Urea supplementation improves mRNA in vitro transcription by decreasing both shorter and longer RNA byproducts

Urea supplementation improves mRNA in vitro transcription by decreasing both shorter and longer RNA byproducts

The current letter to the editor describes the presence of RNA byproducts in small-scale in vitro transcription (IVT) reactions as evaluated by capillary gel electrophoresis, asymmetric flow field flow fractionation, immunoblotting, cell-free translation assays, and in IFN reporter cells. We compare...

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Bibliographic Details
Main Authors: Combes Francis, Pettersson Frida J, Bui Thanh-Huong, Molska Alicja, Komissarov Artem, Parot Jérémie, Borgos Sven Even
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:RNA Biology
Subjects:
In vitro transcription
urea
VSW3
RNA polymerase
transcript impurities
mRNA byproducts
Online Access:https://www.tandfonline.com/doi/10.1080/15476286.2024.2321764
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