Enantioseparation of Secnidazole by High-Performance Liquid Chromatography Using Amylose-Based Stationary Phase
The secnidazole, a drug used in hepatic amebiasis, giardiasis and bacterial vaginosis, was enantioseparated on a semi-preparative scale by high performance liquid chromatography (HPLC), using chiral stationary phase (CSP) containing amylose tris (3, 5-dimethylphenyl) carbamate on silica gel (Chiralp...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2012-09-01
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| Series: | Adsorption Science & Technology |
| Online Access: | https://doi.org/10.1260/0263-6174.30.8-9.739 |
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| author | Ana C. Nascimento Rafael F. Perna Marco A. Cremasco Cesar C. Santana |
| author_facet | Ana C. Nascimento Rafael F. Perna Marco A. Cremasco Cesar C. Santana |
| author_sort | Ana C. Nascimento |
| collection | DOAJ |
| description | The secnidazole, a drug used in hepatic amebiasis, giardiasis and bacterial vaginosis, was enantioseparated on a semi-preparative scale by high performance liquid chromatography (HPLC), using chiral stationary phase (CSP) containing amylose tris (3, 5-dimethylphenyl) carbamate on silica gel (Chiralpak® AD). The experiments were carried out under normal phase conditions and the separation method exhibited very high selectivity and resolutions. The chromatographic parameters and the effects of column temperature on the retention time and selectivity were evaluated. Thermodynamic parameters were obtained from van't Hoff plots, and the elution order of the enantiomers was determined by circular dichorism. The enantiomers of secnidazole were separated under overloaded conditions with a good performance. |
| format | Article |
| id | doaj-art-9e0c749835254dab964af7ac190cff78 |
| institution | Kabale University |
| issn | 0263-6174 2048-4038 |
| language | English |
| publishDate | 2012-09-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Adsorption Science & Technology |
| spelling | doaj-art-9e0c749835254dab964af7ac190cff782025-01-03T00:10:27ZengSAGE PublishingAdsorption Science & Technology0263-61742048-40382012-09-013010.1260/0263-6174.30.8-9.739Enantioseparation of Secnidazole by High-Performance Liquid Chromatography Using Amylose-Based Stationary PhaseAna C. NascimentoRafael F. PernaMarco A. CremascoCesar C. SantanaThe secnidazole, a drug used in hepatic amebiasis, giardiasis and bacterial vaginosis, was enantioseparated on a semi-preparative scale by high performance liquid chromatography (HPLC), using chiral stationary phase (CSP) containing amylose tris (3, 5-dimethylphenyl) carbamate on silica gel (Chiralpak® AD). The experiments were carried out under normal phase conditions and the separation method exhibited very high selectivity and resolutions. The chromatographic parameters and the effects of column temperature on the retention time and selectivity were evaluated. Thermodynamic parameters were obtained from van't Hoff plots, and the elution order of the enantiomers was determined by circular dichorism. The enantiomers of secnidazole were separated under overloaded conditions with a good performance.https://doi.org/10.1260/0263-6174.30.8-9.739 |
| spellingShingle | Ana C. Nascimento Rafael F. Perna Marco A. Cremasco Cesar C. Santana Enantioseparation of Secnidazole by High-Performance Liquid Chromatography Using Amylose-Based Stationary Phase Adsorption Science & Technology |
| title | Enantioseparation of Secnidazole by High-Performance Liquid Chromatography Using Amylose-Based Stationary Phase |
| title_full | Enantioseparation of Secnidazole by High-Performance Liquid Chromatography Using Amylose-Based Stationary Phase |
| title_fullStr | Enantioseparation of Secnidazole by High-Performance Liquid Chromatography Using Amylose-Based Stationary Phase |
| title_full_unstemmed | Enantioseparation of Secnidazole by High-Performance Liquid Chromatography Using Amylose-Based Stationary Phase |
| title_short | Enantioseparation of Secnidazole by High-Performance Liquid Chromatography Using Amylose-Based Stationary Phase |
| title_sort | enantioseparation of secnidazole by high performance liquid chromatography using amylose based stationary phase |
| url | https://doi.org/10.1260/0263-6174.30.8-9.739 |
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