Preclinical evaluation of DC-CIK cells as potentially effective immunotherapy model for the treatment of glioblastoma
Abstract Despite the favorable effects of immunotherapies in multiple types of cancers, its complete success in CNS malignancies remains challenging. Recently, a successful clinical trial of cytokine-induced killer (CIK) cell immunotherapy in patients with glioblastoma (GBM) has opened a new avenue...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-84284-5 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559611512979456 |
|---|---|
| author | Annika Simone Lück Jingjing Pu Ahmad Melhem Matthias Schneider Amit Sharma Ingo G. H. Schmidt-Wolf Jarek Maciaczyk |
| author_facet | Annika Simone Lück Jingjing Pu Ahmad Melhem Matthias Schneider Amit Sharma Ingo G. H. Schmidt-Wolf Jarek Maciaczyk |
| author_sort | Annika Simone Lück |
| collection | DOAJ |
| description | Abstract Despite the favorable effects of immunotherapies in multiple types of cancers, its complete success in CNS malignancies remains challenging. Recently, a successful clinical trial of cytokine-induced killer (CIK) cell immunotherapy in patients with glioblastoma (GBM) has opened a new avenue for adoptive cellular immunotherapies in CNS malignancies. Prompt from these findings, herein, we investigated whether dendritic cells (DC) in combination with cytokine-induced killer cells (DC-CIK) could also provide an alternative and more effective way to improve the efficacy of GBM treatment. The analysis showed that DC-CIK cells exerted a significant cytotoxic effect on the glioblastoma cell lines, especially with the phenotype of stem-like cells (GSCs). In addition, the increased specific lysis of these cells subsequent to DC-CIK co-culture was confirmed with confocal fluorescence microscope. The direct interactions between tumor and effector cells were found to be highly effective in GBM organoids (GBOs). Moreover, a significant increase in apoptosis and elevated levels of IFN-γ (and not TNF-α) secretion were observed as a targeting mechanism of DC-CIK cells against GBM cell models. Overall, we provide important preliminary evidence that DC-CIK cells may have potential in the treatment of CNS malignancies, particularly glioblastoma. |
| format | Article |
| id | doaj-art-9e30bfef41484364aa045e52951dcfbe |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-9e30bfef41484364aa045e52951dcfbe2025-01-05T12:21:11ZengNature PortfolioScientific Reports2045-23222025-01-0115111010.1038/s41598-024-84284-5Preclinical evaluation of DC-CIK cells as potentially effective immunotherapy model for the treatment of glioblastomaAnnika Simone Lück0Jingjing Pu1Ahmad Melhem2Matthias Schneider3Amit Sharma4Ingo G. H. Schmidt-Wolf5Jarek Maciaczyk6Department of Stereotactic and Functional Neurosurgery, University Hospital of BonnDepartment of Integrated Oncology, Center for Integrated Oncology (CIO), University Hospital of BonnDepartment of Neurosurgery, University Hospital BonnDepartment of Neurosurgery, University Hospital BonnDepartment of Stereotactic and Functional Neurosurgery, University Hospital of BonnDepartment of Integrated Oncology, Center for Integrated Oncology (CIO), University Hospital of BonnDepartment of Stereotactic and Functional Neurosurgery, University Hospital of BonnAbstract Despite the favorable effects of immunotherapies in multiple types of cancers, its complete success in CNS malignancies remains challenging. Recently, a successful clinical trial of cytokine-induced killer (CIK) cell immunotherapy in patients with glioblastoma (GBM) has opened a new avenue for adoptive cellular immunotherapies in CNS malignancies. Prompt from these findings, herein, we investigated whether dendritic cells (DC) in combination with cytokine-induced killer cells (DC-CIK) could also provide an alternative and more effective way to improve the efficacy of GBM treatment. The analysis showed that DC-CIK cells exerted a significant cytotoxic effect on the glioblastoma cell lines, especially with the phenotype of stem-like cells (GSCs). In addition, the increased specific lysis of these cells subsequent to DC-CIK co-culture was confirmed with confocal fluorescence microscope. The direct interactions between tumor and effector cells were found to be highly effective in GBM organoids (GBOs). Moreover, a significant increase in apoptosis and elevated levels of IFN-γ (and not TNF-α) secretion were observed as a targeting mechanism of DC-CIK cells against GBM cell models. Overall, we provide important preliminary evidence that DC-CIK cells may have potential in the treatment of CNS malignancies, particularly glioblastoma.https://doi.org/10.1038/s41598-024-84284-5DC-CIK cellsCytokine-induced killer cellsImmunotherapyGlioblastoma |
| spellingShingle | Annika Simone Lück Jingjing Pu Ahmad Melhem Matthias Schneider Amit Sharma Ingo G. H. Schmidt-Wolf Jarek Maciaczyk Preclinical evaluation of DC-CIK cells as potentially effective immunotherapy model for the treatment of glioblastoma Scientific Reports DC-CIK cells Cytokine-induced killer cells Immunotherapy Glioblastoma |
| title | Preclinical evaluation of DC-CIK cells as potentially effective immunotherapy model for the treatment of glioblastoma |
| title_full | Preclinical evaluation of DC-CIK cells as potentially effective immunotherapy model for the treatment of glioblastoma |
| title_fullStr | Preclinical evaluation of DC-CIK cells as potentially effective immunotherapy model for the treatment of glioblastoma |
| title_full_unstemmed | Preclinical evaluation of DC-CIK cells as potentially effective immunotherapy model for the treatment of glioblastoma |
| title_short | Preclinical evaluation of DC-CIK cells as potentially effective immunotherapy model for the treatment of glioblastoma |
| title_sort | preclinical evaluation of dc cik cells as potentially effective immunotherapy model for the treatment of glioblastoma |
| topic | DC-CIK cells Cytokine-induced killer cells Immunotherapy Glioblastoma |
| url | https://doi.org/10.1038/s41598-024-84284-5 |
| work_keys_str_mv | AT annikasimoneluck preclinicalevaluationofdccikcellsaspotentiallyeffectiveimmunotherapymodelforthetreatmentofglioblastoma AT jingjingpu preclinicalevaluationofdccikcellsaspotentiallyeffectiveimmunotherapymodelforthetreatmentofglioblastoma AT ahmadmelhem preclinicalevaluationofdccikcellsaspotentiallyeffectiveimmunotherapymodelforthetreatmentofglioblastoma AT matthiasschneider preclinicalevaluationofdccikcellsaspotentiallyeffectiveimmunotherapymodelforthetreatmentofglioblastoma AT amitsharma preclinicalevaluationofdccikcellsaspotentiallyeffectiveimmunotherapymodelforthetreatmentofglioblastoma AT ingoghschmidtwolf preclinicalevaluationofdccikcellsaspotentiallyeffectiveimmunotherapymodelforthetreatmentofglioblastoma AT jarekmaciaczyk preclinicalevaluationofdccikcellsaspotentiallyeffectiveimmunotherapymodelforthetreatmentofglioblastoma |