Impact of rhizobacterial inoculation in plant growth medium to mitigate lead stress in Tomato (Solanum lycopersicum L.)
Abstract This study investigates the effects of lead stress on tomato plants and explores the potential role of plant growth-promoting rhizobacteria (PGPR) to alleviate this stress. The experiment was conducted in pots, introducing varying lead levels (0, 100, 200, 300, 400, and 500 mg kg⁻¹) using l...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-84648-x |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559611740520448 |
|---|---|
| author | Sumreen Anjum Mubeen Sarwar Adnan Mukhtar Tanveer Hussain Mehwish Liaquat Alia Batool Muhammad Waqar Alam Qurban Ali M. Irfan Ashraf |
| author_facet | Sumreen Anjum Mubeen Sarwar Adnan Mukhtar Tanveer Hussain Mehwish Liaquat Alia Batool Muhammad Waqar Alam Qurban Ali M. Irfan Ashraf |
| author_sort | Sumreen Anjum |
| collection | DOAJ |
| description | Abstract This study investigates the effects of lead stress on tomato plants and explores the potential role of plant growth-promoting rhizobacteria (PGPR) to alleviate this stress. The experiment was conducted in pots, introducing varying lead levels (0, 100, 200, 300, 400, and 500 mg kg⁻¹) using lead nitrate. For rhizobacterial inoculation, pre-characterized LTPGP strains S5 Pseudomonas fluorescens A506 and S10 Pseudomonas fluorescens LMG 2189 were used. Data were recorded on growth parameters (shoot and root length, fresh and dry weight), physiological attributes (chlorophyll content, proline content, electrolyte leakage, and lead accumulation), and antioxidant activities (catalase, peroxidase, superoxide dismutase) in tomato plants. Results revealed that lead contamination significantly impaired plant growth, reducing shoot and root lengths by approximately 49.78–57.62%, and negatively impacted physiological attributes and antioxidant enzyme activities. However, inoculation with PGPR strains S5 and S10 led to notable improvements in growth parameters, enhanced physiological traits, and better antioxidant activities, effectively mitigating lead stress. These findings highlights the potential of PGPR to improve tomato plant resilience to lead contamination. |
| format | Article |
| id | doaj-art-9e8cfa9890db45e5a5fbbb8340eec6c9 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-9e8cfa9890db45e5a5fbbb8340eec6c92025-01-05T12:21:31ZengNature PortfolioScientific Reports2045-23222025-01-011511910.1038/s41598-024-84648-xImpact of rhizobacterial inoculation in plant growth medium to mitigate lead stress in Tomato (Solanum lycopersicum L.)Sumreen Anjum0Mubeen Sarwar1Adnan Mukhtar2Tanveer Hussain3Mehwish Liaquat4Alia Batool5Muhammad Waqar Alam6Qurban Ali7M. Irfan Ashraf8Institutes of Botany, Faculty of Life Sciences, University of the PunjabDepartment of Horticulture, University of the PunjabDepartment of Food Science, University of AgricultureDepartment of Horticulture, PMAS Arid Agriculture University RawalpindiDepartment of Horticulture, PMAS Arid Agriculture University RawalpindiDepartment of Plant Breeding & Genetics, University of the PunjabDepartment of Plant Pathology, University of OkaraInstitutes of Botany, Faculty of Life Sciences, University of the PunjabInstitute of Horticultural Sciences, University of AgricultureAbstract This study investigates the effects of lead stress on tomato plants and explores the potential role of plant growth-promoting rhizobacteria (PGPR) to alleviate this stress. The experiment was conducted in pots, introducing varying lead levels (0, 100, 200, 300, 400, and 500 mg kg⁻¹) using lead nitrate. For rhizobacterial inoculation, pre-characterized LTPGP strains S5 Pseudomonas fluorescens A506 and S10 Pseudomonas fluorescens LMG 2189 were used. Data were recorded on growth parameters (shoot and root length, fresh and dry weight), physiological attributes (chlorophyll content, proline content, electrolyte leakage, and lead accumulation), and antioxidant activities (catalase, peroxidase, superoxide dismutase) in tomato plants. Results revealed that lead contamination significantly impaired plant growth, reducing shoot and root lengths by approximately 49.78–57.62%, and negatively impacted physiological attributes and antioxidant enzyme activities. However, inoculation with PGPR strains S5 and S10 led to notable improvements in growth parameters, enhanced physiological traits, and better antioxidant activities, effectively mitigating lead stress. These findings highlights the potential of PGPR to improve tomato plant resilience to lead contamination.https://doi.org/10.1038/s41598-024-84648-xPlant growth-promoting rhizobacteria (PGPR)Pseudomonas fluorescensGrowth parametersPhysiological attributesAntioxidant enzyme activityLead accumulation |
| spellingShingle | Sumreen Anjum Mubeen Sarwar Adnan Mukhtar Tanveer Hussain Mehwish Liaquat Alia Batool Muhammad Waqar Alam Qurban Ali M. Irfan Ashraf Impact of rhizobacterial inoculation in plant growth medium to mitigate lead stress in Tomato (Solanum lycopersicum L.) Scientific Reports Plant growth-promoting rhizobacteria (PGPR) Pseudomonas fluorescens Growth parameters Physiological attributes Antioxidant enzyme activity Lead accumulation |
| title | Impact of rhizobacterial inoculation in plant growth medium to mitigate lead stress in Tomato (Solanum lycopersicum L.) |
| title_full | Impact of rhizobacterial inoculation in plant growth medium to mitigate lead stress in Tomato (Solanum lycopersicum L.) |
| title_fullStr | Impact of rhizobacterial inoculation in plant growth medium to mitigate lead stress in Tomato (Solanum lycopersicum L.) |
| title_full_unstemmed | Impact of rhizobacterial inoculation in plant growth medium to mitigate lead stress in Tomato (Solanum lycopersicum L.) |
| title_short | Impact of rhizobacterial inoculation in plant growth medium to mitigate lead stress in Tomato (Solanum lycopersicum L.) |
| title_sort | impact of rhizobacterial inoculation in plant growth medium to mitigate lead stress in tomato solanum lycopersicum l |
| topic | Plant growth-promoting rhizobacteria (PGPR) Pseudomonas fluorescens Growth parameters Physiological attributes Antioxidant enzyme activity Lead accumulation |
| url | https://doi.org/10.1038/s41598-024-84648-x |
| work_keys_str_mv | AT sumreenanjum impactofrhizobacterialinoculationinplantgrowthmediumtomitigateleadstressintomatosolanumlycopersicuml AT mubeensarwar impactofrhizobacterialinoculationinplantgrowthmediumtomitigateleadstressintomatosolanumlycopersicuml AT adnanmukhtar impactofrhizobacterialinoculationinplantgrowthmediumtomitigateleadstressintomatosolanumlycopersicuml AT tanveerhussain impactofrhizobacterialinoculationinplantgrowthmediumtomitigateleadstressintomatosolanumlycopersicuml AT mehwishliaquat impactofrhizobacterialinoculationinplantgrowthmediumtomitigateleadstressintomatosolanumlycopersicuml AT aliabatool impactofrhizobacterialinoculationinplantgrowthmediumtomitigateleadstressintomatosolanumlycopersicuml AT muhammadwaqaralam impactofrhizobacterialinoculationinplantgrowthmediumtomitigateleadstressintomatosolanumlycopersicuml AT qurbanali impactofrhizobacterialinoculationinplantgrowthmediumtomitigateleadstressintomatosolanumlycopersicuml AT mirfanashraf impactofrhizobacterialinoculationinplantgrowthmediumtomitigateleadstressintomatosolanumlycopersicuml |