomass and relative water content of transgenic <i>Arabidopsis</i> are significantly better than those of the WT. These results indicated that <i>AtruLEA1</i> was involved in salt and drought stress tolerances by maintaining ROS homeostasis, and its expression was positively regulated by abiotic stress. These results indicate a positive role of AtruLEA1 in drought and salt stress and provide theoretical evidence in the direction of cultivating resistant plants.https://www.mdpi.com/2223-7747/14/1/117<i>Acer truncatum</i> Bunge<i>LEA1</i>drought stresssalt stressABA |
| spellingShingle | Shaofeng Li Huijing Meng Yanfei Yang Jinna Zhao Yongxiu Xia Shaoli Wang Fei Wang Guangshun Zheng Jianbo Li Overexpression of <i>AtruLEA1</i> from <i>Acer truncatum</i> Bunge Enhanced <i>Arabidopsis</i> Drought and Salt Tolerance by Improving ROS-Scavenging Capability Plants <i>Acer truncatum</i> Bunge <i>LEA1</i> drought stress salt stress ABA |
| title | Overexpression of <i>AtruLEA1</i> from <i>Acer truncatum</i> Bunge Enhanced <i>Arabidopsis</i> Drought and Salt Tolerance by Improving ROS-Scavenging Capability |
| title_full | Overexpression of <i>AtruLEA1</i> from <i>Acer truncatum</i> Bunge Enhanced <i>Arabidopsis</i> Drought and Salt Tolerance by Improving ROS-Scavenging Capability |
| title_fullStr | Overexpression of <i>AtruLEA1</i> from <i>Acer truncatum</i> Bunge Enhanced <i>Arabidopsis</i> Drought and Salt Tolerance by Improving ROS-Scavenging Capability |
| title_full_unstemmed | Overexpression of <i>AtruLEA1</i> from <i>Acer truncatum</i> Bunge Enhanced <i>Arabidopsis</i> Drought and Salt Tolerance by Improving ROS-Scavenging Capability |
| title_short | Overexpression of <i>AtruLEA1</i> from <i>Acer truncatum</i> Bunge Enhanced <i>Arabidopsis</i> Drought and Salt Tolerance by Improving ROS-Scavenging Capability |
| title_sort | overexpression of i atrulea1 i from i acer truncatum i bunge enhanced i arabidopsis i drought and salt tolerance by improving ros scavenging capability |
| topic | <i>Acer truncatum</i> Bunge <i>LEA1</i> drought stress salt stress ABA |
| url | https://www.mdpi.com/2223-7747/14/1/117 |
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