Nesfatin-1 as a crucial mediator of glucose homeostasis in the reptile, Hemidactylus flaviviridis
Abstract Nesfatin-1 is a crucial regulator of energy homeostasis in mammals and fishes, however, its metabolic role remains completely unexplored in amphibians, reptiles, and birds. Therefore, present study elucidates role of nesfatin-1 in glucose homeostasis in wall lizard wherein fasting stimulate...
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Nature Portfolio
2024-12-01
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| Online Access: | https://doi.org/10.1038/s41598-024-74371-y |
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| author | Krittika Dotania Mamta Tripathy Umesh Rai |
| author_facet | Krittika Dotania Mamta Tripathy Umesh Rai |
| author_sort | Krittika Dotania |
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| description | Abstract Nesfatin-1 is a crucial regulator of energy homeostasis in mammals and fishes, however, its metabolic role remains completely unexplored in amphibians, reptiles, and birds. Therefore, present study elucidates role of nesfatin-1 in glucose homeostasis in wall lizard wherein fasting stimulated hepatic nucb2/nesfatin-1, glycogen phosphorylase (glyp), phosphoenolpyruvate carboxykinase (pepck), and fructose 1,6-bisphosphatase (fbp), while feeding upregulated pancreatic nucb2/nesfatin-1 and insulin, suggesting towards tissue-specific dual role of nesfatin-1 in glucoregulation. The glycogenolytic/gluconeogenic role of nesfatin-1 was further confirmed by an increase in media glucose levels along with heightened hepatic pepck and fbp expression and concomitant decline in liver glycogen content in nesfatin-1-treated liver of wall lizard. Moreover, treatment with nesfatin-1 stimulated insulin expression in pancreas while insulin downregulated pancreatic nucb2/nesfatin-1. Further, prolonged fasting induced elevated nucb2/nesfatin-1, and lipolytic markers, adipose triglyceride lipase (atgl) and monoglyceride lipase (mgl) in adipose tissue implicate nesfatin-1 in lipolysis which is substantiated by nesfatin-1-mediated direct upregulation of atgl and mgl. Our study provides the first comprehensive overview of tissue-dependent role of nesfatin-1 in regulating energy homeostasis in a reptile. |
| format | Article |
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| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-9e6266a98289455c932f811201c50e5f2025-01-05T12:28:03ZengNature PortfolioScientific Reports2045-23222024-12-0114111310.1038/s41598-024-74371-yNesfatin-1 as a crucial mediator of glucose homeostasis in the reptile, Hemidactylus flaviviridisKrittika Dotania0Mamta Tripathy1Umesh Rai2Department of Zoology, University of DelhiDepartment of Zoology, University of DelhiUniversity of JammuAbstract Nesfatin-1 is a crucial regulator of energy homeostasis in mammals and fishes, however, its metabolic role remains completely unexplored in amphibians, reptiles, and birds. Therefore, present study elucidates role of nesfatin-1 in glucose homeostasis in wall lizard wherein fasting stimulated hepatic nucb2/nesfatin-1, glycogen phosphorylase (glyp), phosphoenolpyruvate carboxykinase (pepck), and fructose 1,6-bisphosphatase (fbp), while feeding upregulated pancreatic nucb2/nesfatin-1 and insulin, suggesting towards tissue-specific dual role of nesfatin-1 in glucoregulation. The glycogenolytic/gluconeogenic role of nesfatin-1 was further confirmed by an increase in media glucose levels along with heightened hepatic pepck and fbp expression and concomitant decline in liver glycogen content in nesfatin-1-treated liver of wall lizard. Moreover, treatment with nesfatin-1 stimulated insulin expression in pancreas while insulin downregulated pancreatic nucb2/nesfatin-1. Further, prolonged fasting induced elevated nucb2/nesfatin-1, and lipolytic markers, adipose triglyceride lipase (atgl) and monoglyceride lipase (mgl) in adipose tissue implicate nesfatin-1 in lipolysis which is substantiated by nesfatin-1-mediated direct upregulation of atgl and mgl. Our study provides the first comprehensive overview of tissue-dependent role of nesfatin-1 in regulating energy homeostasis in a reptile.https://doi.org/10.1038/s41598-024-74371-yNesfatin-1Wall lizardGlucoregulationCarbohydrate metabolismLipolysis |
| spellingShingle | Krittika Dotania Mamta Tripathy Umesh Rai Nesfatin-1 as a crucial mediator of glucose homeostasis in the reptile, Hemidactylus flaviviridis Scientific Reports Nesfatin-1 Wall lizard Glucoregulation Carbohydrate metabolism Lipolysis |
| title | Nesfatin-1 as a crucial mediator of glucose homeostasis in the reptile, Hemidactylus flaviviridis |
| title_full | Nesfatin-1 as a crucial mediator of glucose homeostasis in the reptile, Hemidactylus flaviviridis |
| title_fullStr | Nesfatin-1 as a crucial mediator of glucose homeostasis in the reptile, Hemidactylus flaviviridis |
| title_full_unstemmed | Nesfatin-1 as a crucial mediator of glucose homeostasis in the reptile, Hemidactylus flaviviridis |
| title_short | Nesfatin-1 as a crucial mediator of glucose homeostasis in the reptile, Hemidactylus flaviviridis |
| title_sort | nesfatin 1 as a crucial mediator of glucose homeostasis in the reptile hemidactylus flaviviridis |
| topic | Nesfatin-1 Wall lizard Glucoregulation Carbohydrate metabolism Lipolysis |
| url | https://doi.org/10.1038/s41598-024-74371-y |
| work_keys_str_mv | AT krittikadotania nesfatin1asacrucialmediatorofglucosehomeostasisinthereptilehemidactylusflaviviridis AT mamtatripathy nesfatin1asacrucialmediatorofglucosehomeostasisinthereptilehemidactylusflaviviridis AT umeshrai nesfatin1asacrucialmediatorofglucosehomeostasisinthereptilehemidactylusflaviviridis |